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Differential gene expression between gastric cancer cells treated with recombinant human growth-arrest specific 6 (GAS6) protein or cultured with Cancer-associated fibroblasts (CAFs).

ABSTRACT: Differential gene expression between gastric cancer cells treated with recombinant human growth-arrest specific 6 (GAS6) protein or cultured with Cancer-associated fibroblasts (CAFs).

PROVIDER: PRJNA1114636 | ENA |

REPOSITORIES: ENA

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Differential gene expression between gastric cancer cells treated with recombinant human growth-arrest specific 6 (GAS6) protein or cultured with Cancer-associated fibroblasts (CAFs).

Project description:We aimed to determine the differences in gene expression levels between SNU668 cells and SNU668 cells co-cultured with CAFs or treated with recombinant human GAS6 protein using microarray analysis. We selected genes that showed a fold change increase of more than 2-fold compared to WT in SNU668 cells. Then, we identified genes involved in chemoresistance.

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Differential Gene Expression between YTN16 Gas6 Cells and YTN16 Cells

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Gene expression in wild type and Gas6-/- endothelial cells co-cultured with cancer cells

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Gas6/Axl signaling activates Akt in liver cancer cells

Project description:We investigated which signaling pathways downstream of Gas6/Axl promote the invasive phenotype of liver cancer cells. Thus, we performed phospho-proteomic analysis of MR hepatocytes and those expressing Axl (MR-Axl) in the presence and absence of Gas6. Protein-clustering based on the pattern of phosphorylation across the conditions and samples revealed four clusters: phospho-sites that are upregulated dependent on Axl expression (cluster 1) or Gas6 expression (cluster 2) and phospho-sites that are downregulated dependent on Axl expression (cluster 3) or Gas6 expression (cluster 4). Functional enrichment analysis showed enrichment of protein sets associated with cellular polarity and motility depending on changes in Axl expression and Gas6 activation. Furthermore, we analyzed phospho-proteomic data to estimate the activity of kinases based on kinase-substrate interactions using PhosR. Kinase perturbation analysis indicated mammalian target of rapamycin (mTOR) as the one with the highest upregulated activity in Gas6-stimulated MR-Axl cells compared to Gas6-stimulated MR cells. Accordingly, we analyzed substrates of mTOR and found that phosphorylation of Akt1 (Ser473) was upregulated in cluster 1 and highly increased in Gas6-stimulated MR-Axl, suggesting that Akt is regulated by Axl.

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Gas6

Project description:Rattus norvegicus Gas6, growth arrest specific 6 [Source:RGD Symbol;Acc:61913], is differentially expressed in 25 experiment(s);

| ENSRNOG00000018233 | ExpressionAtlas

GAS6

Project description:Monodelphis domestica GAS6, growth arrest specific 6 [Source:NCBI gene;Acc:100021343], is expressed in 2 baseline experiment(s);

| ENSMODG00000003633 | ExpressionAtlas

Gas6

Project description:Rattus norvegicus Gas6, growth arrest specific 6 [Source:RGD Symbol;Acc:61913], is expressed in 6 baseline experiment(s);

| ENSRNOG00000018233 | ExpressionAtlas

A cancer associated fibroblasts (CAFs) specific gene signature in high grade serous ovarian cancer

Project description:Ovarian cancer is the most lethal malignancy in the United States. In the year 2012, there will be an estimated 22,280 new cases and 15,500 deaths from ovarian cancer in the country (Siegel et al., 2012). While studies on ovarian cancer pathogenesis were mainly focused on the epithelial component of the tumor, understanding in the role of cancer associated fibroblasts (CAFs) in ovarian cancer progression is limited. We hypothesized that comparing the gene expression profiles of different components from laser capture microdissected ovarian tissue will allow us to identify an ovarian CAFs specific gene signature which accounts for the supportive role of CAFs in ovarian cancer progression. In this study, gene expression profiling was completed for 31 cancer stroma samples and 32 samples of epithelial component from high grade serous ovarian cancer patients. 8 microdissected normal ovarian stroma and 6 normal human ovarian surface epithelium (HOSE) samples were also included in the study. By comparing the expression data from cancer stroma against that from normal stroma, cancer cells and HOSE, we identified a set of differential expressed genes in ovarian CAFs which showed correlation with cancer patient survival. Further study on these genes can reveal their roles in ovarian cancer progression and pathogenesis. Ultimately, ovarian CAFs specified genes identified in this study may aid in the classification and enhancement of patient outcome. Transcriptome profiling analyses were performed on 31 laser microdissected cancer associated stroma samples, 32 epithelial tumor samples from high grade serous ovarian cancer patients, 8 microdissected normal ovarian stroma samples and 6 ovarian surface epthelium (HOSE) samples using the Affymetrix human genome U133 Plus 2.0 microarray.

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Project description:Sus scrofa GAS6, growth arrest specific 6 [Source:VGNC Symbol;Acc:VGNC:88358], is differentially expressed in 4 experiment(s);

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Project description:Sus scrofa GAS6, growth arrest specific 6 [Source:VGNC Symbol;Acc:VGNC:88358], is expressed in 2 baseline experiment(s);

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