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ABSTRACT: ITS RNA-Seq of rhizosphere soil microbiome of Panax notoginseng

PROVIDER: PRJNA1114936 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29183700_1.fastq.gz	Fastqsanger.gz
	SRR29183700_2.fastq.gz	Fastqsanger.gz
	SRR29183701_1.fastq.gz	Fastqsanger.gz
	SRR29183701_2.fastq.gz	Fastqsanger.gz
	SRR29183702_1.fastq.gz	Fastqsanger.gz

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Effect of Panax Notoginseng on gene expression in rats with cerebral ischemia-reperfusion injury

Project description:Methods: Panax notoginseng was used to treat MCAO model rats, and the differentially expressed genes between Panax notoginseng group and model group were identified by RNA SEQ, and the possible mechanism of Panax notoginseng in regulating ischemic stroke was analyzed

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Nitrate fertilizer alleviates ammonium toxicity by enhancing ACLA-3 in Panax notoginseng roots

Project description:Nitrogen is one of the essential elements for plant growth. NH4+ and NO3- are two major forms of absorbing element N for higher plants. In this study we found that the growth of Panax notoginseng is inhibited when only adding ammonium nitrogen fertilizer, and adding nitrate fertilizer can alleviate the toxicity caused by ammonium. We use RNA-seq to identify genes that are related to the alleviated phenotypes after introducing NO3- to Panax notoginseng roots under NH4+ stresses. Twelve RNA-seq profiles in four sample groups, i.e., control, samples treated with NH4+, samples treated with NO3- only, and treated with both NH4+ and NO3- were obtained and analyzed to identify deregulated genes in samples with different treatments. ACLA-3 gene is downregulated in NH4+ treated samples, but is upregulated in samples treated with NO3- and with both NH4+ and NO3-, which is further validated in another set of samples using qRT-PCR. Our results suggest that unbalanced metabolism of nitrogen and nitrogen is the main cause of ammonium poisoning in roots of Panax notoginseng, and NO3- may significantly upregulate the activity of ACLA-3 which subsequently enhances the citrate cycle and many other metabolic pathways in Panax notoginseng root. These potentially increase the integrity of the Panax notoginseng roots. Our results suggest that introducing NO3- fertilizer is an effective means to prevent the occurrence of toxic ammonium in Panax notoginseng root.

2019-12-31 | GSE112437 | GEO

Project description:16s RNA-Seq of rhizosphere soil microbiome of Panax notoginseng

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Project description:18S RNA-Seq of rhizosphere soil microbiome of Panax notoginseng

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RNA illumina sequencing of Panax notoginseng under CK and Cd stress treatment

Project description:The total RNA were extracted from tissues of roots from several plants of Panax notoginseng under CK and Cd stress treatment by using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The purified PCR product was sequenced using Illumina Genome Analyzer II. The qualified reads were used to study of Panax notoginseng transcriptome under CK and Cd stress treatment.

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Project description:Panax notoginseng rhizosphere microbiome sequencing

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Transcriptomics provides novel insights into the regulatory mechanism of Panax notoginseng exosome-like nanoparticles in HSF

Project description:In current study, we observed that Panax notoginseng exosome-like nanoparticles successfully inhibited apoptosis and promoted proliferation in HSF. Moreover, transcriptomics was implemented to uncover its underlying mechanism. A total of 7924 differentially expressed genes (DEGs) were identified, with some of them enriched in apoptosis, proliferation related pathways. Intriguingly, some DEGs were annotated in skin aging development or coagulation function pathways. In view of this, we suggested Panax notoginseng exosomes may delay skin aging. Lastly, RT-qPCR confirmed the veracity and reliability of RNA-sequencing results.

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Project description:Rhizosphere microbiome of Panax notoginseng (HS/HR)

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Project description:Rhizosphere soil microbial sequencing of Panax notoginseng

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iTRAQ results of recalcitrant Panax notoginseng seeds

Project description:Panax notoginseng (Burk) F.H. Chen is an importantly economical and medicinal plant of the family Araliacease, and its seeds are obviously characterized by the recalcitrance and after-ripening process. an isobaric tag for relative and absolute quantification (iTRAQ) and RNA-seq was used to analyze the proteomic changes and transcriptomic in seeds of P. notoginseng during the after-ripening process .

2021-09-15 | PXD028471 |

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