Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CDK1 regulation of P53 phosphorylation mediates cell cycle arrest and apoptosis of macrophages infected with clinical isolates of Mycobacterium tuberculosis from the Xinjiang region, China

ABSTRACT: CDK1 regulation of P53 phosphorylation mediates cell cycle arrest and apoptosis of macrophages infected with clinical isolates of Mycobacterium tuberculosis from the Xinjiang region, China

PROVIDER: PRJNA1152356 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

CDK1 regulation of P53 phosphorylation mediates cell cycle arrest and apoptosis of macrophages infected with clinical isolates of Mycobacterium tuberculosis from the Xinjiang region, China

Project description:THP-1 Macrophages were infected with Mycobacterium tuberculosis clinical isolates from Xinjiang, China, and H37Rv for 24 hours, respectively, and their transcriptomes were sequenced to investigate the specific biological processes that occur after infection of macrophages with Mycobacterium tuberculosis clinical isolates from Xinjiang, China.

2025-08-23 | GSE275640 | GEO

PP2A inhibitors arrest G2/M transition through JNK/Sp1-dependent down-regulation of CDK1 and autophagy-dependent up-regulation of p21

Project description:Protein phosphatase 2A (PP2A) plays an important role in the control of the cell cycle. We previously reported that the PP2A inhibitors, cantharidin and okadaic acid (OA), efficiently repressed the growth of cancer cells. In the present study, we found that PP2A inhibitors arrested the cell cycle at the G2 phase through a mechanism that was dependent on the JNK pathway. Microarrays further showed that PP2A inhibitors induced expression changes in multiple genes that participate in cell cycle transition. To verify whether these expression changes were executed in a PP2A-dependent manner, we targeted the PP2A catalytic subunit (PP2Ac) using siRNA and evaluated gene expression with a microarray. After the cross comparison of these microarray data, we identified that CDK1 was potentially the same target when treated with either PP2A inhibitors or PP2Ac siRNA. In addition, we found that the down-regulation of CDK1 occurred in a JNK-dependent manner. Luciferase reporter gene assays demonstrated that repression of the transcription of CDK1 was executed through the JNK-dependent activation of the Sp1 transcription factor. By constructing deletion mutants of the CDK1 promoter and by using ChIP assays, we identified an element in the CDK1 promoter that responded to the JNK/Sp1 pathway after stimulation with PP2A inhibitors. Cantharidin and OA also up-regulated the expression of p21, an inhibitor of CDK1, via autophagy rather than PP2A/JNK pathway. Thus, this present study found that the PP2A/JNK/Sp1/CDK1 pathway and the autophagy/p21 pathway participated in G2/M cell cycle arrest triggered by PP2A inhibitors. Quality-checked RNAs were then transcribed with the First-Strand cDNA Synthesis Kit(Agilent)and their expression data obtained using Agilent 4×44 K Human Whole-Genome 60-mer oligonucleotide microarrays according to the protocols by the manufacturer.

2018-12-06 | GSE114288 | GEO

Xinjiang fish

Project description:Xinjiang fish

| PRJNA1188641 | ENA

human oral metagenome isolate:China: Xinjiang

Project description:human oral metagenome isolate:China: Xinjiang Raw sequence reads

| PRJNA1226964 | ENA

human oral metagenome isolate:China: Xinjiang

Project description:human oral metagenome isolate:China: Xinjiang Raw sequence reads

| PRJNA1227399 | ENA

human oral metagenome isolate:China: Xinjiang

Project description:human oral metagenome isolate:China: Xinjiang Raw sequence reads

| PRJNA1227298 | ENA

The microeukaryotic community in lake ecosystems of the Xinjiang region, China

Project description:The microeukaryotic community in lake ecosystems of the Xinjiang region, China Raw sequence reads

| PRJNA825691 | ENA

Chlorella vulgaris isolate:Isolated from Xinjiang, China

Project description:Chlorella vulgaris isolate:Isolated from Xinjiang, China Genome sequencing

| PRJNA730353 | ENA

Halomonas sp. PA5 isolate:Xinjiang provance, China

Project description:Halomonas sp. PA5 isolate:Xinjiang provance, China Genome sequencing

| PRJNA627649 | ENA

Differential expression of lncRNA in peripheral blood lymphocytes of Xinjiang Kazakh patients with essential hypertension

Project description:Investigate long non-coding RNA (lncRNA) expression characteristics in the peripheral blood lymphocytes of Xinjiang Kazakh people with essential hypertension.

2023-06-10 | GSE234085 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data