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RNA-seq of worker antennae of honeybees (Apis mellifera) with a dsx stop/stop mutation

ABSTRACT: RNA-seq of worker antennae of honeybees (Apis mellifera) with a dsx stop/stop mutation

PROVIDER: PRJNA1125437 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29460235.fastq.gz	Fastqsanger.gz
	SRR29460236.fastq.gz	Fastqsanger.gz
	SRR29460237.fastq.gz	Fastqsanger.gz
	SRR29460238.fastq.gz	Fastqsanger.gz
	SRR29460239.fastq.gz	Fastqsanger.gz

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RNA-seq of worker antennae of honeybees (Apis mellifera) with a dsx stop/stop mutation

Project description:Transcriptional profiling of the antennae of adult honeybee workers with a dsx stop/stop mutation and wild-type workers was performed by RNA-Seq. Gene expression of the dsx stop/stop and wild type female workers was compared.

2024-07-10 | GSE270222 | GEO

Transcriptome analysis of Apis mellifera antennae reveals molecular divergence underlying the division of labor in worker bees

Project description:Olfaction system plays a fundamental role in mediating insect behavior. Besides, the division of queen, worker and drone, honeybee also exhibit an age-dependent division of labor. Worker bees perform discrete sets of behaviors throughout their lifespan. These behavioral states rely on the sense of the environments and chemical communications via their olfactory system - antennae. However, the olfactory adaption mechanism of workers in these processes of behavioral development is still unclear. In this study, we conducted a comprehensive and quantitative analysis of gene expression in Apis mellifera antenna of newly emerged workers, nurses, foragers, and defenders using RNA-seq. We found that antennae tissues continue to develop after transformation from pupae to adult. Additionally, we identified both developmental and labor-division specific expressed genes. We validated the unexpected discovery of major royal jelly protein genes, which are highly and specifically expressed in nurse honeybee workers. We further identified and validated that significant alternative splicing events are also involved in the development and division of labor. These findings provided a comprehensive transcriptome profile and new perspective into the molecular mechanism underlying honeybee division of labor.

2024-10-24 | GSE221246 | GEO

mRNA-Seq of brain tissue from honeybee (Apis mellifera) drones (male) and workers (female)

Project description:Transcriptional profiling of brain tissue of was performed by RNA-SEQ in females and males. The sex specific expressed and spliced genes were examined comparing drone and worker honeybees. Examination of mRNA profiles in heads of male and female honeybees pupae were compared.

2013-03-27 | E-GEOD-45408 | biostudies-arrayexpress

Phenotypic plasticity shapes genome architecture in the honeybee Apis mellifera (RNA-seq)

Project description:Female honeybees are specified as workers or queens based on diet during early development. Workers are essentially sterile with a reduced number of ovarioles and no spermatheca. In the presence of the queen (queen mandibular pheromone) and her brood, worker ovaries are kept in an inactive quiescent state. If the queen is removed, or lost, worker bees are able to sense this change in their environment and their ovaries undergo complete remodeling producing unfertilized haploid eggs that will produce male (drone bees). In this study we analyze gene expression in queen, worker, and laying worker ovaries using RNA-seq and explore differences in the chromatin landscape (focusing on H3K27me3).

2019-09-17 | GSE120561 | GEO

Phenotypic plasticity shapes genome architecture in the honeybee Apis mellifera (ChIP-seq)

Project description:Female honeybees are specified as workers or queens based on diet during early development. Workers are essentially sterile with a reduced number of ovarioles and no spermatheca. In the presence of the queen (queen mandibular pheromone) and her brood, worker ovaries are kept in an inactive quiescent state. If the queen is removed, or lost, worker bees are able to sense this change in their environment and their ovaries undergo complete remodelling producing unfertilised haploid eggs that will produce male (drone bees). In this study we analyse gene expression in queen, worker, and laying worker ovaries using RNA-seq and explore differences in the chromatin landscape (focussing on H3K27me3).

