Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: ultrasonic treatment

PROVIDER: PRJNA1141261 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30017280_1.fastq.gz	Fastqsanger.gz
	SRR30017280_2.fastq.gz	Fastqsanger.gz
	SRR30017281_1.fastq.gz	Fastqsanger.gz
	SRR30017281_2.fastq.gz	Fastqsanger.gz
	SRR30017282_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Evaluation of chromosomal abnormalities and copy number variations in fetuses with ultrasonic soft markers

Project description:This study aimed to evaluate the clinical value of copy number variations (CNVs) in fetuses with ultrasonic soft markers. Among 1131 fetuses, 729 had single ultrasonic soft marker, 322 had two ultrasonic soft markers, and 80 had three or more ultrasonic soft markers. All fetuses underwent single nucleotide polymorphism (SNP) array analysis. Among 1131 fetuses with ultrasonic soft markers, 46 had chromosomal abnormalities. In addition to the 46 fetuses with chromosomal abnormalities consistent with the results of the karyotyping analysis, the SNP array identified additional 6.1% (69/1131) abnormal CNVs. No significant difference was found in the rate of abnormal CNVs among the groups. The SNP array can fully complement conventional karyotyping in fetuses with ultrasonic soft markers, improve detection rate of chromosomal abnormalities, and affect pregnancy outcomes.

2020-12-24 | GSE163799 | GEO

Optimized culturing yields high success rates and preserves molecular heterogeneity, enabling personalized screening for high-grade gliomas

Project description:Robust and reliable and in vitro models are essential in the discovery of new treatment options for high-grade glioma (HGG), however, establishing successful patient-derived cell cultures has posed significant challenges. We established glioma stem-like cell (GSC) cultures from 114 consecutive HGG specimens, obtained via traditional surgical resection and/or ultrasonic aspiration, using fully-dissociated single cells (single cell-derived, SCD) and partially dissociated tissue fragments (3D-derived, 3DD). Copy number profiling assessed genetic similarities, while single-cell RNA sequencing evaluated transcriptomic heterogeneity. Proof-of-concept personalized drug screening was performed using a panel of approved anti-cancer agents. Higher success rates in establishing GSC cultures were obtained from ultrasonic aspirates (SCD and 3DD) and 3DD surgical samples compared to SCD cultures from surgical samples. Combining these approaches in parallel yielded a 96% success rate. In rare cases, copy number variations in original tumor tissue were lost in culture, leading to discernible changes in cell morphology. Single-cell sequencing revealed greater heterogeneity of transcriptomic clusters in ultrasonic aspiration-derived cultures compared to resection-derived cultures. Our study's protocol supported the screening of 20 anti-cancer agents within a clinically-relevant timeframe in 16 of 18 consecutive HGG samples. Our optimized protocol therefore provides a reliable tool for generating HGG cell cultures which capture the tumors’ molecular characteristics and supports precision medicine applications.

2025-05-27 | GSE248469 | GEO

Rapid DNA methylation-based classification of pediatric brain tumours from ultrasonic aspirate specimens

Project description:Cavitating ultrasonic aspirator devices are frequently used in pediatric neurosurgery for efficient microsurgical resection of brain tumours while minimizing tissue damage to surrounding healthy brain. Within this study molecular diagnostics using methylation-based classification of ultrasonic aspirated samples was performed and performance against routine microarray diagnostics was assessed.

2024-02-08 | GSE255044 | GEO

Optimized culturing yields high success rates and preserves molecular heterogeneity, enabling personalized screening for high-grade gliomas [scRNA-seq]

2025-05-27 | GSE248543 | GEO

Metatranscriptomic characterization on anaerobic digestion of Ultrosonic treated Chemical Enhanced Primary Treatment (CEPT) Sludge

Project description:Ultrasonic treatment was used treat the CEPT sludge. Metatranscriptomic analysis was used to characterize the microbial community of anaerobic digestion of Ultrasonic-treated CEPT sludge

| PRJEB20771 | ENA

Evaluation of efficacy and safety of an ultrasonic surgical system and reusable shear for ultrasonic surgical system in colorectal surgery: A prospective, single-center, randomized, open, parallel controlled, non-inferiority trial

Project description:Interventions: Experiment Group A:use the ultrasonic surgical systems (Ezisurg Medical Co., Ltd.) in the colorectal surgery;Experiment Group B:use the reusable shear for ultrasonic surgical system (Ezisurg Medical Co., Ltd.) in the colorectal surgery;Control Group:use the Harmonic Ace+7, 5mm Diameter Shears with Advanced Hemostasis (Ethicon Endo-Surgery,LLC) in the colorectal surgery Primary outcome(s): overall good performance rate Study Design: Parallel

| 36779 | ecrin-mdr-crc

Comparative transcriptome analysis provides insights into the molecular mechanisms of high-frequency hearing differences between the sexes of Odorrana tormota

Project description:Males of the concave-eared frog (Odorrana tormota) have evolved an ultrasonic communication capacity to avoid masking by the widespread background noise of local fast-flowing streams, whereas females exhibit no ultrasonic sensitivity. However, the molecular mechanisms underlying the high-frequency hearing differences between the sexes of O. tormota are still poorly understood. In this study, we sequenced the brain transcriptomes of male and female O. tormota, and compared their differential gene expression. A total of 4,605 differentially expressed genes (DEGs) between the sexes of O. tormota were identified and eleven of them were related to auditory based on the annotation and enrichment analysis. Most of these DEGs in males showed a higher expression trend than females in both quantity and expression quantity. The highly expressed genes in males were relatively concentrated in neurogenesis, signal transduction and ion transport, whereas the up-expressed genes in females were mainly related to energy metabolism, the growth and development regulation of specific auditory cells. This is the first research to reveal the molecular mechanisms of sex differences in ultrasonic hearing between the sexes of O. tormota and will provide new insights into the genetic basis of the auditory adaptation in amphibians during their transition from water to land.

2022-04-20 | GSE188921 | GEO

Ultrasonic surgical aspirate is a reliable source for adult neural progenitor cells (aNPCs)

Project description:Adult neural progenitor cells (aNPCs) are a potential source for cell based therapy for neurodegenerative diseases and traumatic brain injuries. We show that the ultrasonic aspirate samples that are typically considered as a waste after surgery are a great source for aHNPCs.

2017-08-15 | GSE89201 | GEO

Ultrasound-directed emergence of pluripotent-like spheroids from human fibroblasts

Project description:We have developed a gene- and chemical-free method of generating pluripotency from HDF cell, we called pluripotent-like spheroids yielded by ultrasonic stimulus (Physics). Physics were very similar to embryoid bodies (EB) in terms of cluster formation, pluripotency with three germ layer markers, and multi-lineage differentiation potential.

2019-05-30 | GSE82038 | GEO

Project description:Fast start-up and enhancement of CANON process for the low-strength wastewater treatment: tactic and action of ultrasonic

| PRJNA905532 | ENA