Project description:Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)
Project description:gnp3_tri33-arabidoseed - preliminary experiment:repetability - WP3 : Biodiversity of seed traits : state of the art - The third experiment is a comparison of the transcriptome of two parents (Bay0,Sha) and two RILs (104, 140) in loop experimental design. Plants are grown in individual pots on a tray.Flowers were tagged on the flowering day using colored tape. Siliques were harvested 10 days after flowering, freezed in liquid nitrogene and stored at -80degreeC until RNA extraction. Keywords: genotype and ecotype comparison
Project description:gnp3_tri33-arabidoseed - preliminary experiment:repetability - WP3 : Biodiversity of seed traits : state of the art - The third experiment is a comparison of the transcriptome of two parents (Bay0,Sha) and two RILs (104, 140) in loop experimental design. Plants are grown in individual pots on a tray.Flowers were tagged on the flowering day using colored tape. Siliques were harvested 10 days after flowering, freezed in liquid nitrogene and stored at -80degreeC until RNA extraction. Keywords: genotype and ecotype comparison 8 dye-swap - CATMA arrays
Project description:Hybrid matings between A. thaliana and A. arenosa result in post zygotic seed lethality resulting in hybridization barrier between the two species. This barrier can be overcome to a large degree by increasing the genome dosage of the maternal genome (i.e. A. thaliana) in a cross between the two species. In this experiment we assayed the transcriptome of an incompatible cross (2x At x 2x Aa) with high seed lethality and a compatible cross (4x At x 2x Aa) using silique tissue at 5 days after pollination. We used microarrays to identify dosage responsive gene in interspecies hybridization Keywords: differential expression
