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SP5 and SP8 transcription factors drive primary cilia formation in early embryo

ABSTRACT: SP5 and SP8 transcription factors drive primary cilia formation in early embryo

PROVIDER: PRJNA1148170 | ENA |

REPOSITORIES: ENA

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	SRR30238795.fastq.gz	Fastqsanger.gz

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

Project description:Although specific transcription factors (TFs) are known to regulate cell fate decisions, the degree to which they can stimulate formation of specific cell organelles is less clear. We used a multiomics comparison of the transcriptomes of ciliated and unciliated embryonic cells to identify TFs up-regulated in ciliated cells. We also used conditional genetics in mouse embryos and stem cells and found that the TFs SP5 and SP8 regulate cilia formation and gene expression. In embryos lacking Sp5 and Sp8, primary and motile cilia were shorter than normal and reduced in number across cell types, contributing to situs inversus and hydrocephalus. Moreover, expression of SP8 was sufficient to induce primary cilia in unciliated cells. This work will facilitate the study of cilia assembly using stem cell models and promote further understanding of human ciliopathies.

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [scRNA-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ATAC-seq]

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SP5 and SP8 transcription factors drive primary cilia formation in early embryo [scRNA-seq]

Project description:SP5 and SP8 transcription factors drive primary cilia formation in early embryo [scRNA-seq]

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SP5 and SP8 transcription factors drive primary cilia formation in the early embryo [ATAC-seq]

Project description:SP5 and SP8 transcription factors drive primary cilia formation in the early embryo [ATAC-seq]

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SP5 and SP8 transcription factors drive primary cilia formation in the early embryo [ChIP-seq]

Project description:SP5 and SP8 transcription factors drive primary cilia formation in the early embryo [ChIP-seq]

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Cilia proteome isolated from the multi-ciliated cells of Xenopus laevis embryo epidermis

Project description:We characterized the cilia proteome isolated from the multi-ciliated cells of Xenopus laevis embryo epidermis.

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Sp5 and Sp8 recruit b-catenin to the Tcf1-Lef1 enhanceosome to activate Wnt target gene transcription

Project description:Sp5 and Sp8 recruit b-catenin to the Tcf1-Lef1 enhanceosome to activate Wnt target gene transcription

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Determination of the Sp5 regulated transcriptome in differentiating ES cells

Project description:Sp5 is a target and critical transducer of the Wnt/beta-catenin signaling pathway and is essential for mesoderm differentiation in vertebrate embryos. We established a doxycycline inducible ES cell line to over-express Flag epitope-tagged Sp5 protein in differentiating ES cells which have the capacity to form mesoderm cells in vitro. The goal of this study was to identify the genes/gene networks regulated by Sp5 to understand how it regulates mesoderm differentiation by identifying important target genes which may mediate its activity. Total RNA was extracted from ES cells after 3 days of differentiating following F-Sp5 over-expression from day 2 to 3. We compared the transcriptome of differentially expressed genes between untreated (minus) and doxycycline treated (plus) ES cells to identify Sp5 regulated genes.

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