Rejuvenation of aged oocyte through exposure to young follicular microenvironment
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1124859 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR29432346_1.fastq.gz | Fastqsanger.gz | |||
| SRR29432346_2.fastq.gz | Fastqsanger.gz | |||
| SRR29432347_1.fastq.gz | Fastqsanger.gz | |||
| SRR29432347_2.fastq.gz | Fastqsanger.gz | |||
| SRR29432348_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 90 |
Similar Datasets
Project description:Reproductive aging is a major cause of fertility decline, attributed to decreased oocyte quantity and competence. Follicular somatic cells play crucial roles in the growth and development of the oocyte by providing nutrients and regulatory factors. Here we investigated how oocyte quality is affected by its somatic cell environment by creating chimeric follicles, whereby an oocyte from one follicle was transplanted into and cultured within another follicle whose native oocyte was removed. Somatic cells within the chimeric follicle re-establish connections with the oocyte and support oocyte growth and maturation in a three-dimensional (3D) culture system. We show that young oocytes transplanted into aged follicles exhibited reduced meiotic maturation and developmental potential, whereas the young follicular environment significantly improved the rates of maturation, blastocyst formation and live birth of aged oocytes. Aged oocytes cultured within young follicles exhibited enhanced interaction with somatic cells, more youth-like transcriptome, remodelled metabolome, improved mitochondrial function, and enhanced fidelity of meiotic chromosome segregation. These findings provide the basis for a future follicular somatic cell-based therapy to treat age-associated female infertility.
2024-07-22 | GSE270016 | GEO
Project description:While the rejuvenation of aged human organs remains one of the most ambitious goals in medicine, it is known that tissue aging can be modulated by circulating systemic factors. Previously, we had shown that transplantation of aged human skin onto immunocompromised mice results in epidermal rejuvenation. However, the underlying mechanisms remained unknown. Therefore, to disclose such mechanisms, aged human skin xenotransplants were analyzed transplated in SCID/Beige young mice and analyzed by RNA-Seq in pre-transplanted, one, two and four weeks after transplantation.
2025-09-26 | GSE138249 | GEO
Project description:Our aim with this work is to identify and quantify the miRNA profile of the human follicular fluid using a miRNA microarray approach in young and advanced-aged women and in follicles with different oocyte quality. Data collected in this study would be utilized in a future as a marker to predict the oocyte quality.
2015-05-12 | GSE63737 | GEO
Project description:The purpose of this study are to compare transcriptome profiling (RNA-seq) data in young and aged oocytes,and to evaluate the transcriptome alterataions after rapamycin treatment in aged oocytes. The sequencing libraries from young, aged and rapa-treated oocytes were generated according to protocols of Smart-seq2.Then cDNA libraries were checked on a Bioanalyzer instrument (Agilent) and the passed samples were finally sequenced by Illumina novaseq for 150-bp paired-end reads. RNA-seq data were aligned to the mouse genome mm10 using STAR 2-pass mode with the annotations of Ensembl version 90 and parameter ‘--outFilterMismatchNmax 6’. SAMtools was used to extract uniquely mapped reads with mapping quality more than or equal to 20. The number of reads mapped to each gene was counted using the HTSeq python package with the ‘union’ overlap resolution mode and –stranded = no. The expressions of genes were quantified as RPKM by edgeR . Genes with RPKM more than 1 in at least three control samples were considered as expressed. Only expressed genes were kept for the subsequent analysis. Differentially expressed genes were identified by DESeq2 using two criteria: fold change of expression should be higher than 2 and the adjusted p-value should be lower than 0.05. Enriched GO terms were obtained with P-value less than 0.05 from DAVID.The analysis of data revealed a total of 454 genes were significant differentially expressed during oocyte aging,of which, 242 genes were upregulated and 212 downregulated in aged oocytes.GO enrichment analysis showed that some of gene expressions were related to oxidation-reduction process, cell cycle, translation and mitochondrial functions. However, gene expression analysis revealed only 4 differently expressed genes between oocyte from rapa-treated and reproductively aged mice.
2022-12-31 | GSE163981 | GEO
Project description:The origin and nature of recently discovered age-associated B cells (ABCs) is under investigation. We used a microarray to examine the transcriptional signatures of ABCs, follicular B cells from aged mice (AFO), and follicular B cells from young mice (YFO).
2016-05-20 | GSE81650 | GEO
Project description:Our aim with this work is to identify and quantify the miRNA profile of the human follicular fluid using a miRNA microarray approach in young and advanced-aged women and in follicles with different oocyte quality. Data collected in this study would be utilized in a future as a marker to predict the oocyte quality. This observational prospective study includes women enrolled in the assisted reproduction program of Hospital Universitari i Politècnic la Fe (Valencia, Spain). The study was accepted by IRB of this Hospital and all couples received informed consent of our study and assisted technique according to service protocols. The study was divided in two stages: Stage 1: Patients (n=21) who had follicles yielding oocytes with different degree of maturation were included (n=21). The follicular fluid samples from 21 patients were pooled in different groups. Expression of miRNA in the follicular fluids was compared within each patient according the degree of oocyte maturation. Stage 2: Patients were allocated into two groups according to the patient's age: advanced age (AA; >36 years old) and young age (YA; <36 years old). Follicular fluids from follicles containing MII oocytes were pooled and miRNA expression was subsequently compared. Please note that the MII samples with underscore (such as MII1 vs MII_1) indicate different pools so they are not the same sample, but we maintained the number because they correspond to paired samples pools for GV and MI.
2015-05-12 | E-GEOD-63737 | biostudies-arrayexpress
Project description:Transcriptome analysis of gene expression in young and aged oocyte samples (RNA-Seq)
| PRJNA688471 | ENA
Project description:Aging is associated with a progressive decline in cellular function. To reset the aged cellular phenotype, various reprogramming approaches, including mechanical routes, have been proposed. However, the epigenetic mechanisms underlying cellular rejuvenation are poorly understood. We studied the transcriptional changes in young, aged and mechanically rejuvenated fibroblasts using RNA-seq. The mechanically rejuvenated aged fibroblasts, that had reset their transcription to a younger cell state. In addition, the rejuvenated cells contractile properties were maintained over multiple cell passages. Taken together, our results provide a multi-scale characterization of the chromatin reorganization that accompanies cellular aging and rejuvenation.
2024-03-20 | GSE237269 | GEO
Project description:Paracrine-Mediated Rejuvenation of Aged Mesenchymal Stem Cells
| PRJNA844080 | ENA