Project description:Polyhydroxyalkanoates (PHAs) production using cellulosic biomass is a promising way for sustainable manufacturing of bioplastics. The famous bacterium, Priestia megaterium, is an ideal choice because it can utilize glucose and xylose for PHA synthesis. In the present study, we engineered the genome of P. megaterium by CRISPR-Cas9 system to enhance cellobiose utilization and PHA production. The genes encoding for β-glucosidases (Bgls) from different microbes were introduced into the P. megaterium to improve the cellobiose utilization.
