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Direct RNA sequencing experiment of major tissues from wild-type mice under fasting and fed treatments

ABSTRACT: Direct RNA sequencing experiment of major tissues from wild-type mice under fasting and fed treatments

PROVIDER: PRJNA1110982 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29006031_1.fastq.gz	Fastqsanger.gz
	SRR29006032_1.fastq.gz	Fastqsanger.gz
	SRR29006033_1.fastq.gz	Fastqsanger.gz
	SRR29006034_1.fastq.gz	Fastqsanger.gz
	SRR29006035_1.fastq.gz	Fastqsanger.gz

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Direct RNA sequencing experiment of major tissues from wild-type mice under fasting and fed treatments

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ATAC-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

Project description:Male C57BL/6 mice, sourced from the Jackson Laboratory, were acquired at 12 weeks of age. The mice were housed in groups of three per cage, with unrestricted access to water and a standard chow diet. For the experimental treatments involving fasting and fed states (n = 3 per group), the mice were either provided continuous access to food or subjected to a 24-hour fasting period before euthanasia at 10 am for tissue collection. The tissues harvested included muscle, liver, heart, lungs, both white and brown adipose tissues, spleen, and kidney. These tissues were immediately preserved in liquid nitrogen post-sacrifice to maintain their integrity for subsequent analyses.

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Short-read RNA-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

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ATAC-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

Project description:ATAC-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

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Short-read RNA-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

Project description:Short-read RNA-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

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Fasting Fed RNA sequencing experiment of mice with humanized livers

Project description:We report the RNA sequencing results of four fasting mice with humanized livers and four fed mice with humanized livers.

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Direct RNA sequencing experiment of humanized liver mice with metabolic treatments

Project description:To make the human liver accessible to metabolic treatments, we employed a liver-specific humanized mouse model in which approximately 50% of the mouse hepatocytes were replaced by human ones. To capture transcriptomes reflecting pathophysiology and therapeutic development of metabolic diseases, we subjected the humanized mice to dietary intervention and the key metabolic transcriptional factor agonist treatments. We then performed rna exoression profiling analysis using data obtained from nanopore direct RNA sequencing of these humanized mice.

2024-01-23 | GSE224278 | GEO

Direct RNA sequencing experiment of humanized liver mice with metabolic treatments

Project description:Direct RNA sequencing experiment of humanized liver mice with metabolic treatments

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Fasting/fed/refeeding RNAseq experiment conducted on mice with xenografted human livers.

Project description:Human hepatic gene regulations by fasting and refeeding in a mouse model with humanized liver generated from a single donor were reported.

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ATAC-Seq experiment of humanized liver mice with metabolic treatments

Project description:To make the human liver accessible to metabolic treatments, we employed a liver-specific humanized mouse model in which approximately 50% of the mouse hepatocytes were replaced by human ones. For the dietary treatment, the humanized mice were allowed free access to food (AL, n=4 for donor1, n=3 for donor2) or subjected to a twenty-four hours food withdrawal (Fast, n=4 for donor1, n=3 for donor2). For the transcription factor agonist treatments, the humanized mice were injected with DMSO (n=4), fenofibrate (n=4, 50mg/kg, Sigma-Aldrich, Cat. F6020), rosiglitazone (n=4,10mg/kg, Sigma-Aldrich, Cat. R2408) and GW4064 (n=4, 30mg/kg, Sigma-Aldrich, Cat. G5172) by i.p. injection. The livers were collected after 6 hours fasting and stored in liquid nitrogen immediately after mice sacrificed.

2024-01-23 | GSE224277 | GEO

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