Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA111781 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene expression profiling of retrograde PAP-signaling and ABA-signaling mutants in response to ABA treatment
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Project description:Expression data from Arabidopsis gapcp mutant treated with ABA
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Project description:Comparative transcriptome analysis between wild-type and gpa1 mutant in response to ABA
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Project description:Although abscisic acid (ABA) and gibberellins (GAs) play pivotal roles in many physiological processes in plants, their interaction in the control of leaf growth remains elusive. In this study, genetic analyses of ABA and GA interplay in leaf growth were performed in Arabidopsis thaliana. The results indicate that for ABA and GA interaction, leaf growth of both the aba2/ga20ox1 and aba2/GA20OX1-OE plants exhibits partially additive effects but is similar to the aba2 mutant. Consistent with this result, transcriptome analysis suggests that a substantial proportion (45-65%) of the gene expression profile of aba2/ga20ox1 and aba2/GA20OX1-OE plants overlaps and shares a similar pattern to the aba2 mutant. Thus, these data support that ABA deficiency dominates leaf growth regardless of GA levels. Moreover, gene ontology (GO) analysis indicates gene enrichment in the categories of hormone response, developmental and metabolic processes, and cell wall organization in these three genotypes. Leaf developmental genes are also involved in ABA-GA interaction. Collectively, these data support that the genetic relationship of ABA and GA interaction involves multiple coordinated pathways rather than a simple linear pathway in the regulation of leaf growth. To better understand the molecular basis of ABA and GA interaction, transcriptome analysis was performed among the genotypes used in this study.
2015-06-01 | GSE61344 | GEO
Project description:Expression data from stratified anac089-2 mutant and wild-type seeds, ABA-treated.
| PRJNA134349 | ENA
Project description:Expression data from stratified mads-box (agl67) mutant and wild-type seeds, ABA-treated
| PRJNA133815 | ENA
Project description:The role of the highly histone variant H2A.Z remains engimatic, in part because of its context-dependent effects on modulating transcription. Here, we investigate the role of the H2A.Z N-terminal tail in regulating transcriptional responses to the stress hormone abscisic acid (ABA) in Arabidopsis thaliana. In WT plants, treatment with ABA induces hundreds of transcripts to be rapidly up- and downregulated. Using h2a.z null mutant plants expressing either an H2A.Z missing the entire N-temrinal tail (Delta28), or carrying lysine to arginine mutations in either a single lysine (K>R 3) or all nine N-terminal lysines (K>R 1-9), we find that the H2A.Z N-terminus is surprisingly dispensable for the intiation of the ABA response program, but plays an important role in fine-tuning the magnitude of response. Interestingly, distinct subsets of genes exhibit altered response magnitude in each mutant, with limited overlap between genotypes. The most significantly dysregulated of these in the mutants compared to WT are associated with chromatin context: genes upregulated by ABA are enriched for gene-body H2A.Z (gbH2A.Z) at baseline, and show increased expression in mutants primarily under mock (non-ABA) conditions. Meanwhile, ABA-repressed genes tend to be enriched for transcription start site (TSS)-localized H2A.Z at baseline, and exhibit enhanced repression in mutants upon ABA treatment. Notably, the K>R 1-9 mutant produces more differentially expressed genes (DEGs) compared to WT in both mock and ABA-treated conditions that does complete removal of the tail, indiciating that loss of modifiability is not equivalent to loss of the N-terminus. Together, our results support a model in which the major regulatory functions of H2A.Z reside in the highly conserved histone fold domain, while the more evolutionarily variable N-terminal tail acts as a bidirectional buffer to fine-tune transcirptional output. While dispensable for the broad execution of transcriptional programs, this fine-tuning capacity is critical for maintaining transcriptional precision during stress responses, with implications for organismal fitness.
2026-07-24 | GSE338700 | GEO
Project description:High salinity is one of the major environmental factors, which hampers plant growth, development and productivity. To better understand the regulatory mechanisms by which plants cope with salt stress, we used genetic approaches to identify salt hypersensitive mutant 9 (sahy9), a new allele of apum23, in Arabidopsis thaliana. The sahy9/apum23 mutant seedlings display postgemination developmental arrest and later become bleached under agar plates supplemented with various salt stressors. Transcriptomic and proteomic analyses of the salt-treated sahy9/apum23 and wild-type seedlings revealed differential expression of genes with similar functional categories, primarily including cellular and metabolic processes, and abiotic and biotic stress responses. However, the consistency of gene expression at both transcript and protein levels is low (), suggesting the involvement of posttranscriptional processing in salt response. Furthermore, the altered gene/protein expression mediated by SAHY9/APUM23 in salt sensitivity is involved in several functional groups, particularly in ABA biosynthesis and signaling, abiotic stress response, LEA proteins, and ribosome biogenesis-related genes. Importantly, NCED3, a key gene involved in ABA biosynthesis, and major ABA responsive marker genes, such as RD20 and RD29B, are down-regulated at both transcript and protein levels in sahy9/apum23 under salt stress. Consistently, lower contents of ABA and proline, and expression changes of a subset of LEA proteins also support the nature of sahy9/apum23 showing salt hypersensitivity. Collectively, these data suggest that SAHY9/APUM23-mediated salt response is associated with ABA signaling pathway and its downstream stress responsive or tolerant genes.
2018-02-20 | GSE99664 | GEO
Project description:Transcriptional response of wild type ABA-treated Arabidopsis seedlings
| PRJNA101539 | ENA
Project description:Analysis of gene expression in hda19 mutant in response to shoot induction
| PRJDB13418 | ENA