Project description:Polyphosphate accumulating organisms are responsible for enhanced biological phosphate removal from wastewater, where they grow embedded in a matrix of extracellular polymeric substances. Little is known about the composition and dynamics of those proteins and their production by the different microorganisms. Tomás-Martínez et al., (2022) studied the turnover of proteins and polysaccharides in extracellular polymeric fractions of an enrichment culture of polyphosphate accumulating organisms using an anaerobic-aerobic sequencing batch reactor simulating EBPR conditions. Finally, the carbon source was switched to 13C-labelled acetate to study the protein turnover. Samples were collected at the end of each aerobic phase.
Project description:Extracellular polymeric substances are degraded by extracellular enzymes purified from seawater communities. Formation of degradation products is measured over time in negative and positive mode with an Agilent 6546 QTOF
2024-11-15 | MSV000096428 | MassIVE
Project description:Extracellular polymeric substances associated to antibiotic resistance genes in microalgae-bacteria systems
Project description:Tracing autotroph and heterotroph photosynthetic catalytic carbon cycling within a microbial mat, confirming biomass 13C incorporation into extracellular polymeric substances through proteomics.
Project description:Extracellular polymeric substances are degraded by extracellular enzymes purified from seawater communities. Formation of degradation products is measured over time in negative and positive mode with an Agilent 6546 QTOF. MS2 Spectral data of degradation products that formed over time
2024-11-18 | MSV000096444 | MassIVE
Project description:Prokaryotic community composition and extracellular polymeric substances affect soil microaggregation in semiarid grasslands
Project description:LubriShieldTM - a novel permanent coating was invented, and evenly applied to both the internal and external surfaces of indwelling urinary Foley catheters. Without releasing active substances, it effectively prevented pathogens from producing biofilm. The coating was superhydrophilic and incorporated a proprietary anti-fouling ligand, which created a surface that significantly inhibited up to 99% of colonizing uropathogens from forming biofilm for the duration of use without any microbial killing (p< 0.001).RNA-seq analysis revealed that gene expression associated with microbial extracellular polymeric substances formation was significantly downregulated on the coated surfaces. Additionally, microorganisms adhering to LubriShieldTM coated catheters were 78% more susceptible to antibiotics compared to those on uncoated silicone catheters (p=0.004).
Project description:Characterization and significance of extracellular polymeric substances, reactive oxygen species, and extracellular electron transfer in methanogenic biocathode
| PRJNA736414 | ENA
Project description:Global Insights into Extracellular Polymeric Substances from Activated Sludge Yield, Composition, and Microbial Communities
