Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA111501 | ENA |
REPOSITORIES: ENA
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Project description:Proteome analysis of Lung tissue of mice bearing B16-F10-luc-G5 melanoma tumor with sleep fragmentation and with or with out the asdmistration of GL-pp. The mice were randomly divided into 4 groups: control group in general condition with no further treatment (CON group), tumor group with the burden of B16-F10-luc-G5 cells (Tumor group), T+SF group with SF and the burden of B16-F10-luc-G5 cells (T+SF group), and GL-pp group with SF, tumor cells burden, and the administration of 80 mg/kg GL-pp (GL-pp group). B16-F10-luc-G5 cells (5 × 1000000 cells/100 µL per mouse) were injected into the mice through the tail vein. The lung tissue of T+SF group and GL-pp group were analyzed by the proteome.
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Project description:The CCL5/CCR5 axis plays a pivotal role in tumor progression and metastasis. We previously reported CCR5 promotes melanoma EMT and metastasis by upregulating TGFβ1 expression via the PI3K/AKT/GSK3β pathway. However, the full spectrum of downstream events triggered by CCR5 activation remains poorly understood. Here we employed quantitative phosphoproteomics to profile dynamic phosphorylation events in B16/F10 melanoma cells following CCL5 stimulation at three time points (5, 10 and 30 min). Temporal analysis revealed that CCL5 treatment modulated 256, 134, and 83 phosphosites at these respective time points, with a predominant bias toward upregulation. In total, 393 phosphosites across 315 phosphoproteins exhibited significant regulation. To specifically dissect CCR5-mediated phosphorylation events (distinct from other CCL5 receptors), we generated CCR5 knockout B16/F10 cells and performed comparative phosphoproteomic analysis against wild type cells at the 5-min CCL5 stimulation timepoint. This approach identified 52 phosphoproteins regulated by the CCL5/CCR5 axis, whose temporal phosphorylation dynamics were illustrated through heatmap visualization. Gene Ontology (GO) enrichment analysis revealed that CCL5/CCR5 axis participates in various biological processes, including gene expression, cell cycle, DNA repair and cytoskeleton organization. Notably, the phosphorylation of three cell cycle- associated proteins: Cep131, Khdrbs1 and Mak6 was analyzed in detail. All three proteins exhibited transient phosphorylation induction within 5-15 min of CCL5 stimulation, followed by attenuation at 15-30 min, and the phosphorylation activation was dependent on the CCR5. Overall. these findings provide a comprehensive phosphorylation resource for dissecting the molecular mechanisms underlying CCL5/CCR5-facilitated tumor progression, and provide important cues for identifying potential therapeutic targets for melanoma or other related diseases.
2026-07-14 | PXD066097 | Pride
Project description:Expression data of Met-low and Met-high B16-F10 malignant melanoma cells
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Project description:RNA-Seq of B16-F10 mouse melanoma cell line and gene expression profiling
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Project description:To investigate the impact of Card11 on TIL differentiation, we isolated TILs from B16-F10 tumors of WT mice, E134G mice, and K215M mice and performed scRNA seq. We also sorted tumor-infiltrating lymphocytes (TILs) from melanoma(B16-F10) of WT mice and conducted scRNA and scTCR sequencing. To further investigate the impact of Card11 on TCR clonal diversity, we sorted CD8 T cells from melanoma WT mice or K215M mice and performed scRNA and scTCR sequencing.
2025-04-23 | GSE292543 | GEO
Project description:Analysis of gene expression profile of B16-F10 murine melanoma cells exposed to hypoxic conditions (1% oxygen) or hypoxia mimicry (cobalt chloride) for 24 hours. Gene expression profiles were analyzed using MG-U74Av2 oligonucleotide microarrays. Data analysis revealed 2541 probesets (FDR<5%) for 1% oxygen experiment and 364 probesets (FDR<5%) for cobalt chloride, that showed differences in expression levels. Analysis of hypoxia-regulated genes (1% O2) by stringent Family-Wise Error Rate estimation indicated 454 significantly changed transcripts (p<0.05). The most upregulated genes were Lgals3, Selenbp1, Nppb (more than ten-fold increase). Both hypoxia and hypoxia-mimicry induced HIF-1 regulated genes. However, unsupervised analysis (Singular Value Decomposition) revealed distinct differences between gene expression induced by these two experimental conditions. We investigated transcriptional activity of B16-F10 murine melanoma cells cultured for 24h under hypoxic (nominal 1% oxygen; 9 experimental samples and 6 controls) and hypoxia-mimicking conditions (cobalt chloride, 100 M-NM-<M or 200 M-NM-<M, 2 samples each and 2 controls).
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Project description:B16-F10 malignant mouse melanoma cells have been frequently used as highly metastatic cells. Based on heterogenous cell surface expression of Met/HGF (hepatocyte growth factor) receptor in B16-F10 cells, the cells were divided into Met-low and Met-high cells by flow cytometry and these populations were subjected to microarray analysis. Met-low and Met-high cells showed different expression profiles in genes involved characteristics of tumors, including stem cell maintenance, pigmentation, and angiogenesis.
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Project description:The effect of TIM-3 stimulation was studied on B16F10 mouse melanoma cells, in vitro.
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