Colonic chyme of piglet
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1110031 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Dietary berberine alleviates enterotoxigenic Escherichia coli (ETEC) infection induced intestinal mucosal barrier injury by optimizes the intestinal microbial community of weaned Piglets
| PRJNA1110050 | ENA
Project description:Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in children in resource-limited countries and of travellers diarrhoea. The ileal proteomics change after ETEC challenge is less characterised. Here in this study changes of ileal proteins post ETEC challenge in weaned pigs are studied. In total, 5151 ileal proteins were successfully annotated and 9 proteins had significantly different abundance between the ETEC and CON pigs.
2022-04-01 | PXD028066 | Pride
Project description:The aim of this study was to characterize the metabolomic and proteomic changes in the intestinal tissue of weaned piglets fed with diets containing Spirulina as an ingredient (10% incorporation in the diet), combining such information with histomorphology data.
2022-09-20 | PXD030446 | Pride
Project description:Enterotoxigenic Escherichia coli (ETEC) strains that produce both heat-stable (ST) and heat-labile (LT) enterotoxins cause severe post-weaning diarrhea in piglets. However, the relative importance of the individual enterotoxins to the pathogenesis of ETEC infection is poorly understood. In this study, we investigated the effect on virulence of an F4+ ETEC strain when removing some or all of its enterotoxins. Several isogenic mutant strains were constructed that lack the expression of LT in combination with one or both types of ST enterotoxins (STa and/or STb). Host early immune responses induced by these mutant strains 4h after infection were compared to the wild type strain GIS26 Â (O149:F4ac+, Â LT+ Â STa+ Â STb+). At the same time, the immune response of this wild type ETEC strain was compared to the mock-infected control, demonstrating the expression of porcine inflammatory response genes. For these purposes, the small intestinal segment perfusion (SISP) technique and microarray analysis were used and results were validated by qRT-PCR. We also measured net fluid absorption of pig small intestinal mucosa 4h after infection with wild type ETEC, the mutant strains and PBS (mock-infected). These data indicate an important role for STb in inducing small intestinal secretion early after infection. The microarray analysis of the different mutant strains also revealed an important role for STb in ETEC-induced immune response by the significant differential regulation of immune mediators like matrix metalloproteinase 3, interleukin 1 and interleukin 17. We conclude that STb can play a prominent role in ETEC-induced secretion and early immune response. In three pigs, 6 different treatments were performed. These treatments consisted of 4 mutant enterotoxigenic Escherichia coli GIS26 strains, GIS26 wild type strain, or PBS control. Per pig, the small intestine was divided into 6 loops with an interloop in between to avoid cross-contamination. In conclusion, every pig received each of the 6 treatments ad random.
2013-01-02 | E-GEOD-28003 | biostudies-arrayexpress
Project description:To better understand the molecular effects of Enterotoxigenic Escherichia coli (ETEC) F4ab/ac infection, we performed a genome-wide comparison of the changes in DNA methylation in ETEC F4ab/ac infected porcine intestinal epithelial cells. Our data provides further insight into the epigenetic alterations of ETEC F4ab/ac infected porcine intestinal epithelial cells and may advance the identification of biomarkers and drug targets for predicting susceptibility to and controlling ETEC F4ab/ac induced diarrhea.
2020-04-06 | GSE143661 | GEO
Project description:<p>Abstract:</p><p>Background: Quercetin is a flavonoid with various probiotic properties, demonstrating anti-inflammatory, anti-oxidative, anti-cancer, and anti-obesity effects in humans and animals. However, quercetin’s effect on enterotoxigenic Escherichia coli (ETEC) K88-induced host body injury mediated by bacterial quorum sensing remains insufficiently explored.</p><p>Results: Quercetin supplementation alleviated ETEC K88-induced body damage in weaned piglets, changed the microbial composition of the host intestinal tract, enriched the main probiotic Lactobacillus amylovorus, and inhibited microbial functions, such as virulence factor secretion. In addition, it influenced changes in AHL-type quorum-sensing signaling molecules in the host circulation. The signaling pathway alleviated ETEC K88-induced host intestinal barrier and inflammatory damage.</p><p>Conclusion: This study provides a theoretical framework for further understanding and exploring the use of quorum sensing as a medium to alleviate human and animal diseases caused by pathogenic bacteria and other external adverse factors.</p>
2026-01-14 | MTBLS13677 | MetaboLights
Project description:Enterotoxigenic Escherichia coli (ETEC) strains that produce both heat-stable (ST) and heat-labile (LT) enterotoxins cause severe post-weaning diarrhea in piglets. However, the relative importance of the individual enterotoxins to the pathogenesis of ETEC infection is poorly understood. In this study, we investigated the effect on virulence of an F4+ ETEC strain when removing some or all of its enterotoxins. Several isogenic mutant strains were constructed that lack the expression of LT in combination with one or both types of ST enterotoxins (STa and/or STb). Host early immune responses induced by these mutant strains 4h after infection were compared to the wild type strain GIS26 (O149:F4ac+, LT+ STa+ STb+). At the same time, the immune response of this wild type ETEC strain was compared to the mock-infected control, demonstrating the expression of porcine inflammatory response genes. For these purposes, the small intestinal segment perfusion (SISP) technique and microarray analysis were used and results were validated by qRT-PCR. We also measured net fluid absorption of pig small intestinal mucosa 4h after infection with wild type ETEC, the mutant strains and PBS (mock-infected). These data indicate an important role for STb in inducing small intestinal secretion early after infection. The microarray analysis of the different mutant strains also revealed an important role for STb in ETEC-induced immune response by the significant differential regulation of immune mediators like matrix metalloproteinase 3, interleukin 1 and interleukin 17. We conclude that STb can play a prominent role in ETEC-induced secretion and early immune response.
2013-01-02 | GSE28003 | GEO
Project description:engineered bacillus subtilis protect the weaned piglets from ETEC K88 infection through improving serum metabolites, intestinal imcrobiota, antioxidant and immune activity.
2026-02-23 | MTBLS7587 | MetaboLights
Project description:Colonic chyme of piglets Metagenome
| PRJNA1110045 | ENA
Project description:This SuperSeries is composed of the following subset Series: GSE31804: Expression data of enterotoxigenic (ETEC) isolate E24377A GSE31805: Global transcriptomics of enterotoxigenic E. coli strain E24377A Refer to individual Series
2012-03-01 | E-GEOD-31806 | biostudies-arrayexpress