Equine
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1130668 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Changes in the faecal bacterial microbiota during hospitalization of horses with colic and the effect of different causes of colic
| PRJNA661337 | ENA
Project description:Peritoneal fluid samples were obtained from horses presenting to the Veterinary Teaching Hospital (VTH) with signs of colic. Horses ≥ 2 years of age with no abdominal surgery in the previous 30 days were eligible for inclusion. PF samples were collected by abdominocentesis following standard clinical protocols into sterile blood collection tubes and aliquoted prior to storage at -80°C. Signalment, history, clinical evaluation parameters, diagnosis, treatment, complications, and short-term survival were recorded for each horse treated surgically for colic. After case resolution (discharge or death/euthanasia), horses were assigned to a group (non-strangulating or strangulating disease).
Proteins were extracted by chloroform-methanol precipitation followed by digestion with trypsin. Prior to LC-MS analysis, the peptide amount was normalized according to a BCA assay. Dried peptides were suspended in 5% acetonitrile (ACN) with 0.1% formic acid (FA), and 1μg of peptides from each sample injected into an UltiMate 3000 RSLC nanoflow system coupled to a Q Exactive HF-X mass spectrometer (Thermo Scientific, Wilmington, DE).
Protein identification and quantitation were performed in MaxQuant v2.010 software using the Uniprot Equus caballus, Bos Taurus, Homo sapiens and common lab contaminants databases. Expressed proteins were annotated with known or predicted functions using UniProtKB/Swiss-Prot.
2026-02-11 | PXD060655 | JPOST Repository
Project description:To investigate pre-existing mucosal immunity in equine herpesvirus type 1 (EHV-1) immune and non-immune horses during experimental viral challenge. RNAseq was performed on nasal mucosal swab samples from immune and non-immune horses from pre (d-2), early (d1pi and d3pi), mid (d8pi and d10pi), and late (d18pi) infection.
2024-05-08 | GSE232941 | GEO
Project description:Sixteen severly RAO (Recurrent Airway Obstruction) affected horses were studied. All RAO affected male horses were hybridized with GSM1332974 (Thoroughbred male 1, male reference), and the female horses were with GSM1332975 (Thoroughbred female 2, female reference). Finally results are compared with GSE55266 and two other control horses (SPA-H1-3 and SPA-H1-5) and relatively novel RAO CNVs were reported.
2016-03-07 | GSE63033 | GEO
Project description:Studying the recovery dynamics in different diseases poses many difficulties, as patients often display high heterogeneity in their recovery courses. Moreover, most attempts to study disease dynamics focus on disease progression rather than disease recovery mechanisms. To model recovery dynamics, using severe COVID-19 as the example, we align heterogeneous recovery trajectories via a novel computational scheme applied to longitudinally sampled blood transcriptomes. We thus generate pseudotime trajectories, which we then link to cellular and molecular mechanisms based on cell deconvolution analysis and molecular pathway prediction, thus presenting a unique framework for studying recovery processes over time. Specifically, mature neutrophils displayed a gradual decrease during recovery, allowing superior useability for outcome prediction compared to currently used clinical markers. Further, we discovered a recovery-related regulatory change in gene programs resulting in immune rebalancing between interferon and NFkB activity and the restoration of cell homeostasis. We thus propose regulatory mechanisms governing COVID-19 recovery and suggest mature neutrophils and additional gene markers, as novel clinical biomarkers for disease outcome. Overall, we present a novel clinically relevant computational framework for modeling disease recovery, paving the way for future studies of the recovery dynamics in other diseases and tissues.
| EGAS00001005735 | EGA
Project description:Small RNA isolated from synovial fluid of the metacarpophalangeal joints of horses. Horses either had minimal signs of osteoarthritis based on macroscopic and microscopic joint scoring or early (mild) osteoarthritis. Differential expression of small non-coding RNAs was undertaken.
2020-06-30 | E-MTAB-8409 | biostudies-arrayexpress
Project description:Standardized muscular biopsies of the dorsal compartment of the gluteus medius muscle were performed in 7 horses suffering from equine polysaccharide storage myopathy (PSSM) and 6 sound Norman Cob horses . Gene expression analysis was performed using an equine oligonucleotide microarray which included 384 equine gene probes of the nuclear genome and all the mitochondrial genes.
2009-04-08 | GSE15572 | GEO
Project description:Standardized muscular biopsies of the dorsal compartment of the gluteus medius muscle were performed in 7 horses suffering from equine polysaccharide storage myopathy (PSSM) and 6 sound Norman Cob horses . Gene expression analysis was performed using an equine oligonucleotide microarray which included 384 equine gene probes of the nuclear genome and all the mitochondrial genes. All the samples of PSSM muscles were hybridized against the reference control muscles. This reference was made by pooling together all the mRNA extracted after in vitro transcription from the 6 control muscles of the sound horses. Briefly, the hybridization protocol was adapted from Le Brigand et al. (2006). An open-access long oligonucleotide microarray resource for analysis of the human and mouse transcriptomes. Nucleic Acids Res. 2006 Jul 19;34(12).
2009-04-08 | E-GEOD-15572 | biostudies-arrayexpress
Project description:Equine Papillomavirus Type 2 (EcPV2) appears to be a causal factor for the development of genital especially penile squamous cell carcinomas (SCC) and as such have an important clinical impact on horses. However, the pathomechanisms associated with this cancer transformation are not known, yet. To analyze the host’s and viral transcriptome in EcPV2 affected horses, tissue samples were collected from horses with EcPV2-positive genital lesions as well as from healthy EcPV2-negative horses. Expression levels of host and viral genes were evaluated by RNA-Seq.
2019-12-03 | GSE123533 | GEO
Project description:Differences of the intestinal microbiome between healthy horses and horses with colic
| PRJEB47719 | ENA