Rattus norvegicus
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ABSTRACT:
PROVIDER: PRJNA111555 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Diet induced gene expression in rat peripheral blood mononuclear cells
| PRJNA112525 | ENA
Project description:Expression data from rat peripheral blood mononuclear cells (PBMCs)
| PRJNA336375 | ENA
Project description:This study analyzed gene expression of rat peripheral blood mononuclear cells by microarray analysis following different feeding conditions (ad libitum feeding, fasting and refeeding) in normoweight (control) and in diet-induced obese rats (cafeteria rats). The aim of this study was to identify genes and biological pathways that were altered by feeding conditions in normoweight and diet-induced obese rats. Keywords: Dietary treatment, analysis of feeding conditions
2009-12-31 | GSE14497 | GEO
Project description:Gene expression of rat peripheral blood mononuclear cells was analyzed by microarray analysis in normoweight and in diet-induced obese rats (cafeteria rats). The aim of this study was to identify genes involved in energy homeostasis that are altered in the obese state. Keywords: Dietary treatment, obese-state analysis This study analyzed the gene expression of PBMC in normoweight and in diet-induced obese (cafeteria-fed) Wistar rats submitted to ad libitum feed conditions. Two-month-old male Wistar rats (n=10) were assigned into two dietary groups for 4 months: the control group (n=5) was fed with a standard chow diet, whereas the second group (cafeteria group, n=5) was fed with a fat-rich hypercaloric cafeteria diet in addition to the standard chow. At 6 months of age, blood samples were collected in feeding conditions from the safena vein. Peripheral blood mononuclear cells were isolated by Ficoll gradient separation, and RNA was extracted. One sample in the control group was excluded because of a low amount of RNA. Gene expression changes were assessed using an Agilent rat whole genome microarray (G4131F Agilent Technologies).
2009-12-01 | E-GEOD-14094 | biostudies-arrayexpress
Project description:Gene expression of rat peripheral blood mononuclear cells was analyzed by microarray analysis in normoweight and in diet-induced obese rats (cafeteria rats). The aim of this study was to identify genes involved in energy homeostasis that are altered in the obese state. Keywords: Dietary treatment, obese-state analysis
2009-12-01 | GSE14094 | GEO
Project description:Transcriptional gene profile of peripheral blood derived mesenchymal stromal cells from GFP transgenic rat
| PRJNA106691 | ENA
Project description:Detailing Novel Epigenetic Events Associated with Diet-Induced Obesity in the Rat
| PRJNA391424 | ENA
Project description:Comparison of radiation-induced rat mammary carcinomas from individuals with different sensitivity to diet-induced obesity
| PRJNA292703 | ENA
Project description:Knee osteoarthritis (KOA), as a degenerative multifactorial disease, affects the quality of life and mental health of patients, and also brings a huge socioeconomic burden. Treating synovitis have shown promise as anti-inflammatory therapeutics in mitigating OA symptoms and disease progression. Here, by analysing synovial single-cell sequencing (scRNA-seq) data from KOA, we found that synovial fibroblasts (FLS) in OA synovium showed a distinct pro-inflammatory phenotype. We collected synovial tissue from patients with clinical OA as well as from healthy donors, and histological examination was consistent with findings in scRNA-seq. Inspired by recent cross-tissue fibroblast lineage studies, we identified by sequencing that healthy FLS in synovial tissues share transcriptome-level similarities with dermal fibroblasts (DFb). Subsequently, we revealed the local as well as systemic distribution of intra-articular injected DFbs by constructing/extracting two types of rat fibroblasts (luciferase DFbs as well as GFP DFbs). The results demonstrate that DFbs can be locally retained in the synovium for up to three weeks following targeted engrafting on it. And intra-articular injection does not result in DFbs migration to vital organs or the occurrence of histological changes in these organs. A rat model of KOA was constructed by anterior cruciate ligament transection (ACLT) in order to study the therapeutic effect of DFbs on KOA. After injection, the rats showed improvement in painful gait. In addition, histological as well as imaging results showed reduced synovitis and improvement in articular cartilage. Finally we verified the protective effect of DFbs on cytokine-stimulated chondrocytes in a co-culture system.
2026-06-24 | GSE304724 | GEO