ABSTRACT: Diversity and seasonality of cyanobacteria metabolites in a vertical column of Lake Geneva and impact on the growth rate of model ciliate Tetrahymena pyriformis
Project description:In endolysosomal networks, two hetero-hexameric tethers called HOPS and CORVET are found widely throughout eukaryotes. The ciliate Tetrahymena thermophila is a unicellular organism with elaborate endolysosomal pathways. Curiously, Tetrahymena and related protozoa have lost HOPS. Tetrahymena encodes multiple paralogs of most CORVET subunits, assembled into six distinct hexameric complexes, including a distinct Vps8 subunit. Here we analyze the composition of these complexes immunoisolated by affinity capture using the Vps8-FLAG paralogs as bait.
Project description:Transcriptional profiling of liver tissue from rats fed with standard diet with 1% cholesterol (control diet) and the same diet supplemented with 5% Geneva (test sample1) or 5% Hayward (test sample2) kiwifruit lyophylisate. The goal was to determine the anti-atherogenic effect of kiwi fruit supplementation and their impact on gene expression preventing hepatic steatosis
Project description:We present a comprehensive transcriptome of ciliate T. thermophila using the Illumina RNA-seq platform. The data was generated from the six mRNA samples of growth, starvation and conjugation of Tetrahymena. Despite an AT rich genome, there are about 124.6 million reads mapped to T. thermophila genome. Using these mapped reads, we have significantly improved the previous genome annotation and investigated the gene expression. Besides, our result also provided a comprehensive understanding of the alternative splicing in T. thermophila, and suggested the existence of the regulated unproductive splicing and translation (RUST) in the single-celled eukaryote. RNA-seq for six samples of Tetrahymena growth, starvation and conjugation.
Project description:The interaction between large inland water bodies and the atmosphere impacts the evolution of regional weather and climate, which in turn affects the lake dynamics, thermodynamics, ice-formation, and, therefore, ecosystems. Over the last decades, various approaches have been used to model lake thermodynamics and dynamics in standalone mode or coupled to numerical atmospheric models. We assess a turbulence-closure [Formula: see text] multi-column lake model in standalone mode as a computationally-efficient alternative to a full three-dimensional hydrodynamic model in the case of Lake Geneva. While it struggles to reproduce some short-term features, the multi-column model reasonably reproduces the seasonal mean of the thermal horizontal and vertical structures governing heat and mass exchanges between the lake surface and the lower atmosphere (stratified period, thermocline depth, stability of the water column). As it requires typically two orders of magnitude less computational ressources, it may allow a two-way coupling with a RCM on timescales or spatial resolutions where full 3D lake models are too demanding.
