Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Comparative transcriptome analysis of engineered Komagataella phaffii

PROVIDER: PRJNA1135688 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29823305_1.fastq.gz	Fastqsanger.gz
	SRR29823305_2.fastq.gz	Fastqsanger.gz
	SRR29823306_1.fastq.gz	Fastqsanger.gz
	SRR29823306_2.fastq.gz	Fastqsanger.gz
	SRR29831175_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Komagataella phaffii

Project description:Komagataella phaffii SNP analysis

| PRJNA592463 | ENA

CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

Project description:The yeast Komagataella phaffii is a promising alternative host for manufacturing of therapeutic proteins. Deletion of unneeded endogenous proteins could increase the secreted titer of recombinant proteins by redirecting cellular resources. Genetic engineering in non-model hosts is hampered by limited annotation of genes, especially essential genes. In this study, we identified the set of endogenous secreted proteins in K. phaffii and attempted to disrupt these genes. We designed, transformed, and sequenced a pooled CRISPR-Cas9 knockout library to determine which genes are essential. With this knowledge, we rapidly disrupted up to 9 consecutive genes in K. phaffii. Engineered strains exhibited a ~20x increase in the production of human serum albumin and a 2x increase in the production of a monoclonal antibody. The pooled CRISPR-Cas9 library and knowledge of gene essentiality reported here will facilitate future efforts to engineer K. phaffii for production of other recombinant therapeutic proteins and enzymes.

2024-08-08 | GSE252605 | GEO

Komagataella phaffii strain:NRRL 64867 | isolate:NRRL 64867

Project description:Komagataella phaffii NRRL 64867 Transcriptome

| PRJNA1360733 | ENA

Komagataella phaffii GS115

Project description:Transcriptome analysis of UV mutagenesis of Komagataella phaffii

| PRJDB19698 | ENA

Project description:RNA-seq analysis of Komagataella phaffii

| PRJNA1006195 | ENA

Effect and mechanism of signal peptide and maltose on recombinant type III collagen production in Pichia pastoris

Project description:In order to investigate the specific mechanism of maltose on the production of recombinant type III collagen by Komagataella phaffii GS115, Two groups were set up, control group C using BMMY medium and experimental group M using BMMY + 1% maltose.

2025-02-01 | GSE225749 | GEO

Identifying improved sites for heterologous gene integration using ATAC-seq

Project description:We analyzed the chromatin accessibility and nucleosome positioning by ATAC-seq of both null and transgene-expressing strains of Komagataella phaffii (Pichia pastoris) under different growth conditions. These data enabled identification of the features that determine performance of various integration sites for transgene expression. Understanding chromatin accessibility and nucleosome positioning can provide further clarity into gene regulation and expression broadly in this organism.

2020-10-02 | GSE154330 | GEO

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

Project description:Prevention of COVID-19 on a global scale will require the continued development of high-volume, low-cost platforms for the manufacturing of vaccines to supply on-going demand. Vaccine candidates based on recombinant protein subunits remain important because they can be manufactured at low costs in existing large-scale production facilities that use microbial hosts like Komagataella phaffii (Pichia pastoris). Here, we report an improved and scalable manufacturing approach for the SARS-CoV-2 spike protein receptor binding domain (RBD); this protein is a key antigen for several reported vaccine candidates. We genetically engineered a manufacturing strain of K. phaffii to obviate the requirement for methanol-induction of the recombinant gene. Methanol-free production improved the secreted titer of the RBD protein by >5x by alleviating protein folding stress. Removal of methanol from the production process enabled scale up to a 1,200 L pre-existing production facility. This engineered strain is now used to produce an RBD-based vaccine antigen that is currently in clinical trials and could be used to produce other variants of RBD as needed for future vaccines.

2021-12-15 | GSE183408 | GEO

Komagataella phaffii

Project description:Komagataella phaffii Transcriptome or Gene expression

| PRJNA890185 | ENA

Project description:Komagataella phaffii GS115 sequencing

| PRJNA1207999 | ENA