Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comprehensive analysis of the secretome and transcriptome stability from WJ-Mesenchymal Stromal cells throughout GMP-Manufacturing

ABSTRACT: Comprehensive analysis of the secretome and transcriptome stability from WJ-Mesenchymal Stromal cells throughout GMP-Manufacturing

PROVIDER: PRJNA1122573 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29362218.fastq.gz	Fastqsanger.gz
	SRR29362219.fastq.gz	Fastqsanger.gz
	SRR29362220.fastq.gz	Fastqsanger.gz
	SRR29362221.fastq.gz	Fastqsanger.gz
	SRR29362222.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 69

Similar Datasets

Comprehensive analysis of the secretome and transcriptome stability from WJ-Mesenchymal Stromal cells throughout GMP-Manufacturing

Project description:Mesenchymal stromal cells (MSCs) hold promise for cell-based therapies due to their ability to stimulate tissue repair and modulate immune responses. Umbilical cord-derived MSC from Wharton’s jelly (WJ), offer advantages such as low immunogenicity and potent immune modulatory effects. However, ensuring consistent quality and safety throughout their manufacturing process remains critical. RNA sequencing (RNA-seq) emerges as a crucial tool for assessing genetic stability and expression dynamics. This study examines the secretome and transcriptome signatures throughout WJ-MSCs Good Manufacturing Practice (GMP) production, focusing in the role of Total RNA or Massive Analysis of cDNA Ends (MACE-Seq)-RNA sequencing. Through comprehensive transcriptomic analysis, we demonstrated the stability of WJ-MSC transcriptome across manufacturing stages. Notably, MACE-Seq enhanced the identification of key expression patterns related to senescence and immunomodulation. These findings highlight the potential of MACE-Seq as a routine quality assessment tool for WJ-MSC-based therapies, ensuring their efficacy and safety in clinical applications. Importantly, MACE-Seq proves valuable for characterizing WJ-MSC products, providing insights unattainable through traditional assays.

2024-06-30 | GSE269586 | GEO

Streptococcus suis strain:K73-WJ | isolate:K73-WJ

Project description:Streptococcus suis strain:K73-WJ | isolate:K73-WJ Genome sequencing

| PRJNA695389 | ENA

Streptococcus suis strain:K56-WJ | isolate:K56-WJ

Project description:Streptococcus suis strain:K56-WJ | isolate:K56-WJ Genome sequencing and assembly

| PRJNA694778 | ENA

Epigenetic Effects of Maternal Obesity on Wharton's Jelly (WJ) Mesenchymal Stromal Cells (MSCs): Implications For Cellular Therapy. (expression study)

Project description:7 WJ-MSC lines from non-obese BMI <25 kg/m2 were compared with 7 WJ-MSC lines obese BMI >30 kg/m2. No template was used as a negative control Triplicates for each line were analyses for human whole-genome gene expression profile using HumanHT-12 v4.0 Expression BeadChip (Illumina).

2017-11-21 | GSE107214 | GEO

Epigenetic Effects of Maternal Obesity on Wharton's Jelly (WJ) Mesenchymal Stromal Cells (MSCs): Implications For Cellular Therapy. (methylation study)

Project description:7 WJ-MSC lines from non-obese BMI <25 kg/m2 were compared with 7 WJ-MSC lines obese BMI >30 kg/m2. No template was used as a negative control Triplicates for each line were analyses for epigenome-wide methylation (>480,000 CpG sites) using Infinium Human Methylation 450 BeadChip Kit (Illumina).

2016-10-16 | GSE74167 | GEO

Manufacturing Differences Affect Human Bone Marrow Stromal Cell Characteristics and Function: Comparison of Production Methods and Products from Multiple Centers

Project description:Human bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) are manufactured using many different methods, but little is known about the spectrum of manufacturing methods used and their effects on BMSC characteristics and function. Seven centers using, and one developing, Good Manufacturing Practices (GMP) processes were surveyed as to their production methods. Among the seven centers, all used marrow aspirates as the starting material, but no two centers used the same manufacturing methods. Two to four BMSC lots from each center were compared using global gene expression. Among the twenty-four BMSC lots from the eight centers intra-center transcriptome variability was low and similar among centers. Principal component and Unsupervised Hierarchical Clustering analysis separated all the lots from five centers into five distinct clusters. BMSCs from six of the eight centers were tested for their ability to form bone and support hematopoiesis by in vivo transplantation. Those from all six centers tested formed bone, but the quantity formed was variable highly and BMSCs from only three centers supported hematopoiesis. These results show that differences in manufacturing resulted in variable BMSC characteristics including their ability to form bone and support hematopoiesis.

2017-05-15 | GSE92640 | GEO

Melanoleuca sp. WJ-2024a

Project description:Melanoleuca sp. WJ-2024a Genome sequencing and assembly

| PRJNA1201806 | ENA

A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa

Project description:Gene editing in induced pluripotent stem (iPS) cells has been hailed for enabling new cell therapies for various monogenetic diseases including dystrophic epidermolysis bullosa(DEB). However, manufacturing, efficacy, and safety roadblocks have limited the development of genetically corrected, autologous iPS cell-based therapies. We developed Dystrophic Epidermolysis Bullosa Cell Therapy (DEBCT), a new generation GMP-compatible (cGMP), reproducible, and scalable platform to produce autologous clinical-grade iPS cell-derived organotypic induced skin composite (iSC) grafts to treat incurable wounds of patients lacking type VII collagen (C7).

2024-06-30 | GSE229920 | GEO

EP - GMP

Project description:Mouse erythroid progenitors (EP) in comparison to granulocyte/monocyte - macrophage progenitors (GMP) from 10 - 16 week old C57/Bl6 - S129Ola (mixed genetic background) purified by flow cytometry Keywords = EP Keywords = GMP Keywords = hematopoiesis Keywords: other

2004-10-22 | GSE1584 | GEO

Tilletia setariae strain:WJ-1

Project description:Tilletia setariae strain:WJ-1 Raw sequence reads

| PRJNA841014 | ENA