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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[Ribotag]

ABSTRACT: B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[Ribotag]

PROVIDER: PRJNA1125426 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29462012_1.fastq.gz	Fastqsanger.gz
	SRR29462012_2.fastq.gz	Fastqsanger.gz
	SRR29462013_1.fastq.gz	Fastqsanger.gz
	SRR29462013_2.fastq.gz	Fastqsanger.gz
	SRR29462014_1.fastq.gz	Fastqsanger.gz

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[Ribotag]

Project description:The establishment of long-lasting immunity against pathogens is facilitated by the germinal center (GC) reaction, during which B cells increase their antibody affinity, and differentiate into antibody-secreting cells (ASC) and memory cells. These events involve modifications in chromatin packaging that orchestrate the profound restructuring of gene expression networks that determine cell fate. While several chromatin remodelers were implicated in lymphocyte functions, less is known about SMARCA5. Here, by using ribosomal pull-down for analyzing translated genes in GC B cells, coupled with functional experiments in mice, we identified SMARACA5 as a key chromatin remodeler in B cells. While the naive B cell compartment and early B cell activation remained unaffected following conditional depletion of Smarca5, immunoglobulin class switching, GC formation and ASC differentiation were impaired. Single-cell multi-omic sequencing analyses revealed that SMARCA5 is crucial for facilitating the transcriptional modifications and genomic accessibility of genes that support B cell differentiation. These findings offer novel insights into SMARCA5, which can be targeted in lymphoma patients.

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[RNA-Seq]

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[CUT & RUN]

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[scMultiome - RNA + ATAC]

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling

Project description:B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[CUT & RUN]

Project description:B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[CUT & RUN]

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[RNA-Seq]

Project description:B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[RNA-Seq]

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[scMultiome - RNA + ATAC]

Project description:B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling[scMultiome - RNA + ATAC]

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Human SMARCA5 interactome in transgenic SMARCA5 mouse model

Project description:We hypothesize that the ISWI ATPase Smarca5 plays a role in hematopoietic stem cell development via its interaction partners, and to study the role, we used a mouse model with transgenic expression of SMARCA5 coupled with mass spectrometry analysis. The study uses transgenic SMARCA5 mouse model with conditional hypomorphic allele (lower expression on protein level compared to controls) incorporated into Rosa26 locus, transgenic allele uses endogenous Rosa26 promoter. The transgenic human SMARCA5 protein has a FLAG tag at its C-terminus, which allows specific immunoprecipitation and also differentiation from the endogenous protein (human and mouse SMARCA5 are almost identical at the protein level). To ensure the highest possible expression of the transgenic allele, we used a model that also had one endogenous Smarca5 allele deleted. Various organs (thymus, spleen, brain, kidney, liver, testes, muscle, fetal liver) were isolated from transgenic mice, lysed and then immunoprecipitated using anti-FLAG antibody. The immunoprecipitates were then analyzed using mass spectrometry to determine SMARCA5 interactomes in various tissues. Transgenic human SMARCA5 was confirmed to form all the expected complexes and capable of replacing the functions of the mouse protein. For details, see the publication of Turkova et al. 2024 in Communications Biology.

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B cell differentiation into germinal center and antibody-secreting cells depends on SMARCA5-mediated chromatin remodeling

Project description:This SuperSeries is composed of the SubSeries listed below.

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