Project description:Hypoxia-inducible protein 2 (HIG2), encoded by the gene hypoxia-inducible lipid droplet associated (Hilpda) is an important regulator of intracellular lipid metabolism and immune response. We conducted single cell RNA-seq transcriptome analysis of infarcted brain tissues from wild-type (WT) and conditional Hilpda knockout (cKO) mice at 3 days post-stroke to study the funtion of Hilpda in myeloid cells.
Project description:This study employed spatial transcriptomics to profile the expression and spatial distribution of Htr2b, P2ry12, Mrc1, and Ms4a7 in mouse brains 3 days after transient middle cerebral artery occlusion (tMCAO). By capturing genome‑wide transcriptional data with spatial coordinates, we aimed to map the regional expression patterns of these genes across the ischemic core, penumbra, and remote areas, and to delineate their co‑localization relationships with distinct immune and glial cell populations. This spatially resolved approach will provide insights into the topographic organization of serotonergic signaling and neuroimmune interactions during the acute phase of ischemic stroke.
Project description:This program addresses the gene signature associated with brain (cortex) in the tMCAO rat model for stroke. The tMCAO stroke model profiling data was analyzed by identifying genes that were up- and down-regulated at selected p value and fold change in brain cortex of the Sprague Dawley rats following middle cerebral artery occlusion compared to the sham-operated controls.
Project description:To investigate the role of the Jak2/Stat3 pathway in acute cerebral ischemia-reperfusion injury (CIRI), we employed AAV9-mediated gene silencing in a tMCAO rat model. Specifically, AAV9-shNC (control) or AAV9-shJak2 (knockdown) was administered, and analyses were performed at 2 days post-surgery. To further identify downstream targets of Jak2/Stat3 signaling under tMCAO conditions, we conducted DIA proteomics sequencing to compare protein expression profiles between tMCAO+shNC and tMCAO+shJak2 rats.
Project description:Transcriptional profiling of miRNAs from rat brain tissues comparing controls (Sham) with ischemic rats (tMCAO) and neuroprotected rats (RLIP) Internal normalization: ischemic core vs. periischemic and ANOVA comparison across three experimental conditions: Sham, tMCAO and RLIP