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Effect of B cell receptor class-switching on WSU-FSCCL lymphoma cells

ABSTRACT: Effect of B cell receptor class-switching on WSU-FSCCL lymphoma cells

PROVIDER: PRJNA1111512 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29022800_1.fastq.gz	Fastqsanger.gz
	SRR29022800_2.fastq.gz	Fastqsanger.gz
	SRR29022801_1.fastq.gz	Fastqsanger.gz
	SRR29022801_2.fastq.gz	Fastqsanger.gz
	SRR29022802_1.fastq.gz	Fastqsanger.gz

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Effect of B cell receptor class-switching on WSU-FSCCL lymphoma cells

Project description:Although immunoglobulin class-switching from IgM to IgG enhances B cell receptor (BCR) signalling and antibody responses, certain subsets of B cell lymphomas retain unswitched IgM BCRs despite signs of class-switching in the immunoglobulin heavy chain loci. This suggests that expression of the IgG BCR suppresses progression of specific lymphoma subsets. We found that inducing BCR class switching from IgM to IgG1 or IgG4 inhibits proliferation of B EZB DLBCL lymphoma cell lines. To understand changes associated with class-switching we compared transcritional profiles of isogenic WSU-FSCCL cell lines artificially class-switched to express IgM and IgG BCRs.

2024-05-15 | GSE267440 | GEO

Chromatin remodeling in HIF-1a deficient B cell during IgA class switching [ChIP-seq]

Project description:The interconnection between metabolic programming and chromatin remodeling during IgA class switching suggest that HIF-1a-dependent glycolytic flux may control the epigenetic modification at α class switching region (Sα) in the Igh locus. To test our hypothesis, histone acetylation was analyzed by chromatin immunoprecipitation sequencing (ChIP-seq) (H3K9ac and H3K27ac) in Hif-1a deficient and control B cell during IgA class switching. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27ac and H3K9ac in B cell from WT and HIF-1a deficient mice.

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Single-cell RNA and BCR sequencing of naive B cells cultured in IgG class-switching polarising conditions

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Intrinsic transcriptional heterogeneity in B cells controls early class switching to IgE

Project description:'Non-coding transcripts originating upstream of the immunoglobulin constant region (I-transcripts) are required to direct activation-induced deaminase to initiate class switching in B cells. Differential regulation of I and I1 transcription in response to interleukin-4 (IL-4), hence class switching to IgE and IgG1, is not fully understood. Here we combine novel mouse reporters and single-cell RNA-seq to reveal the heterogeneity in IL-4 induced I-transcription. Transgenic mice were generated with one allele labelled with an eGFP fluorescent reporter gene in the first exon of the gamma-1 non-coding RNA and one allele with a tdTom fluorescent reporter gene in the first exon of the epsilon non-coding RNA. Ex vivo splenic B cells from these mice were stimulated with IL-4, lipopolysaccharide and B cell activating factor. After 24 hours culture, cell were sorted according to their fluorescence and used for single-cell mRNA-sequencing. Two independent experiments were performed (labelled as ''batch 1'' and ''batch 2'').'

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Project description:We performed CSR-HTGTS-Seq, 3C-HTGTS, GRO-Seq and ChIP-Seq in mature splenic B cells with different stimulation study physiological roles of 3'CBEs in antibody class switching

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Project description:We performed CSR-HTGTS-Seq, 3C-HTGTS, GRO-Seq and ChIP-Seq in mature splenic B cells with different stimulation study physiological roles of 3'CBEs in antibody class switching

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Effect of FDX1 overexpression on gene expression in WSU-DLCL2 cells upon elesclomol stimulation

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The physiological role of 3’CBEs in antibody class switching

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