Project description:Photoperiod is a key environmental factor regulating circadian rhythm and physiology in aquatic organisms, but its effects on the immune function of the sea urchin Strongylocentrotus intermedius (S. intermedius) remain unclear. This study investigated photoperiodic regulation of immune resistance, transcriptomes, and circadian rhythm in S. intermedius, and the role of circadian clock gene Cryptochrome1 (Cry1) in immunity. The sea urchins were acclimated to four photoperiods (LL, DD, LD, DL) for one month, followed by Pseudoalteromonas sp. challenge. Bacterial load and gene expression were detected by qRT-PCR; transcriptomic analysis identified DEGs between LD-ZT0 and LD-ZT12, and Cry1 was knocked down via dsRNA for verification. LD photoperiod significantly enhanced antibacterial resistance, with the lowest bacterial load and highest expression of immune genes (SOD, CAT, TNFSF14, C3, ACP, NLRP3) at 12/24 hpi. Transcriptome analysis identified 1450 DEGs enriched in immune (innate immunity, NLRP3 inflammasome assembly) and circadian pathways. LD maintained robust 24-hour rhythms of circadian (Cry1, Cycle, Clock, Rev-erbα) and immune genes, while DL induced phase inversion and LL/DD abolished rhythms. Cry1 knockdown reduced bacterial load and upregulated NLRP3, C3, IL-17D, and TNFSF14. Collectively, LD is optimal for S. intermedius circadian rhythm and immune homeostasis, and Cry1 negatively regulates its immune response. This study provides insights into photoperiodic regulation of echinoderm immunity and support for S. intermedius aquaculture. Collectively, these findings indicate that LD photoperiod is optimal for maintaining circadian rhythm and immune homeostasis of S. intermedius, and Cry1 plays a negative regulatory role in its immune response. This study provides novel insights into the molecular mechanisms of photoperiod regulating immune function in echinoderms and theoretical support for aquaculture management of S. intermedius.
Project description:we generated new reference genome assemblies for two species belonging to two different echinoderm classes: the bat sea star Patiria miniata and the purple sea urchin Strongylocentrotus purpuratus
Project description:Transcriptional profiling of purple sea urchin (Strongylocentrotus purpuratus) larvae cultured under three different seawater CO2 concentrations 400, 800, 1200 µatm. The goal was to determine the effects of CO2, an important climate change variable, on global gene expression
2013-01-01 | GSE37522 | GEO
Project description:Transcriptome profiling of the sea urchin Strongylocentrotus intermedius with different PUFA
| PRJNA759708 | ENA
Project description:Transcriptome profiling of the sea urchin Strongylocentrotus intermedius during gonad development
Project description:Transcriptional profiling of purple sea urchin (Strongylocentrotus purpuratus) larvae cultured under four different seawater conditions: (i)13°C/400 µatm pCO2, (ii)13°C/1100 µatm pCO2, (iii)18°C/400 µatm pCO2 (iv)18°C/1100 µatm pCO2. The goal was to determine the effects of temperature and CO2, both important climate change variables, on gene expression
Project description:Runt domain (Runx) transcription factors constitute a metazoan family of sequence specific DNA binding proteins that are essential for animal development. The sea urchin Runx gene SpRunt-1, which is expressed globally during early embryogenesis, is required for blastula stage cell proliferation and subsequently for cell survival. To obtain a comprehensive list of SpRunt-1 regulatory targets we screened a custom microarray representing the blastula stage transcriptome of Strongylocentrotus purpuratus, comparing relative hybridization signals generated by labeled RNA from 18 hr control versus SpRunt-1 morphant embryos.
Project description:Transcriptional profiling of purple sea urchin (Strongylocentrotus purpuratus) larvae cultured under three different seawater CO2 concentrations 400, 800, 1200 M-BM-5atm. The goal was to determine the effects of CO2, an important climate change variable, on global gene expression Larvae were cultured under three different seawater CO2 concentrations 400, 800, 1200 M-BM-5atm, each with four replicate cultures, and sampled at two developmental stages (gastrula and pluteus)
