Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of SIRT3 as an eraser for H3K9 lactylation by antibody-mediated proximity labeling strategy

ABSTRACT: Identification of SIRT3 as an eraser for H3K9 lactylation by antibody-mediated proximity labeling strategy

PROVIDER: PRJNA1147570 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30219674_1.fastq.gz	Fastqsanger.gz
	SRR30219674_2.fastq.gz	Fastqsanger.gz
	SRR30219675_1.fastq.gz	Fastqsanger.gz
	SRR30219675_2.fastq.gz	Fastqsanger.gz
	SRR30219676_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Identification of SIRT3 as an eraser for H3K9 lactylation by antibody-mediated proximity labeling strategy

Project description:As a novel post-translational modification of histone derived from lactate, lysine lactylation (Kla) links lactate metabolism to epigenetic regulation, playing a role in modulation of gene expression in tumor and immune microenvironment. Evidence so far indicates that HDAC1-3 can catalyze the removal of Kla. However, the regulated targets of HDACs for Kla and functional consequence remain elusive. Herein, we used an antibody-based proximity labeling approach to identify SIRT3 binding to histone H3K9la and catalytic removal of the lactylationoup. The molecular docking results further revealed the mechanism of the binding of Kla peptide to SIRT3. More importantly, SIRT3 can specifically modulate the gene transcription by regulating H3K9la, inhibiting the progression of esophageal cancer cells (ESCC). In conclusion, our work identifies the delactylase of H3K9la and reveal an H3K9la-mediated molecular mechanism catalyzed by SIRT3 for gene transcription regulation in ESCC, and our findings provide an opportunity to investigate the physiological significance of Kla and shed light on the unknown cellular mechanisms controlled by SIRT3.

2024-08-18 | GSE274648 | GEO

SIRT3 functions as an eraser of histone H3K9 lactylation to modulate transcription for inhibiting the progression of esophageal cancer

2025-02-24 | PXD054857 | Pride

Off-the-shelf proximity labeling

Project description:Off-the-shelf proximity labeling

| PRJNA716256 | ENA

BAP-seq proximity labeling of subcellular compartments

Project description:BAP-seq proximity labeling of subcellular compartments

| PRJNA954517 | ENA

SIRT3 functions as an eraser of histone H3K9 lactylation to modulate transcription for inhibiting the progression of esophageal cancer

2025-02-27 | PXD061251 |

RNA proximity labeling in log phase Caulobacter crescentus NA1000

Project description:APEX2 RNA proximity labeling is a powerful method for determining localized RNAs in vivo. APEX2 RNA proximity labeling was adapted to bacterial cells, using an APEX2 fusion to the core scaffold of BR-bodies, RNase E. As a control, APEX2 was also fused to a variant of RNase E that lacks its C-terminal IDR and is unable to form BR-bodies, RNase E delta CTD. RNA proximity labeling was performed, and we observed a similar pattern of enriched RNAs to density centrifugation isolated BR-bodies (Al-Husini et al. Mol Cell 2020).

2025-05-31 | GSE297938 | GEO

Bioluminescence-activated proximity labeling for spatial multi-omics

Project description:Mapping the spatial organization of proteins and cellular interactions is crucial for understanding their biological functions. Herein, we report a biocompatible, multi-functional luminescence-activated proximity labeling (LAP) strategy for profiling subcellular proteomes and cell-cell interactions in live cells and animals. Our method capitalizes on fusing the photocatalyst miniSOG to NanoLuc luciferase, whose bioluminescence activates miniSOG via a resonance energy transfer mechanism, generating reactive oxygen species in situ to mediate proximity labeling. We achieved local transcriptome profiling by combining LAP with next-generation sequencing.

2025-05-10 | GSE276085 | GEO

Bioluminescence-activated proximity labeling for spatial multi-omics

Project description:Bioluminescence-activated proximity labeling for spatial multi-omics

| PRJNA1154663 | ENA

Profiling stress-triggered RNA condensation with photocatalytic proximity labeling

Project description:Profiling stress-triggered RNA condensation with photocatalytic proximity labeling

| PRJNA925537 | ENA

RNA proximity labeling in log phase Caulobacter crescentus NA1000

Project description:RNA proximity labeling in log phase Caulobacter crescentus NA1000

| PRJNA1266958 | ENA