Project description:Microbial dynamics on different microplastics in the coastal urban water system: the critical roles of extracellular polymeric substances
Project description:Extracellular polymeric substances are degraded by extracellular enzymes purified from seawater communities. Formation of degradation products is measured over time in negative and positive mode with an Agilent 6546 QTOF
2024-11-15 | MSV000096428 | MassIVE
Project description:Extracellular polymeric substances associated to antibiotic resistance genes in microalgae-bacteria systems
Project description:Tracing autotroph and heterotroph photosynthetic catalytic carbon cycling within a microbial mat, confirming biomass 13C incorporation into extracellular polymeric substances through proteomics.
Project description:Extracellular polymeric substances are degraded by extracellular enzymes purified from seawater communities. Formation of degradation products is measured over time in negative and positive mode with an Agilent 6546 QTOF. MS2 Spectral data of degradation products that formed over time
2024-11-18 | MSV000096444 | MassIVE
Project description:16s rRNA sequence for microplastics toxicity to zebrafish
| PRJNA1184701 | ENA
Project description:Prokaryotic community composition and extracellular polymeric substances affect soil microaggregation in semiarid grasslands
Project description:Doxorubicin (DOX) is an effective anthracycline agent used to combat many neoplastic diseases. However, DOX causes cardiovascular toxicity in juvenile and young adult cancer survivors that can lead to future cardiomyopathy. Thus, it is essential to address the cardiovascular toxicity caused by DOX to improve the long-term health of cancer patients. Several studies have suggested that soluble epoxide hydrolase (sEH) and cyclooxygenase-2 (COX-2) are implicated in cardiovascular diseases by impairing vascular health and promoting the transition of Endothelial cells to Mesenchymal cells (EndMT). Given the role of sEH and COX-2 in EndMT cardiovascular toxicity, we aimed to investigate the effect of a dual sEH/COX-2 inhibitor, PTUPB, on DOX-induced EndMT, vascular and cardiac toxicity. We tested the beneficial effect of PTUPB on DOX-induced cardiovascular toxicity in zebrafish.
Project description:LubriShieldTM - a novel permanent coating was invented, and evenly applied to both the internal and external surfaces of indwelling urinary Foley catheters. Without releasing active substances, it effectively prevented pathogens from producing biofilm. The coating was superhydrophilic and incorporated a proprietary anti-fouling ligand, which created a surface that significantly inhibited up to 99% of colonizing uropathogens from forming biofilm for the duration of use without any microbial killing (p< 0.001).RNA-seq analysis revealed that gene expression associated with microbial extracellular polymeric substances formation was significantly downregulated on the coated surfaces. Additionally, microorganisms adhering to LubriShieldTM coated catheters were 78% more susceptible to antibiotics compared to those on uncoated silicone catheters (p=0.004).
Project description:Endocrine disruption (ED) can trigger far-reaching effects on environmental populations, justifying a refusal of market approval for chemicals with ED properties. For the assessment of ED effects on development and reproduction, regulatory decisions mostly rely on apical endpoints from in vivo testing with adult animals. Here, we present a rapid and reproducible data dependent proteomics approach for identifying comprehensive molecular fingerprints interfering with the sexual endocrine system in zebrafish (Danio rerio) embryos as an alternative to animal testing. For this, we have analysed ethinylestradiol as model substances for estrogenic perturbation in a modified zebrafish embryo toxicity test (zFET). These signatures allow for a definition of solid biomarkers as tools in screening approaches and for integration in chronic toxicity studies for identifying suspect ED substances, in the fish early life-stage test (OECD TG 210).
