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Homo sapiens

ABSTRACT: Gene expression changes upon expression of activated versions of MEK1 and HRAS in MCF10A cells

OTHER RELATED OMICS DATASETS IN: E-GEOD-12764E-GEOD-29327GSE12790

PROVIDER: PRJNA114163 | ENA |

REPOSITORIES: ENA

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Similar Datasets

Gene expression changes upon expression of activated versions of MEK1 and HRAS in MCF10A cells

Project description:MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors Cells were lysed 24 hours post-transfection with collection of total RNA and protein Keywords: Oncogene inducation of gene expression changes

2009-01-12 | GSE12764 | GEO

Transcription profiling by array of human MCF10A cells reveal changes upon expression of activated versions of MEK1 and HRAS

Project description:MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors; Cells were lysed 24 hours post-transfection with collection of total RNA and protein Experiment Overall Design: Overexpression of Oncogenic protein was confirmed via Western blot

2009-01-17 | E-GEOD-12764 | biostudies-arrayexpress

E-GEOD-12764

Project description:Transcription profiling by array of human MCF10A cells over-expressing HRas or MEK1

2025-01-24 | E-GEOD-12764 | ExpressionAtlas

Integrated ChIP-seq and Transcriptomic Profiling of Histone Modifications Across Breast Cancer Progression in MCF10A-Derived Cell Lines [ChIP-Seq]

Project description:Cancer arises from complex epigenetic mechanisms that drive tumorigenesis. Gene regulatory elements play a pivotal role in reprogramming gene expression patterns, facilitating tumor initiation, progression, and metastasis. However, a comprehensive characterization of epigenetic alterations across cancer development remains incomplete, particularly for the spectrum of post-translational histone modifications in the evolution of breast tumors. In this study, we explored the dynamic landscape of epigenetic changes using a breast cancer progression model based on the MCF10A cell line. We integrated ChIP-seq and transcriptomic profiling to investigate the epigenetic heterogeneity across four MCF10A-derived cancer cell lines: normal (MCF10A), HRAS G12V transformed, ductal carcinoma in situ (DCIS), and metastatic (CA1a). Our analysis revealed significant changes in chromatin patterns at regulatory elements, including stage-specific variability in H3K27me3 and H3K9me3-marked heterochromatin domains. These profiles underly the epigenomic changes in each of these cell states to provide insights into human breast cancer progression.

2025-07-05 | GSE271703 | GEO

SNAI1-mediated epithelial-mesenchymal transition confers chemoresistance and cellular plasticity on MCF10A cells by regulating signaling pathways involved in apoptosis and stem cell maintenance

Project description:Stable expression of SNAI1 in MCF10A cells enhanced resistance to cell death and cellular plasticity by regulating signalling pathways involved in apoptotis and stem cell maintenance. To identify changes on gene expression upon SNAI1-induced epithelial-mesenchymal transition, total RNA was purified from MCF10A cells and MCF10A cells stably expressing SNAI1. Three biololgical replicates were used.

2013-07-01 | E-GEOD-42388 | biostudies-arrayexpress

Homo sapiens

Project description:MYC-ER and MYC-ER V394D induced gene expression changes in MCF10A cells

| PRJNA254498 | ENA

Transcriptome analysis of control and RBM6 KO cells before and after DNA damage

Project description:Control and RBM6-KO MCF10A-Hras cells were subjected to RNA-sequencing before and after 12hrs exposure to 5Gy ionizing radiation (IR). Transcriptome analysis revealed a subset of genes that are differentially regulated in RBM6-KO cells compared to control cells. In addition, transcriptome of RBM6-KO cells after IR showed upregulation of DNA damage genes, suggesting impaired DNA repair compared to control cells.

2021-03-23 | GSE167265 | GEO

C/EBPbeta-2 regulation of gene expression in MCF10A cells

Project description:C/EBPbeta-2 results in EMT and ErbB indpendence this project investigated the gene changes in related genes upon C/EBPbeta-2 overexpression in MCF10A cells. We used microarray analysis to detail the global gene expression mediated by C/EBPbeta-2 and identified changes in known EMT genes, however, known ErbB related genes were not altered. MCF10A with or without C/EBPbeta-2 were compared.

2010-03-06 | E-GEOD-15065 | biostudies-arrayexpress

Homo sapiens

Project description:Gene expression changes as a result of E-cadherin loss in an isogenic non-malignant MCF10A and MCF10A CDH1-/- breast cells

| PRJNA255049 | ENA

Gene expression comparison of MCF10A cells with and without extra centrosomes through overexpression of PLK4

Project description:The presence of extra centrosomes is a feature of human tumours. However, it is unclear what are the changes elicited by the presence of these abnormalities. To determine the changes in gene expression induced by centrosome amplification, human mammary epithelial cells, MCF10A, expressing a doxycycline inducible PLK4 cDNA were used. Centrosome amplification was induced for 48 hrs upon the addition of doxycyclin to the culture medium. RNA was purified from MCF10A cells without extra centrosomes (no dox) and with extra centrosomes (dox) and processed for microarray analysis.

2018-02-10 | E-MTAB-6415 | biostudies-arrayexpress

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