Project description:Flatworms can very rapidly attach to and detach from many substrates. In the presented work, we analyzed the adhesive system of the marine proseriate flatworm Minona ileanae. We used light-, scanning-, and transmission electron microscopy to analyze the morphology of the adhesive organs, which are located at the ventral side of the tail plate. We performed transcriptome sequencing and differential RNA-seq for the identification of tail-specific transcripts. Using in situ hybridization expression screening, we identified nine transcripts that were expressed in the cells of the adhesive organs. Knock-down of five of these transcripts by RNA interference led to a reduction of the animal's attachment capacity. Adhesive proteins in footprints were confirmed using mass spectrometry and antibody staining. Additionally, lectin labelling of footprints revealed the presence of several sugar moieties. Furthermore, we determined a genome size of about 560 megabases for M. ileanae. We demonstrated the potential of Oxford Nanopore sequencing of genomic DNA as a cost-effective tool to identify the number of repeats within an adhesive protein and to combine transcripts that were fragments of larger genes. A better understanding of the molecules involved in flatworm bioadhesion paves the way towards developing innovative glues with reversible adhesive properties.
2019-11-12 | PXD013631 | Pride
Project description:Reference transcriptome of Stenostomum brevipharyngium
Project description:The leaf transcriptome of the nickel hyperaccumulator specie Noccaea caerulescens subsp. fimiensis living on serpentine soils were compared to the closely related non-nickel-accumulators Noccaea caerulescens "Viviez" growing on a zinc mining site and Noccaea montana living on serpentine soil, to identity differentially expressed genes potentially involved in Ni hyperaccumulation.
