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ABSTRACT:
PROVIDER: PRJNA1122086 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes.
2013-12-31 | GSE53563 | GEO
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes.
2013-12-31 | GSE53562 | GEO
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes. Transcriptional profiling variation of S. coelicolor M145, M-NM-^TscbR2 mutation with jadomycin addition at 30 h.
2013-12-31 | E-GEOD-53563 | biostudies-arrayexpress
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes. Transcriptional profiling of S. coelicolor M145 at different growth phases (exponential, transitional and stationary phase)
2013-12-31 | E-GEOD-53562 | biostudies-arrayexpress
Project description:We sequenced total RNA from whole blood samples of 27 wild gray wolves from Yellowstone National Park.
2016-07-27 | GSE80440 | GEO
Project description:Genomes of streptomycetes isolated from beehives
| PRJNA746445 | ENA
Project description:UV-crosslinked mouse intestine was immunoprecipitated for MSI1. Similar CLIP protocol was performed in Park et al. 2015 Journal of Experimental Medicine except RNA was random primed and hybridized to Affymetrix arrays 1.0ST array. Fold enrichment was ranked over IgG.
2017-04-24 | GSE74694 | GEO
Project description:Streptomycetes sp. LZZY-S3 isolate:soil Genome sequencing
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