Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.
Project description:We report the whole genome sequence of Mycolicibacterium parafortuitum strain Panama NTM1, isolated from cattle feces at a dairy farm in Panama (8°08'18.1″N and 80°54'00.1″W). DNA was extracted from a pure culture of this isolate and whole-genome sequencing was performed using the Illumina MiSeqⓇ platform. After de novo assembly, the genome has a total size of 5.92 Mbp, a GC content of 68.4 %, and 5545 annotated genes. The raw read files and genome have been deposited in the NCBI database under BioProject number PRJNA1113557.
Project description:We identified a novel transcription factor, MsoA, in response to oxidative stress in Mycolicibacterium smegmatis. We hope to discover the regulatory network and target genes of transcription factor msoA by transcriptome sequencing of wild-type and msoA mutants, so as to help us further elucidate the molecular mechanism of msoA involved in regulation of Mycolicibacterium oxidation.