Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MeRIP-seq of Alkbh5/Mettl3 knockout Bone marrow-derived macrophages

ABSTRACT: MeRIP-seq of Alkbh5/Mettl3 knockout Bone marrow-derived macrophages

PROVIDER: PRJNA1148063 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

MeRIP-seq of Alkbh5/Mettl3 knockout Bone marrow-derived macrophages

Project description:Alkbh5/Mettl3loxp/loxp mice were crossed with Lyz2-Cre mice to generate macrophage-specific conditional knockout mice for Alkbh5/Mettl3. Bone marrow-derived macrophages (BMDMs) were isolated from these mice, and MeRIP-seq was performed to investigate the impact of the knockout on RNA N6-Methyladenosine

2025-07-16 | GSE274791 | GEO

MeRIP-seq profiling of m⁶A modifications in Alkbh5-KO and control bone marrow-derived macrophages following M1 polarization

Project description:We performed MeRIP-seq combined with RNA-seq to identify downstream targets of the m⁶A demethylase ALKBH5 in macrophages. Bone marrow-derived macrophages from myeloid-specific Alkbh5 knockout and Alkbh5-fl/fl littermate control mice were polarized to the M1 phenotype with LPS and IFN-γ. m⁶A-enriched RNA was immunoprecipitated using an anti-m⁶A antibody and sequenced on an Illumina NovaSeq 6000 platform. Altered m⁶A peaks in Alkbh5-KO cells were predominantly located within coding sequences, 3′ untranslated regions, and regions near stop codons. Integrative analysis with RNA-seq identified genes with both altered m⁶A methylation and differential expression, revealing the m⁶A epitranscriptomic landscape regulated by ALKBH5 in M1-polarized macrophages.

2026-09-03 | GSE337112 | GEO

MeRIP-seq comparison of Alkbh5 knockout AT2 cells

Project description:We performed RNA sequencing on FACS-purified alveolar type II (AT2) cells isolated from Alkbh5 conditional knockout mice and littermate controls. AT2 cells were collected 14 days after bleomycin-induced lung injury. The MeRIP-seq dataset captures transcriptional changes associated with Alkbh5 deletion in AT2 cells, including the observed upregulation of Amphiregulin (Areg). These data provide a resource for analyzing gene expression programs in regenerating alveolar epithelium.

2026-04-29 | GSE312382 | GEO

MeRIP-seq comparison of Alkbh5 knockout AT2 cells

Project description:MeRIP-seq comparison of Alkbh5 knockout AT2 cells

| PRJNA1373212 | ENA

Identification of ALKBH5 targets in osteosarcoma by methyl RNA immunoprecipitation [MeRIP-seq]

Project description:To identify potential target mRNAs that are demethylated by ALKBH5, meRIP-seq with a m6A antibody was conducted in 143B osteosarcoma cells transfected with scrambled or ALKBH5-siRNA.

2022-06-28 | GSE154529 | GEO

RNA-seq and MeRIP-seq comparison of Mettl3 knockout basal cell derived organoids

Project description:Airway basal stem cells are critical for maintaining epithelial homeostasis and repairing tissue damage, yet the epitranscriptomic mechanisms governing their stemness remain poorly understood. We show that METTL3 deficiency in murine airway basal cells leads to aberrant differentiation, loss of stemness, and impaired organoid formation. RNAseq and MeRIP-seq reveal that METTL3 directly targets the mRNA of Ninj1 for m6A modification, promoting its degradation. Loss of METTL3 leads to Ninj1 upregulation.

2026-07-01 | GSE303982 | GEO

MeRIP-seq to explore the targets of METTL3 in pancreatic cancer

Project description:We performed MeRIP-seq of METTL3-knockdown (KD) MIAPaCa-2 and control cells to detect METTL3-methylated genes and extracted genes whose methylation was lost in METTL3-KD cells compared with control cells. Next, we performed mRNA expression analysis using dates of input samples at MeRIP-seq. Genes whose methylation peaks were abolished by METTL3-KD and whose expression changes were observed were analyzed as candidates for METTL3-regulated genes.

2021-12-13 | GSE190078 | GEO

MeRIP-Seq analysis in thyroid cancer cells knocking down METTL3

Project description:To detect METTL3-mediated m6A methylated transcript in thyroid cancer, we performed MeRIP-Seq of control and METTL3 knockdown BCPAP cells

2025-11-01 | GSE218627 | GEO

ALKBH5 promotes tumor progression by decreasing RIG-I expression mediated by N6-methyladenosine-dependent HNRNPC binding in HNSCC [MeRIP-seq]

Project description:To determine the targets underlying ALKBH5 during head and neck squamouse cell carcinoma progression, Methylated RNA immunoprecipitation (MeRIP) with an m6A specific antibody followed by RNA sequencing (MeRIP-seq) and next generation sequencing were combined to screen the potential targets haboring m6A modificatios and mRNA level alteration after ALKBH5 knockdown in a HNSCC cell line.

2022-04-13 | GSE185885 | GEO

MeRIP-sequencing data from H1975 cells with NC or METTL3-sh

Project description:We use MeRIP-sequencing to evaluate the effect of METTL3 knockdown on the gene changes in H1975 cells.

2020-12-07 | GSE147171 | GEO