Streptomyces
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA631977 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes.
2013-12-31 | GSE53562 | GEO
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes.
2013-12-31 | GSE53563 | GEO
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes. Transcriptional profiling of S. coelicolor M145 at different growth phases (exponential, transitional and stationary phase)
2013-12-31 | E-GEOD-53562 | biostudies-arrayexpress
Project description:Genomes of streptomycetes isolated from beehives
| PRJNA746445 | ENA
Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes. Transcriptional profiling variation of S. coelicolor M145, M-NM-^TscbR2 mutation with jadomycin addition at 30 h.
2013-12-31 | E-GEOD-53563 | biostudies-arrayexpress
Project description:Plant-associated streptomycetes are persistent in plant microbiomes, yet how they cope with pathogen-derived toxins remains poorly understood. Fusarium species produce fusaric acid (FA), a mycotoxin that contributes to virulence and perturbs plant-associated microbial communities. Here, we show that the Arabidopsis endophyte Streptomyces sp. ATMOS43 exhibits characteristic tolerance to FA. Metabolomics analysis revealed the formation of single amino acid and dipeptidyl conjugates of FA, including FA-Ser, a major conjugate that lacked detectable toxicity in bacterial and plant assays. Co-cultivation experiments showed that FA detoxification by ATMOS43 restores growth of sensitive streptomycetes in the presence of FA. This activity is enriched among plant-associated isolates, suggesting adaptation to toxin-rich environments. Proteomics and physiological analyses further indicate FA toxicity involves zinc chelation, which is abolished upon conjugation to FA-Ser, although additional cellular targets may also contribute. Together, our findings show that streptomycetes can protect plants not only by inhibiting pathogens themselves, but also by neutralizing pathogen-derived toxins.
2026-08-03 | PXD082028 | Pride
Project description:Salinity stress-induced metabolic reprogramming in Streptomycetes
| PRJNA1315997 | ENA
Project description:The Streptomyces lividans lsp gene encodes a type II signal peptidase (Lsp) that cleaves the type II leader peptides of lipoproteins. Transcriptional profiling of the bacterium depleted of the lsp gene mainly resulted in deactivation of the sigma U regulon, as well as in downregulation of genes involved in the biogenesis and function of ribosomes and genes encoding some major secretory proteins as determined by hybridisation of commercially available S. lividans genome-wide microarrays. Almost 50% of the dowregulated genes have been described as forming part of the stringent response in streptomycetes. The gene encoding the S. lividans extracellular foldase, the lipoprotein FkpA, is equally downregulated. Therefore, the deletion of lsp from the S. livdans genome temporarily triggers a cellular stress where the stringent response is, at least, partially induced.
2014-05-22 | GSE30728 | GEO