Project description:human somatic cell MRC-5 had different gene expression level compared with that of the adult cells including CCD-1079sk and IMR90. This difference gave the human somatic cell MRC-5 unique characteristics which could then be compared with the human ES cells by genes comparison to show the gene expression difference between human somatic cells and human ES cells.
Project description:Human ES cells had different gene expression level compared with that of the adult cells including CCD-1079sk and IMR90. This difference gave the human ES cells unique characteristics which could then be compared with other species' ES cells or iPS cells like mouse ES cells and Rat iPS cells by the homologue genes comparison to show which species' ES cells or iPS cells were more competitive with the other.
Project description:Intergenic to H19 and Igf2, there is a previously identified centrally conserved domain (ccd ) that includes two sequences with high conservation between human and mouse. At one of these sequence elements there is DNAse1 Hypersentitivity sequence (HSS), CTCF binding and chromatic interaction/looping to sequence upstream of Igf2 on both parental alleles. In earlier studies, removal of ccd sequence from the maternal allele resulted in activation of the normally repressed maternal Igf2 in neonatal limb. We have generated an independent ccd (floxed) deletion allele. Deletion of ccd from both the maternal and paternal allele also results in elevated levels of Igf2 expression from the respective alleles in adult skeletal muscle. Capture C analysis of adult skeletal muscle nuclei uncovers new chromatin interactions in ccd deletion alleles.
Project description:Remaining adult bees in colonies suffering from CCD at apiaries in Florida, California and Pennsylvania were collected during the winter of 2006-2007. The health of CCD colonies was scored at the time of collection as either ï¾severeï¾ or ï¾mildï¾ depending on the apparent strength of the colony. ï¾Historicalï¾ bees, collected prior to the appearance of CCD and hence ostensibly healthy, were collected in 2004 and 2005 from colonies set up on new equipment and not receiving any miticide treatments from apiaries of The Pennsylvania State University near State College, PA. A combination looped, common-reference microarray design was used to compare historical and CCD samples with each other; a blend of RNA isolated from healthy colonies (collected near Urbana, IL in July 2007) served as reference. The microarray experiment compared the guts of bees from mildly and severely afflicted colonies collected in apiaries experiencing CCD on the East Coast (Florida and Pennsylvania) and West Coast (California) with a common reference.
