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ABSTRACT: RNASeq of resistant and susceptible Helicoverpa zea

PROVIDER: PRJNA1126714 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29492032_1.fastq.gz	Fastqsanger.gz
	SRR29492032_2.fastq.gz	Fastqsanger.gz
	SRR29492033_1.fastq.gz	Fastqsanger.gz
	SRR29492033_2.fastq.gz	Fastqsanger.gz
	SRR29492034_1.fastq.gz	Fastqsanger.gz

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Project description:Differential expression between Bt-resistant and Bt-susceptible Helicoverpa zea using RNAseq

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Genome scale analysis of host-virus mRNA differential expression in Helicoverpa zea insect cells infected by H. armigera baculovirus from early till late infection

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Genome scale analysis of host-virus mRNA differential expression in Helicoverpa zea insect cells infected by H. armigera baculovirus from early till late infection

Project description:A custom microarray, based on deep transcriptome sequencing ((GEO accession number: GSE34418), was used to simultaneously investigate expression of over 24,000 Helicoverpa zea insect transcripts and 134 H. armigera nucleopolyhedrovirus (HearNPV) genes throughout the infection process at 0, 12, 24 and 48 hours post infection A cutom 8x60,000 SurePrint Agilent expression slide (Agilent, Santa Clara, CA) was used to analyzed eight samples, including biological replicates of HaSNPV-infected cultures at 0, 12, 24 and 48 hpi.The microarray probes included probes for 27,400 H. zea sequences that were validated previously (Nguyen et al., 2012. PLOS ONE, 7(5), e36324) and all 134 H. armigera single-capsid nucleopolyhedrovirus (HearNPV) genes (Accession: NC_002654).

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Helicoverpa zea

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Dietary sterols/steroids and the generalist caterpillar Helicoverpa zea: physiology, biochemistry and midgut gene expression

Project description:Sterols are essential nutrients for insects because, in contrast to mammals, no insect (or arthropod for that matter) can synthesize sterols de novo. Cholesterol is the most common sterol in insects, but it is not found in plants in large quantities; plant-feeding insects typically generate their cholesterol by metabolizing phytosterols. However, different plants species can contain different types of phytosterols, and some phytosterols are not readily converted to cholesterol. In this study we examined, using artificial diets containing single sterols, how typical (cholesterol and stigmasterol) and atypical (cholestanol and cholestanone) sterols/steroids affect the performance of a generalist caterpillar (Helicoverpa zea), restricting this analysis to midgut tissue because this is where sterol/steroid absorption occurs, and the midgut is the putative site of dietary sterol/steroid metabolism. In general, H. zea performed best on the cholesterol and stigmasterol treatments; performance was reduced on cholestanol, and was very poor on cholestanone. We compared the transcript profiles of larval guts in response to differentially suitable sterols, using the optimal sterol, cholesterol, as a control, using a two-color reference design microarray experiment. Midgut gene expression patterns differed between the treatments; relative to cholesterol, differences were lowest on the stigmasterol treatment, intermediate on the cholestanol treatment, and greatest on the cholestanone treatment. Transcriptional profiling comparing Helicoverpa zea gut tissue from third instar larvae exposed to four different dietary sterols, namely Cholesterol (CON), Cholestanol (ChStanol), Cholestan-3-one (Ch3one) and Stigmasterol (Stigma). Two-color reference design. Biological replicates: 4 (5 individuals per replicate). 12 samples total.

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Project description:Chickpea (Cicer arietinum L.) is an important legume crop, but its yield is greatly affected by the pod borer Helicoverpa armigera. Breeding for durable resistance has been limited by insufficient understanding of host–pest interactions. We conducted the first integrated proteomic analysis of chickpea and its pest, comparing resistant (ICCV506EB), susceptible (ICC3137), and wild relative (IG73016, C. cuneatum) genotypes. Resistant plants triggered rapid defense responses involving jasmonic acid signaling, transcriptional changes, and fatty acid–derived metabolites. In contrast, H. armigera activated detoxification and digestive modulation to counter defenses. Notably, larvae feeding on resistant wild relatives were impaired by linoleic acid derivatives acting as pro-toxins that disrupted growth and immunity. Our study uncovers the molecular basis of chickpea resistance and highlights wild relatives as a reservoir of defense traits. Enhancing early JA-pathway activation through breeding or biotechnology could help develop insect-resistant chickpea varieties, boosting productivity and sustainability.

2025-09-13 | PXD068327 | JPOST Repository

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