Project description:Alkbh5/Mettl3loxp/loxp mice were crossed with Lyz2-Cre mice to generate macrophage-specific conditional knockout mice for Alkbh5/Mettl3. Bone marrow-derived macrophages (BMDMs) were isolated from these mice, and MeRIP-seq was performed to investigate the impact of the knockout on RNA N6-Methyladenosine
Project description:We performed RNA sequencing on FACS-purified alveolar type II (AT2) cells isolated from Alkbh5 conditional knockout mice and littermate controls. AT2 cells were collected 14 days after bleomycin-induced lung injury. The MeRIP-seq dataset captures transcriptional changes associated with Alkbh5 deletion in AT2 cells, including the observed upregulation of Amphiregulin (Areg). These data provide a resource for analyzing gene expression programs in regenerating alveolar epithelium.
Project description:To identify potential target mRNAs that are demethylated by ALKBH5, meRIP-seq with a m6A antibody was conducted in 143B osteosarcoma cells transfected with scrambled or ALKBH5-siRNA.
Project description:Airway basal stem cells are critical for maintaining epithelial homeostasis and repairing tissue damage, yet the epitranscriptomic mechanisms governing their stemness remain poorly understood. We show that METTL3 deficiency in murine airway basal cells leads to aberrant differentiation, loss of stemness, and impaired organoid formation. RNAseq and MeRIP-seq reveal that METTL3 directly targets the mRNA of Ninj1 for m6A modification, promoting its degradation. Loss of METTL3 leads to Ninj1 upregulation.
Project description:We performed MeRIP-seq of METTL3-knockdown (KD) MIAPaCa-2 and control cells to detect METTL3-methylated genes and extracted genes whose methylation was lost in METTL3-KD cells compared with control cells. Next, we performed mRNA expression analysis using dates of input samples at MeRIP-seq. Genes whose methylation peaks were abolished by METTL3-KD and whose expression changes were observed were analyzed as candidates for METTL3-regulated genes.
Project description:To detect METTL3-mediated m6A methylated transcript in thyroid cancer, we performed MeRIP-Seq of control and METTL3 knockdown BCPAP cells
Project description:To determine the targets underlying ALKBH5 during head and neck squamouse cell carcinoma progression, Methylated RNA immunoprecipitation (MeRIP) with an m6A specific antibody followed by RNA sequencing (MeRIP-seq) and next generation sequencing were combined to screen the potential targets haboring m6A modificatios and mRNA level alteration after ALKBH5 knockdown in a HNSCC cell line.
Project description:By performing RNA-seq analysis on bone marrow neutrophils from the Alkbh5-deficient mice and Wild-type littermates undergoing CLP-induced sepsis, we want to investigate the effect of ALKBH5 on transcriptional landscape of mouse bone marrow neutrophils during bacterial infection. Then, we performed gene expression profiling and Gene Ontology enrichment analysis of the significantly differentially expressed genes using data obtained from RNA-seq.