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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

ABSTRACT: Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

PROVIDER: PRJNA1148170 | ENA |

REPOSITORIES: ENA

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	SRR30238795.fastq.gz	Fastqsanger.gz

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

Project description:Although specific transcription factors (TFs) are known to regulate cell fate decisions, the degree to which they can stimulate formation of specific cell organelles is less clear. We used a multiomics comparison of the transcriptomes of ciliated and unciliated embryonic cells to identify TFs up-regulated in ciliated cells. We also used conditional genetics in mouse embryos and stem cells and found that the TFs SP5 and SP8 regulate cilia formation and gene expression. In embryos lacking Sp5 and Sp8, primary and motile cilia were shorter than normal and reduced in number across cell types, contributing to situs inversus and hydrocephalus. Moreover, expression of SP8 was sufficient to induce primary cilia in unciliated cells. This work will facilitate the study of cilia assembly using stem cell models and promote further understanding of human ciliopathies.

2025-06-25 | GSE274844 | GEO

Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ATAC-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [scRNA-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ATAC-seq]

Project description:Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ATAC-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

Project description:Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [scRNA-seq]

Project description:Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [scRNA-seq]

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SP5/8 transcription factors drive primary cilia formation

Project description:SP5/8 transcription factors drive primary cilia formation

| PRJNA1260973 | ENA

SP5/8 transcription factors drive primary cilia formation

Project description:Sp5/8 are tissue specific zinc finger transcription factors downstream of Wnt signaling pathway. Sp5/8 null embryos display severe posterior axis truncation resembling mutant phenotypes of Wnt/b-catenin signaling pathway. To address the role of Sp5/8 in gastrulation development, we differentiated R1 Wildtype (WT) and Sp5/8 dko Embryonic Stem Cells (ESCs) as gastruloids as described previously (Beccari, L. et al, 2018) to evaluate scRNA transcriptome analysis.

2026-04-22 | GSE296732 | GEO

Bulk-RNA sequencing of E8.25 posterior tissue +2 somites from controls and Sp5/8 double knockout or Wnt3a knockout embryos

Project description:Sp5/8 are Zn finger downstream transcription factors of Wnt/b-catenin signaling pathway. Deletion of Sp5/8 in mice result in severer posterior axis truncation by E9.5 closely resembling Wnt pathway mutants. The goal of this project is to identify differentially expressed genes between controls and Sp5/8 double knockout mutants that could potentially reveal mechanisms of the role of Sp5/8 factors in self-renewal and differentiation of caudal progenitors.

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