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ABSTRACT:
PROVIDER: PRJNA1147196 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this study, we obtain FoAPY1 transgenic tomato plants (2417-GFP) and control plants (GFP). All target tomato plants were identified by WB using anti-GFP antibody and all transgenic tomato plants has no differences in morphological and growth rate compared with wild type tomato plants. In order to detect the potential functional mechanism of FoAPY1 protein in the host plant, the proteomic analysis was performed to analyze differentially abundant proteins in two type tomato plants
2022-08-09 | PXD035875 | Pride
Project description:The goal of the RNA seq was to investigate the transcriptome changes induced by Pseudmonas syringae pv. tomato J4 in wild-type tomato 'Moneymaker' and transgenic 'Moneymaker' overexpressing the Arabidopsis ELP4 (AtELP4) gene. Results showed that P. syringae pv. tomato J4 induced dramatic transcriptional changes in both the wild-type and transgenic tomato plants. Interestingly, a group of defense genes including PR-5x, Pti5, PR1b1, and CHI3/9/14/17, which are associated with resistance to the hemibiotrophic bacterial pathogen Ralstonia solanacearum, were induced to higher levels in the AtELP4 transgenic tomato than in the wild type at 8 and 24 hr after P. syringae pv. tomato J4 infection. These results indicate that overexpression of AtELP4 in tomato leads to faster and/or stronger induction of some defense genes.
2018-05-01 | GSE97697 | GEO
Project description:The tomato SlWRKY3 transcription factor was overexpressed in cultivated tomato (Solanum lycopersicum)and transgenic plants transcriptome was compared to that of wild-type plants.
2016-03-15 | GSE74504 | GEO
Project description:The tomato SlDREB2 transcription factor was overexpressed in cultivated tomato (Solanum lycopersicum) and transgenic plants tolerance to salinity was compared to that of wild-type plants.
2015-06-01 | GSE64010 | GEO
Project description:To investigate global genome expression changes caused by the ectopic expression of CsMYBF1, we compared the gene expression profiles of the transgenic and wild type tomato fruits by RNA-seq analysis.Fruit samples from at least five plants of each genotype, which were harvested between 7 and 10 days after breaker stage, were pooled for total RNA isolation.A total of 3296 genes were differentially expressed between the transgenic and wild type fruits, and most of them were significantly induced, indicating the widespread influence on the expression of the endogenous tomato genes. Two lines of the tomato transgenic fruits expressing citrus gene CsMYBF1 were compared with two of that from the wild type.
2015-09-22 | E-GEOD-73300 | biostudies-arrayexpress
Project description:To reduce the level of major second messenger inositol-1,4,5-triphosphate (InsP3), we generated transgenic tomato plants (cv. Micro-Tom) that are expressing InsP 5-ptase gene. To understand effects of transgene (InsP 5-ptase) on gene expression we carried out microarray analysis from different parts of transgenic and control (wild type, empty vector control) tomato plants Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in transgenic tomato plants expressing InsP 5-ptase gene Keywords: genetic modification
2008-11-15 | GSE9683 | GEO
Project description:Purpose: Many studies have demonstrated that ZFPs have important functions in many biological processes, including plant growth and development, stress response, and phytohormone response. RanBP2-type zinc finger transcription factors have been characterized in animals and humans. However, their functions remain largely unknown in plants. Therefore, the functions of RanBP ZFPs in plants need further study to shed new insights into the importance of these transcription factors in developmental processes. Methods: The transcriptomes of between overexpression of SlRBZ transgenic and wild-type tomato by RNA-seq analysis were evaluated using the Illumina HiSeq⢠2000 sequencing platform. Raw sequences were filtered and the resulting sets of clean reads were used for the following analysis by Tophat and DEGseq software. qRTâPCR validation was performed using SYBR Green assays. Results: In this study, we identified a RanBP2-type zinc finger protein gene (SlRBZ) in tomato. SlRBZ was constitutively expressed in roots, stems, leaves, flowers and fruits. Overexpression of SlRBZ resulted in etiolated and dwarf phenotypes in tomato. Determination of physiological index showed that chlorophyll, carotenoid, and GAs contents were evidently decreased in transgenic plants. Furthermore, the qRT-PCR and RNA-Seq analyses demonstrated that the transcription of the genes involved in these biosynthesis pathways obviously decreased in SlRBZ-OE plants. In addition, ultrastructural observation by transmission electron microscopy indicated that plastids could not develop into mature chloroplasts with normal chloroplast membrane and thylakoid membrane system in SlRBZ-OE plants. Conclusions: The results suggest that overexpression of SlRBZ may impair the biosynthesis of chlorophyll, carotenoid, and gibberellin through blocking chloroplast development, resulting in etiolation and dwarfism in tomato. The transcriptomes of between overexpression of SlRBZ transgenic and wild-type tomato by RNA-seq analysis were evaluated, in duplicate, using the Illumina sequencing platform.
2016-01-29 | E-GEOD-77340 | biostudies-arrayexpress
Project description:Transcriptome profile analysis of transgenic tomato plants expressing the C4 gene of TYLCV
| PRJNA637749 | ENA
Project description:miR6024 overexpression may lead to changes in the transcriptome profile of tomato plants. Further changes may be noticed on infecting these plants with the necrotrophic pathogen Alternaria solani. These changes can only be gauged by carrying out a comparative transcriptome analysis with the wild type plants under similar conditions. We have used tomato (Pusa Ruby) for generation of miR6024 overexpressing transgenics. Disease study on these plants were carried out with the necrotrophic fungus A. solani. We carried an RNA-seq analysis using Illumina hiseq sequencing of 5 RNA libraries created from leaf tissues of wild type, OVX6024 transgenics and A. solani infected wild type and OVX6024 plants. The analysis revealed that 334 and 781 genes were significantly regulated in the transgenic plants and the infected transgenic plants respectively, with respect to their suitable wild type controls. GO enrichment analysis and pathway analysis have been carried out as well. This work is supported by grants from DBT and SERB, GoI.
2021-07-02 | GSE166013 | GEO
Project description:To reduce the level of major second messenger inositol-1,4,5-triphosphate (InsP3), we generated transgenic tomato plants (cv. Micro-Tom) that are expressing InsP 5-ptase gene. To understand effects of transgene (InsP 5-ptase) on gene expression we carried out microarray analysis from different parts of transgenic and control (wild type, empty vector control) tomato plants; Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in transgenic tomato plants expressing InsP 5-ptase gene Experiment Overall Design: Ripe fruits of wild type, vector control and two transgenic tomato lines expressing InsP 5-ptase gene were selected for RNA extraction and hybridization on Affymetrix microarrays Experiment Overall Design: First two leaves of 10 day old light grown in vitro seedlings of wild type, vector control and two transgenic tomato lines expressing InsP 5-ptase gene were selected for RNA extraction and hybridization on Affymetrix microarrays Experiment Overall Design: 10 day old etiolated root tips of wild type, vector control and two transgenic tomato lines expressing InsP 5-ptase gene were selected for RNA extraction and hybridization on Affymetrix microarrays
2008-11-21 | E-GEOD-9683 | biostudies-arrayexpress