Project description:FUDR is a well-studied anti-cancer agent. It is known to inhibit the enzyme thymidylate synthetase, which ultimately interferes with DNA synthesis. In C. elegans, FUDR is used in longevity experiments to induce sterility and avoid transferring worms often. The effect of FUDR on the lifespan of wild-type worms is negligible, however certain genotypes show an extension of lifespan when exposed to FUDR. The mechanisms behind this phenomenon is unclear whether FUDR acts on DNA or RNA. Our data provides the gene expression profile characteristic of wild-type worms exposed to FUDR in comparison to worms grown on regular OP50-seeded plates.
Project description:To identify candidate genes whose expressions were regulated by P. aeruginosa PA01, we used RNA-Seq analysis to study the transcriptomic profiles of mid-L4 worms exposed to P. aeruginosa PA01 for 12 hours. Differential expression analysis was performed by comparing WT worms exposed to P. aeruginosa PA01 for 12 hours versus worms fed E. coli OP50.
Project description:To investigate which genes are up- or down-regulated during L4 sleep in C. elegans mutant aptf-1(gk794) we performed whole genome microarray expression profiling using the C. elegans (V2) Gene Expression Microarray, 4x44K from Agilent Technologies. Approximately 200 sleeping L4 worms were used per sample. As a control we have used N2 C. elegans wild type strain. Mutant condition (aptf-1(gk794)) and control (N2) were done in four replicates.
Project description:To identify genes differentially expressed during the molt, we collected RNA 30-40 minutes after feeding cessation at the start of the fourth larval stage (L4) lethargus. Additional time points for RNA collection were in the mid-L4 stage, approximately four hours prior to lethargus, and in the young adult stage, four hours after lethargus. These samples were interrogated with the Affymetrix C. elegans Genome Array. A total of 1,804 gene transcripts were up regulated, and 1,088 gene transcripts were down regulated, during the L4 lethargus period compared to the L4 and Adult stages (false discovery rate (FDR) < 0.05).