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An unbiased genomewide screen uncovers 7 genes that drive hematopoietic stem cell fate from mouse embryonic stem cells [scRNA-seq embryoid bodies]

ABSTRACT: An unbiased genomewide screen uncovers 7 genes that drive hematopoietic stem cell fate from mouse embryonic stem cells [scRNA-seq embryoid bodies]

PROVIDER: PRJNA1146392 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30188122.fastq.gz	Fastqsanger.gz
	SRR30188123.fastq.gz	Fastqsanger.gz
	SRR30188124.fastq.gz	Fastqsanger.gz
	SRR30188125.fastq.gz	Fastqsanger.gz
	SRR30188126.fastq.gz	Fastqsanger.gz

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An unbiased genomewide screen uncovers 7 genes that drive hematopoietic stem cell fate from mouse embryonic stem cells [scRNA-seq embryoid bodies]

Project description:Hematopoietic Stem Cells (HSCs) possess the ability to long-term reconstitute all the blood lineages and generate all blood cell types. As such, the in vitro generation of HSCs remains a central goal in regenerative medicine. Despite many efforts and recent advancements in the field, there is still no robust, reproducible and efficient protocol for generating bona-fide HSCs in vitro. This suggests that certain regulatory elements have yet to be uncovered. Here, we present a novel and unbiased approach to identifying endogenous components to specify HSCs from pluripotent stem cells. We performed a genome-wide CRISPR activator screening during mesodermal differentiation from mouse embryonic stem cells (mESCs). Following in vitro differentiation, mesodermal KDR+ precursors were transplanted into primary and secondary immunodeficient NSG mice. This approach led to the identification of seven genes (Spata2, Aass, Dctd, Eif4enif1, Guca1a, Eya2, Net1) that, when activated during mesoderm specification, induce the generation of hematopoietic stem and progenitor cells (HSPCs). These cells are capable of serial engraftment and multilineage output (erythroid, myeloid and T and B lymphoid) in vivo. Single-cell RNA sequencing further revealed that activating these seven genes biases the embryoid bodies towards intraembryonic development, instead of extraembryonic, increasing the number of mesodermal progenitors that can generate HSCs. Our findings underscore the importance of differentiation during the first germ layer specification to generate definitive blood stem cells.

2025-04-23 | GSE274424 | GEO

Expression data from Mst knockout embryoid bodies and wild type embryoid bodies

Project description:Mst1/Mst2 are central components of Hippo pathway. We examined the role of Mst1/Mst2 in ES cell differentiation. In this data set, we include expression data from day 4 and day 8 Mst1/Mst2 knockout ES cell formed embryoid bodies and wild type embryoid body controls.

2014-01-02 | GSE50219 | GEO

Geminin represses mesendoderm cell fate acquisition in embryoid bodies

Project description:Geminin is a small nucleoprotein that neuralizes ectoderm in the Xenopus embryo. Geminin promotes neural fate acquisition of mouse embryonic stem cells: Geminin knockdown during neural fate acquisition decreased expression of neural precursor cell markers (Pax6, Sox1), while increasing expression of Pitx2, Lefty1 and Cited2, genes involved in formation of the mouse node. Here we differentiated mouse embryonic stem cells into embryoid bodies to study Geminin's ability to repress primitive streak mesendoderm fate acquisition. We used microarrays to define the sets of genes that are regulated by Geminin during cell fate acquisition in embryoid bodies, using Dox-inducible Geminin knockdown or overexpression mouse embryonic stem cell lines.

2014-07-01 | GSE39676 | GEO

Genome-wide maps of Rad21 in R1/E embryonic stem cells and R1/E derived Embryoid bodies

Project description:ChIP-seq based determination of Rad21 binding sites in embryonic stem cells (ESC) and ESC derived Embryoid Bodies (EB) identified

2011-05-19 | GSE24029 | GEO

Embryonic stem cells differentiated into embryoid bodies. vs cells grown in regular growth conditions

Project description:Embryonic stem cells differentiated into embryoid bodies. Their expression pattern will be compared to that of the same cells grown in regular growth conditions. A study of the differential gene expression of these two populations should reveal genes whose expression is necessary for the maintenance of the stem cell phenotype. Keywords: other

2008-03-01 | GSE10404 | GEO

Expression data from Mst knockout embryoid bodies and wild type embryoid bodies

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Embryoid Bodies from Mouse R1 ES - 9 day

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UCSF- Bulk RNAseq of differentiation timecourse of embryoid bodies

Project description:Bulk RNAseq of differentiation timecourse of embryoid bodies from KOLF2.2J AAVS1 inducible CRISPRi lines

2025-02-03 | GSE288399 | GEO

Genome-wide maps of Rad21 in R1/E embryonic stem cells and R1/E derived Embryoid bodies

Project description:ChIP-seq based determination of Rad21 binding sites in embryonic stem cells (ESC) and ESC derived Embryoid Bodies (EB) identified Examination of Rad21 binding sites in 2 cell types

2011-05-19 | E-GEOD-24029 | biostudies-arrayexpress

11-Point Time Course Study of Differentiating J1 Embryoid Bodies

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2005-12-06 | GSE3749 | GEO

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