Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Temporal multiomics gene expression data across human embryonic stem cell derived cardiomyocyte differentiation [Ribo-seq]

ABSTRACT: Temporal multiomics gene expression data across human embryonic stem cell derived cardiomyocyte differentiation [Ribo-seq]

PROVIDER: PRJNA1147306 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Temporal multiomics gene expression data across human embryonic stem cell derived cardiomyocyte differentiation [Ribo-seq]

Project description:Embryonic development has not been fully understood, despite significant advances in molecular and systems-level approaches. Human embryonic stem cells (hESCs) serve as a valuable in vitro model for studying early human developmental processes due to their ability to differentiate into all three germ layers. Here, we present a comprehensive multi-omics dataset generated by differentiating hESCs into cardiomyocytes via the mesodermal lineage, collecting samples at 10 distinct time points. We measured mRNA levels by mRNA sequencing (mRNA-seq), translation levels by ribosome profiling (Ribo-seq), and protein levels by quantitative mass spectrometry. Technical validation confirmed high quality and reproducibility across all datasets, with strong correlations between replicates. This extensive dataset provides critical insights into the complex regulatory mechanisms of cardiomyocyte differentiation and serves as a valuable resource for the research community, aiding in the exploration of mammalian development and gene regulation.

2025-07-11 | GSE274622 | GEO

Temporal multiomics gene expression data across human embryonic stem cell derived cardiomyocyte differentiation

2024-08-14 | MSV000095595 | MassIVE

Temporal multiomics gene expression data across human embryonic stem cell derived cardiomyocyte differentiation [RNA-seq]

Project description:Temporal multiomics gene expression data across human embryonic stem cell derived cardiomyocyte differentiation [RNA-seq]

| PRJNA1147293 | ENA

Temporal multiomics gene expression data across human embryonic stem cell derived cardiomyocyte differentiation [RNA-seq]

2025-07-11 | GSE274620 | GEO

Temporal Quantitative Proteomic Profiling of Murine Embryonic Heart Development

Project description:Comprehensive detail of the molecular mechanisms that govern heart development is essential for identifying the etiology of congenital heart disease. We used high-throughput quantitative proteomics to measure temporal changes in the cardiac proteome at eight critical stages of murine embryonic heart development. We identified more than 7,300 proteins, assessed global temporal changes in protein expression, identified cardiac protein interaction networks, and linked protein dynamics with molecular pathways. Using this dataset, we identified and defined a function for the mevalonate pathway in the regulation of embryonic cardiomyocyte proliferation and cell signaling. Overall, our proteomic datasets are an invaluable resource for studying molecular events that regulate embryonic heart development and that contribute to congenital heart disease.

2023-04-06 | PXD032312 | Pride

RNA-seq of poly(A)-/ribo- or poly(A)+ RNAs from human and mouse ES cells

Project description:We have used RNA-seq to examine circular RNAs from poly(A)-/ribo- RNAs in human and mouse embryonic stem cells, and from from RNase R treated poly(A)-/ribo- RNAs in mouse embryonic stem cells.

2014-08-19 | GSE60467 | GEO

RNA-seq of RNase R treated poly(A)-/ribo- RNAs from H9 cells

Project description:We have used RNA-seq to examine circular RNAs from RNase R treated poly(A)-/ribo- RNAs in human embryonic stem cells Examine circular RNAs in human embryonic stem cells

2013-06-18 | E-GEOD-48003 | biostudies-arrayexpress

A Human Embryonic Stem Cell Reporter Line for Studying Cardiomyocyte Biology

Project description:Human embryonic stem cells (hESCs) can be used to generate scalable numbers of cardiomyocytes for studying cardiac biology, disease modeling, drug screens, and potentially for regenerative therapies. Directed differentiation protocols for cardiomyocytes using hESCs are well established, but methods to isolate highly pure population of cardiomyocytes are limited. Reporter cell lines can be valuable for purification and visualization of cells for such applications. We used CRISPR/Cas9 in hESCs to place an mCherry reporter gene into the MYH6 locus, facilitating a simple method to purify cardiomyocytes. MYH6:mCherry positive cells express atrial and ventricular markers and display a range of cardiomyocyte action potential morphologies. At 20 days of differentiation, MYH6:mCherry+ cells show features characteristic of human cardiomyocytes and can be used successfully to monitor drug-induced cardiotoxicity and oleic acid-induced cardiomyocyte arrhythmia. The MYH6:mCherry hESC reporter line should serve as a useful tool for disease modeling and drug development relevant to cardiomyocyte biology.

2019-01-01 | GSE111365 | GEO

TETs during directed differentiation of human embryonic stem cells to cardiomyocyte

Project description:TETs during directed differentiation of human embryonic stem cells to cardiomyocyte

| PRJNA776283 | ENA

A Human Embryonic Stem Cell Reporter Line for Studying Cardiomyocyte Biology

Project description:A Human Embryonic Stem Cell Reporter Line for Studying Cardiomyocyte Biology

| PRJNA436718 | ENA