Project description:Hypoxia-inducible protein 2 (HIG2), encoded by the gene hypoxia-inducible lipid droplet associated (Hilpda) is an important regulator of intracellular lipid metabolism and immune response. We conducted single cell RNA-seq transcriptome analysis of infarcted brain tissues from wild-type (WT) and conditional Hilpda knockout (cKO) mice at 3 days post-stroke to study the funtion of Hilpda in myeloid cells.
Project description:This program addresses the gene signature associated with brain (cortex) in the tMCAO rat model for stroke. The tMCAO stroke model profiling data was analyzed by identifying genes that were up- and down-regulated at selected p value and fold change in brain cortex of the Sprague Dawley rats following middle cerebral artery occlusion compared to the sham-operated controls.
Project description:Transcriptional profiling of miRNAs from rat brain tissues comparing controls (Sham) with ischemic rats (tMCAO) and neuroprotected rats (RLIP) Internal normalization: ischemic core vs. periischemic and ANOVA comparison across three experimental conditions: Sham, tMCAO and RLIP
Project description:This dataset is a time series (1 hour [h], 4 hours, 24 hours, 48 hours, 1 week [w], and 8 weeks) intended to compare normal functioning left ventricles [lv + lv2] with infarcted [ilv] and non-infarcted left ventricles [nilv]. ilv samples are taken from the region between the LAD artery and the apex on a mouse with myocardial infarction. Lv2 samples are from the same region in a sham operated mouse. Nilv samples are taken from the region above the infartion and the left ventricle [lv] samples mimic that region in a sham mouse. The lv and lv2 samples can be compared as both are from normal functioning hearts. For more information visit http://cardiogenomics.med.harvard.edu/groups/proj1/pages/mi_home.html
Project description:To further development of the role of ZEB1 in microglia, we have employed whole mRNA microarray expression profiling as a discovery platform to identify genes. Mouse microglia from wild type(wt), ZEB1-overexpression(ZEB1-tg) and ZEB1 knock down(ZEB1-kd) mice were isolated before and after tMCAO. The result showed after tMCAO, the wild type, ZEB1-overexpression and ZEB1 knock down microglia displayed a different gene profile, this suggested microglia ZEB1 might play an important role after ischemic stroke.