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RNA-seq for the soybean leaves

ABSTRACT: RNA-seq for the soybean leaves

PROVIDER: PRJNA1126157 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR29474092_1.fastq.gz	Fastqsanger.gz
	SRR29474092_2.fastq.gz	Fastqsanger.gz
	SRR29474094_1.fastq.gz	Fastqsanger.gz
	SRR29474094_2.fastq.gz	Fastqsanger.gz

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soybean leaves

Project description:soybean leaves Raw sequence reads

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Comparative transcriptome analysis of manganese transportation in different leaves in soybean under manganese stress

Project description:Manganese (Mn) stress is known to be a major limitation for development of soybean, and legume crop productivity globally. However, very little information is available on the adaptive mechanisms, particularly in the important legume crop soybean (Glycine Max L.), which enable leaves to respond to high-Mn availability. Thus, to elucidate these mechanisms in soybean leaves at molecular level, we used an RNA sequencing approach to investigate transcriptomes of the leaves under Mn-sufficient and Pi-excessive conditions. Our investigation revealed that more genes showed altered expression patterns in old leaf than in young leaf under Mn excess, suggesting that the Mn excess-more-sensitive old leaf required expression change in a larger number of genes to cope with high-Mn stress than the Mn excess-less-sensitive young leaf. The functional classification of differentially expressed genes (DEGs) was examined to gain an understanding of how leaves respond to Mn stress, caused by soil Mn excess. As a result, more DEGs involved in nodulation, detoxification, nutrient/ion transport, transcriptional factors, key metabolic pathways, Mn remobilization and signalling were found in Mn-excessive induced old leaves than in Mn-excessive induced young leaves. Our findings have enabled the identification of molecular processes that play important roles in the acclimation of leaves to Mn excess, ultimately leading to the development of Mn-efficient soybean suitable for Mn-excessive soils.

2022-08-16 | GSE118649 | GEO

RNAseq data of two week-old soybean leaves response to cold stress

Project description:Transcriptome analysis of cold-treated leaves (unifoliates) of soybean seedlings were performed. RNAseq analysis was performed using two lanes on a Illumina HiSeq2000 and sequenced on a 100bp, paired-end run.

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Transcriptomic study of soybean (Glycine max) leaves in response to short-term phosphorus deficiency

Project description:We conducted a genome-wide transcriptomic analysis in soybean leaves treated with a short-term (24 h) Pi-deficiency using RNA sequencing (RNA-seq) technology. Two biological replicates of RNA-seq were included for both Pi-sufficient leaves (PSL) and Pi-deficient leaves (PDL), and therefore a total of four libraries were constructed. Using a 2-fold change and a P-value ≤0.05 as the cut-off for selecting the differentially expressed transcripts, we globally identified short-term Pi-stress responsive genes. Some DEGs potentially involved in Pi sensing, signaling, and homeostasis were up-regulated by Pi deprivation, including five SPX-containing genes. Some DEGs possibly associated with water and nutrient uptake, hormonal and calcium signaling, protein phosphorylation and dephosphorylation, and cell wall modification were affected at the early stage of Pi deprivation. At least thirty-one transcription factor genes belonging to 10 diverse families were found to be responsive to Pi starvation.

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Quantitative proteomics analysis of soybean leaves under low and high phosphate conditions

Project description:Low phosphate (LP) availability is a critical limiting factor affecting soybean production. Soybean plants develop a series of strategies to adapt phosphate limitation condition. To know the underlying molecular mechanisms responsible for LP stress response. A label-free quantification (LFQ) analysis of soybean leaves grown under low and high phosphate condition was performed.

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RNA-seq for the soybean roots

Project description:RNA-seq for the soybean roots

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Transcriptomics Analyses of soybean leaves and roots sample during water-deficit

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RNA-seq for the soybean leaves

Project description:To investigate the molecular mechanism underlying the involvement of GmPAP23 in intracellular phosphorus recycling, an transcriptomic analysis was performed using the mature leaves of WT and OX5 lines grown under P sufficient conditions at R6 stage,the comparison between WT and OX5 lines revealed that a total of 1658 DEGs were identified between WT and OX5 lines, of which 828 genes were upregulated and 830 genes were downregulated

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