Project description:Accumulation of R-loops causes DNA breaks and genome instability, as well as regulates tumorigenesis in a mechanism that remains poorly understood. We knockdown the ribonuclease RNase H1 (RNH1) to generate a cell model of induced R-loop accumulation. We showed that the expression of a large number of genes was disturbed after RNH1 knockdown, including 961 genes with more than twofold differences . The KEGG pathway indicates that the target genes are enriched in the Hippo signaling pathway, Wnt signaling pathway, and other tumor-associated biological processes .
Project description:KYSE510 cells were treated with 100 nM PAF for 24 hours,and then KYSE510 cells were collected for gene expression analysis using Agilent SurePrint G3 Human Gene Expression v3 (8×60K) Microarray. Investigation of the gene network of KYSE510 cells regulated by PAF.
Project description:We report the application of RNA-seq analysis in three kinds of KYSE510 cell lines. One group of cells was transfected with scremble RNA as control and the other two groups of cells were transfected with TCONS_02256997 shRNAs individually to knockdown transcript TCONS_02256997. We did RNA-seq aim to explore the potential target fectors that TCONS_02256997 might effect to and try to explain the mechanism it caused tumor progression.
Project description:Purpose: To fully realize the potential molecular mechanism that LHX2 promotes ESCC progression Methods: Total RNA of LHX2-knockdown KYSE30/KYSE510 and control cells was extracted with TRIzol Reagent. RNA libraries were constructed using an Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol. A total of 150 base paired-end reads were sequenced using the Novaseq 6000 S4 platform. Results: We identified 26008 transcripts in KYSE30 control and KYSE30 LHX2-knockdown cells ,and 25561 transcripts in KYSE510 control and KYSE510 LHX2-knockdown cells. Conclusions: Our study represents the analysis of LHX2-knockdown ESCC cells, generated by RNA-seq.