Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1148752 | ENA |
REPOSITORIES: ENA
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Project description:Salt stress, especially saline-alkali stress, has seriously negative effect on citrus production. Ziyang xiangcheng (Citrus junos Sieb.) (Cj) has been reported as a saline-alkali stress and iron deficiency tolerant citrus rootstock. However, the molecular mechanism of its saline-alkali stress tolerance is still not clear. Two citrus rootstocks and one navel orange scion, Cj, Poncirus trifoliate (Poncirus trifoliata (L.) Raf.) (Pt) and ‘Lane Late’ navel orange (Citrus sinensis (L.) Osb.) (LL), were used in this study. The grafted materials Cj+LL and Pt+LL grown in calcareous soil were used to identify genes and pathways responsive to saline-alkali stress using RNA-seq. The seedlings of Cj and Pt grown in the solutions with different gradient pH value were used to perform a supplement experiment. Comprehensively analyzing the data of RNA-seq, physiology and biochemistry, agronomic traits and mineral elements of Cj+LL, Pt+LL, Cj and Pt, several candidate pathways and genes were identified to be highly regulated under saline-alkali stress. Here, we propose citrate is important for the tolerance to iron deficiency and the jasmonate (JA) biosynthesis and signal transduction pathway may play a crucial role in tolerance to saline-alkali stress in citrus by interacting with other plant hormones, calcium signaling, ROS scavenging system and lignin biosynthesis.
2018-07-06 | GSE98996 | GEO
Project description:Soil salinization-alkalization is a widespread abiotic stress globally, severely impairing crop growth and environmental ecology. Nanomaterials hold significant potential for application in regulating plant stress tolerance due to their unique physical and chemical properties. In this study, the sol-gel method was optimized to synthesize a type of spherical, monodisperse, amorphous mesoporous silica nanoparticles (MSNs) with an average particle size of 40-50 nm and a positive surface charge of 35 mV. A 20 mg/L MSN solution was foliarly applied to two soybean cultivars, Hefeng 50 (HF50) and Henong 95 (HN95), in a pot experiment at the R1 stage to investigate its effects on plant growth and yield-related traits under saline-alkali stress (pH 9.2). MSNs treatment significantly alleviated the growth inhibition induced by saline-alkali stress. Specifically, MSNs significantly increased the plant height, leaf area, root volume, and root surface area of soybean plants under saline-alkali stress. MSNs also increased the dry matter accumulation of plant shoots and roots, improved root activity, and increased the number of pods and seeds per plant. MSNs enhanced cell mechanical strength by activating the synthesis of cuticle and epidermal wax, and maintained intracellular metabolic homeostasis by activating the antioxidant defense system and suppressing the excessive activation of pathways such as the mitogen-activated protein kinase (MAPK) pathway. Additionally, MSNs increased the content of photosynthetic pigments in soybean leaves, thereby enhancing light energy absorption and utilization efficiency, and elevating carbohydrate levels. MSNs promoted the directed translocation of photosynthates to sink organs such as seeds and roots by regulating the activity of carbon metabolism enzymes and source-sink allocation. Taken together, these cumulative effects improved soybean saline-alkali stress tolerance, and ultimately increasing the number of pods per plant, seeds per plant, 100-seed weight, and total yield per plant. The study provides theoretical basis and technical support for the development of nanomaterial-based cultivation practices to improve crop saline-alkali tolerance.
2026-01-20 | GSE316965 | GEO
Project description:Soil salination and alkalization are global problems impairing plant survival by disrupting REDOX homeostasis. Whether melatonin regulates REDOX homeostasis at nitrosative level, and thus affects plant saline-alkali tolerance remains unknown. In saline-alkali stress, excess nitric oxide (NO) causes nitrosative damage in tomato roots. This NO can be degraded by S-nitrosoglutathione reductase (GSNOR), or stimulates caffeic acid O-methyltransferase (COMT) transcript for melatonin synthesis. Melatonin further feedback scavenges excess NO to alleviate nitrosative damage at the whole protein level, indicating by proteome S-nitrosylation. We target plasma membrane H+-ATPase 2 (HA2) and highlight that HA2 is S-nitrosylated at Cys206 in saline-alkali stress, reducing HA activity, H+ efflux, and tolerance by impairing its interaction with 14-3-3 protein 1 (TFT1). In agreement with these observations, COMT-mediated melatonin relieves the HA2 S-nitrosylation to recover its function and saline-alkali tolerance. Therefore, we propose NO and melatonin as a pair of REDOX switches to control HA2 S-nitrosylation and saline-alkali tolerance. Under natural saline-alkali conditions, tomato productivity can be improved by grafting with COMT-, GSNOR-, HA2-overexpression rootstocks or by genetic engineering non-nitrosylated HA2C206S mutants. Using melatonin-NO-HA2 module as a case, this study illuminates a novel molecular function of melatonin and relevant genetic engineering strategies in future agriculture.
2025-04-28 | PXD056815 | Pride
Project description:Soybean is one of the main sources of oil worldwide. Salinity severely affect its yield. GmSIN1 is a NAC transcription factor coding gene. Its overexpression (OE) transgenic lines greatly improved the yield in both common and saline fields. This study focuses on founding changes genes between GmSIN1 OE transgenic seedlings and control seedlings under salt stress or non-salt stress conditions. Illumina Solexa sequencing platform was used for the comparative analysis of transcriptome profiles in the roots and leaves of GmSIN1 OE transgenic seedlings and WEI6823 (control) seedlings under mock or 150 mM NaCl treatment for 6 hrs.
2019-08-01 | GSE93322 | GEO