2019-09-17 | GSE120560 | GEO

mRNA-Seq of brain tissue from honeybee (Apis mellifera) drones (male), workers (female) and queens (female).

Project description:Transcriptional profiling of brain tissue of was performed by RNA-SEQ in workers, queens and males. The sex and caste specific expressed and spliced genes were examined comparing drone, worker and queen honeybees.

2013-03-27 | GSE45408 | GEO

Expression differences between worker(female) and drone(male) honey bee antennae

Project description:We have identified a honeybee (Apis mellifera) odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA) from four candidate sex pheromone odorant receptors from the honeybee genome based on their biased expression in drone antennae. Keywords: Tissue Comparison

2007-08-09 | GSE8519 | GEO

Investigating effects of queen exposure to QueenBoost on queen oocytes and hemolymph as well as worker antennae, hemolymph, and hypopharangeal glands

Project description:Queens were exposed to low doses pyriproxyfen (QueenBoost, which has been previously found to increase egg hatching rates and attentiveness of worker progeny to the queen) orally within queen monitoring cages. After treatment, queens were sampled to obtain proteomics data for hemolymph and mature oocytes (dissected from the ovaries), and workers were sampled to obtain pooled samples of antennae, hypopharangeal glands, and hemolymph. Data from worker antennae and hypopharangeal gland samples were acquired from a single plate, data from worker and queen hemolymph samples were acquired from a second plate, and oocyte data were acquired at a later date from a third plate. All samples were randomized within plates. The hemolymph, antennae, and hypopharangeal gland data were searched together (report_JF-all-tissues file) and the oocyte data were searched separately (report_JF-oocytes file). Data were all searched library-free using DIA-NN with default parameters except "unrelated runs" and "MBR" were selected, the neural network classifier was set to "double-pass mode", and protein inference was set to "protein names from FASTA".

2024-07-31 | MSV000095498 | MassIVE

Transcriptome analysis of Apis mellifera antennae reveals molecular divergence underlying the division of labor in worker bees

Project description:Transcriptome analysis of Apis mellifera antennae reveals molecular divergence underlying the division of labor in worker bees

| PRJNA913589 | ENA

Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Clontech_lib_RNA-seq)

Project description:Queen discrimination behavior in the red imported fire ant Solenopsis invicta maintains its two types of societies: colonies with one (monogyne) or many (polygyne) queens, yet the underlying genetic mechanism is poorly understood. This behavior is controlled by two supergene alleles, SB and Sb, with ~600 genes. Polygyne workers, having either the SB/SB or SB/Sb genotype, accept additional SB/Sb queens into their colonies but kill SB/SB queens. While monogyne workers, all SB/SB, reject all additional queens regardless of genotype. Because the SB and Sb alleles do not recombine, it is difficult to determine which genes within the supergene mediate this differential worker behavior. We hypothesized that the alternate worker genotypes sense queens differently because of different patterns of gene expression in their main sensory organ, the antennae. To identify such differentially expressed genes, we sequenced RNA from four biological replicates of pooled antennae from three groups of workers: monogyne SB/SB, polygyne SB/SB, and polygyne SB/Sb. We identified 81 differentially expressed protein coding genes with 14 encoding potential odor metabolism and perception proteins. We focused on the two differentially expressed odorant perception genes: an odorant binding protein SiOBP12 and an odorant receptor SiOR463. We found that the SiOR463 was lost in the Sb-genome. In contrast, the SiOBP12 has an Sb-specific duplication SiOBP12b’, which was expressed in the SB/Sb worker antennae, while both paralogs SiOBP12 and SiOBP12b’ were expressed in the body. This result indicates that SiOBP12b’ has gained an antennal promoter or enhancer and suggests neofunctionalization, perhaps for queen discrimination behavior.

2020-04-10 | GSE126673 | GEO

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