Project description:MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors Cells were lysed 24 hours post-transfection with collection of total RNA and protein Keywords: Oncogene inducation of gene expression changes
Project description:MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors; Cells were lysed 24 hours post-transfection with collection of total RNA and protein Experiment Overall Design: Overexpression of Oncogenic protein was confirmed via Western blot