Project description:This SuperSeries is composed of the following subset Series: GSE26617: Saccharomyces cerevisiae bottom of the fermentor vs. feeding GSE26618: Saccharomyces cerevisiae bottom of the fermentor vs. fermentation Refer to individual Series
Project description:Saccharomyces cerevisiae colonies were grown on synthetic minimal media containing 1% glucose. The top layer of cells was scraped off using a custom plastic guide and a cell scraper. The remaining bottom layer of cells was then washed off with water and proteomics samples were prepared from both layers separately. Label-free quantification by DIA-SWATH was used to assess differences in gene expression between top and bottom layers.
2023-02-13 | PXD033395 | Pride
Project description:Saccharomyces cerevisiae bottom of the fermentor vs. feeding and fermentation
Project description:This study investigated the impact of simulated microgravity (SMG) on SUMOylation and protein expression in Saccharomyces cerevisiae. Utilizing a rotating wall vessel to simulate microgravity conditions, the research employed SILAC labeling and bottom-up proteomics to differentiate gravity vs SMG for SUMOylation (exp 1742) and for protein expression (exp 1772). N=6 for each experiment.
Project description:The homologous Ace2 and Swi5 transcription factors of Saccharomyces cerevisiae have identical DNA-binding domains, and both are cell cycle regulated. There are common target genes, as well as genes activated only by Ace2 and other genes activated only by Swi5. Keywords: genetic modification