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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

ABSTRACT: Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

PROVIDER: PRJNA1148170 | ENA |

REPOSITORIES: ENA

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	SRR30238795.fastq.gz	Fastqsanger.gz

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

Project description:Although specific transcription factors (TFs) are known to regulate cell fate decisions, the degree to which they can stimulate formation of specific cell organelles is less clear. We used a multiomics comparison of the transcriptomes of ciliated and unciliated embryonic cells to identify TFs up-regulated in ciliated cells. We also used conditional genetics in mouse embryos and stem cells and found that the TFs SP5 and SP8 regulate cilia formation and gene expression. In embryos lacking Sp5 and Sp8, primary and motile cilia were shorter than normal and reduced in number across cell types, contributing to situs inversus and hydrocephalus. Moreover, expression of SP8 was sufficient to induce primary cilia in unciliated cells. This work will facilitate the study of cilia assembly using stem cell models and promote further understanding of human ciliopathies.

2025-06-25 | GSE274844 | GEO

Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ATAC-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [scRNA-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [scRNA-seq]

Project description:Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [scRNA-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

Project description:Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ATAC-seq]

Project description:Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ATAC-seq]

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Sp5 and Sp8 recruit b-catenin to the Tcf1-Lef1 enhanceosome to activate Wnt target gene transcription

Project description:Sp5 and Sp8 recruit b-catenin to the Tcf1-Lef1 enhanceosome to activate Wnt target gene transcription

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Determination of the Sp5 regulated transcriptome in differentiating ES cells

Project description:Sp5 is a target and critical transducer of the Wnt/beta-catenin signaling pathway and is essential for mesoderm differentiation in vertebrate embryos. We established a doxycycline inducible ES cell line to over-express Flag epitope-tagged Sp5 protein in differentiating ES cells which have the capacity to form mesoderm cells in vitro. The goal of this study was to identify the genes/gene networks regulated by Sp5 to understand how it regulates mesoderm differentiation by identifying important target genes which may mediate its activity. Total RNA was extracted from ES cells after 3 days of differentiating following F-Sp5 over-expression from day 2 to 3. We compared the transcriptome of differentially expressed genes between untreated (minus) and doxycycline treated (plus) ES cells to identify Sp5 regulated genes.

2016-04-01 | E-GEOD-73082 | biostudies-arrayexpress

Genome-wide binding profiles of Sp5 in differentiation mouse embryonic stem cells

Project description:We have determined the genome-wide binding profile of the transcription factor Sp5. Flag-tagged Sp5 was targeted to the HPRT locus in A2Lox.cre ES cells to allow for Doxycycline inducible expression. ES cells were cultured as embryoid bodies for 2 days to prime them for germ layer differentiation. Cells were then treated with Doxycycline for 24 hrs. We found that Flag-Sp5 prefentially bound to promoters associatd with several growth factor signalling pathways, but most prominently with the Wnt/beta-catenin pathway to selectively promote mesoderm differentiation over neural. These data implicate Sp5 as new regulator of Wnt/beta-catenin target expression to promotes ES cell differentation into the mesoderm lineage. Examination of Sp5 transcription factor binding in differentiating embryonic stem cells.

2016-04-01 | E-GEOD-72989 | biostudies-arrayexpress

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