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RNAseq analysis of human neutrophils from people with rheumatoid arthritis

ABSTRACT: RNAseq analysis of human neutrophils from people with rheumatoid arthritis

PROVIDER: PRJNA1147260 | ENA |

REPOSITORIES: ENA

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RNAseq analysis of human neutrophils from people with rheumatoid arthritis

Project description:We report gene expression profiles of blood neutrophils from (1) people newly-diagnosed with rheumatoid arthritis (early RA) before commencement of disease-modifying anti-rheumatic drug therapy, (2) DMARD-refractory RA (severe RA) before commencement of anti-TNF therapy and (3) healthy controls.

2025-05-14 | GSE274598 | GEO

RNAseq analysis of human neutrophils from people with DMARD-refractory rheumatoid arthritis

Project description:RNAseq analysis of human neutrophils from people with DMARD-refractory rheumatoid arthritis

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RNAseq analysis of human neutrophils from people with DMARD-refractory rheumatoid arthritis

Project description:We report gene expression profiles of blood neutrophils from (1) people with DMARD-refractory RA before commencement of anti-TNF therapy and (2) healthy controls.

2025-05-14 | GSE274996 | GEO

RNAseq analysis of peripheral blood and synovial fluid neutrophils from rheumatoid arthritis patients

Project description:We report changes in gene expression that take place in human neutrophils following migration from peripheral blood to synovial joints in patients with rheumatoid arthritis

2020-11-24 | GSE154474 | GEO

Synovial fluid and blood neutrophils from rheumatoid arthritis patients and matched healthy controls

Project description:Synovial fluid and blood neutrophils from 7 rheumatoid arthritis patients and 5 matched healthy controls

2018-07-11 | GSE116899 | GEO

RNAseq analysis of peripheral blood and synovial fluid neutrophils from rheumatoid arthritis patients

Project description:RNAseq analysis of peripheral blood and synovial fluid neutrophils from rheumatoid arthritis patients

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Metabolic profiling of rheumatoid arthritis neutrophils reveals altered energy metabolism that is not affected by JAK inhibition

Project description:Neutrophils play a key role in the pathophysiology of rheumatoid arthritis (RA) where release of ROS and proteases directly causes damage to joints and tissues. Neutrophil function is directly affected by Janus Kinase (JAK) inhibitor drugs, including tofacitinib and baricitinib, which are clinically effective treatments for RA. However clinical trials have reported increased infection rates and transient neutropenia during therapy. The subtle differences in the mode of action, efficacy and safety of JAK inhibitors has been the primary research topic of many clinical trials and systematic reviews, to provide a more precise and targeted treatment to patients. The aim of this study was to determine both the differences in the metabolome of neutrophils from healthy controls and people with RA, and the effect of different JAK inhibitors on the metabolome of healthy and RA neutrophils. Isolated neutrophils from healthy controls (HC) (n=6) and people with RA (n=7) were incubated with baricitinib, tofacitinib or a pan-JAK inhibitor (all 200ng/mL) for 2h. Metabolites were extracted and 1H nuclear magnetic resonance (NMR) was applied to study the metabolic changes. Multivariate analyses and machine learning models show a divergent metabolic pattern in RA neutrophils compared to HC at baseline (F1 score = 86.7%) driven by energy metabolites (ATP, ADP, GTP and glucose). No difference was observed in the neutrophil metabolome when treated with JAK inhibitors. However, JAK inhibitors significantly inhibited ROS production and baricitinib decreased NET production (p-value<0.05). Bacterial killing was not inhibited by JAK inhibitors, indicating their effect on neutrophils is beneficial to inhibit joint damage in RA without impairing host defence. This study highlights altered energy metabolism in RA neutrophils which may explain the cause of their dysregulation in inflammatory disease.

2023-07-11 | MTBLS4766 | MetaboLights

Rheumatoid arthritis

Project description:Rheumatoid arthritis (RA) is a chronic, inflammatory joint disease of unknown etiology and pronounced inter-patient heterogeneity. To characterize RA at the molecular level and to uncover key pathomechanisms, we performed whole-genome gene expression analyses. Synovial tissues from rheumatoid arthritis patients were compared to those from osteoarthritis patients and to normal donors. Keywords: disease state analysis

2004-11-04 | GSE1919 | GEO

Proteomics analysis of Rheumatoid arthritis

Project description:The study aimed to identify proteins associated with rheumatoid arthritis. Dysregulated proteins were linked to inflammation, immune response and oxidative stress.

2025-05-15 | MSV000097902 | MassIVE

Differential DNA methylation in Rheumatoid arthritis

Project description:Genome-wide DNA methylation level was studied to determine whether Rheumatoid arthritis patients (cases) has methylation differences comparing to normal controls in PBLs. We used Illumina HumanMethylation450 BeadChip array to determine the genome-wide DNA methylation difference in PBLs from Rheumatoid arthritis patients (cases) and normal controls Bisulphite converted DNA from the Rheumatoid arthritis patients (cases) and normal controls were hybridized to the Illumina Illumina HumanMethylation450 BeadChip arrays

2013-01-21 | E-GEOD-42861 | biostudies-arrayexpress

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