Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1154319 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Mesembryanthemum Crystallinum, a facultative CAM plant, shifts from C 3 to CAM photosynthesis under
salt stress, enhancing water use efficiency due to inverse stomatal patterns. Exploring the mechanisms
of this transition could improve salt tolerance in C 3 crops. We used transcriptomics, proteomics, and
targeted metabolomics every 8 hours to track molecular shifts during this transition.
2023-10-07 | MSV000093065 | MassIVE
Project description:Mesembryanthemum Crystallinum, a facultative CAM plant, shifts from C 3 to CAM photosynthesis under
salt stress, enhancing water use efficiency due to inverse stomatal patterns. Exploring the mechanisms
of this transition could improve salt tolerance in C 3 crops. We used transcriptomics, proteomics, and
targeted metabolomics every 8 hours to track molecular shifts during this transition.
2023-10-07 | MSV000093065 | GNPS
Project description:High light stress in subtropical and tropical regions strongly limits agricultural production due to photo-oxidative damage, decreased growth and yield. Here, we investigated whether beneficial microbes can protect plants under high light stress. We show that Enterobacter sp. SA187 (SA187) assists Arabidopsis in maintaining growth under high light stress, reducing the accumulation of reactive oxygen species (ROS) and maintaining photosynthesis. Under high light stress, SA187 induces dynamic transcriptional changes related to a fortified iron metabolism and redox system in Arabidopsis. A genetic analysis shows that SA187-induced plant high light stress tolerance is mediated by ethylene signaling via the transcription factor EIN3 to enhance iron metabolism. In summary, we show that Arabidopsis interaction with SA187 results in sustained photosynthesis under high light stress suggesting that beneficial microbes could be effective and cheap means for enhancing high light stress tolerance in crops.
2024-12-05 | GSE251796 | GEO
Project description:In eubacteria, replacement of one σ factor in the RNA polymerase (RNAP) holoenzyme by another one changes the transcription pattern. Cyanobacteria are eubacteria characterized by oxygenic photosynthesis and they typically encode numerous group 2 σ factors that closely resemble the essential primary σ factor. A mutant strain of the model cyanobacterium Synechocystis sp. PCC 6803 without functional group 2 σ factors (named as ΔsigBCDE) could not acclimate to heat, high salt, or bright light stress but in standard conditions ΔsigBCDE grew only 9% slower than the control strain. One-fifth of the genes in ΔsigBCDE were differently expressed compared to the control strain in standard growth conditions and several physiological changes in photosynthesis, and pigment and lipid compositions were detected. To directly analyze the σ factor content of RNAP holoenzyme in vivo, a His-tag was added to the γ subunit of RNAP in Synechocystis and RNAPs were collected. The results revealed that all group 2 σ factors were recruited by RNAP in standard conditions, but recruitment of SigB and SigC increased in heat stress, SigD in bright light, SigE in darkness and SigB, SigC and SigE in high salt, explaining the poor acclimation of ΔsigBCDE to these stress conditions. Cells from cyanobacteria Synechocystis sp. PCC 6803 named as control strain (CS) and a mutant strain without any functional group 2 sigma factors, ΔsigBCDE, were harvested (A730=1, 40 mL) directly from standard growth conditions (continuous illumination at the PPFD of 40 µmol m-2s-1, 32°C, ambient CO2). From three to four independent experiments were performed at each conditions.
2015-09-15 | E-GEOD-69981 | biostudies-arrayexpress
Project description:Mesembryanthemum crystallinum, a facultative CAM plant, shifts from C3 to CAM photosynthesis under salt stress, enhancing water use efficiency due to inverse stomatal patterns. Exploring the mechanisms of this transition could improve salt tolerance in C3 crops. We used transcriptomics, proteomics, and targeted metabolomics every 8 hours to track molecular shifts during this transition. Results confirmed changes in CAM photosynthesis, starch biosynthesis, degradation, and glycolysis/gluconeogenesis. Transcripts displayed greater circadian regulation than proteins. Oxidative phosphorylation was crucial, with the inositol pathway, involving methylation and phosphorylation, potentially initiating the transition. V-type ATPases showed consistent transcription regulation, aiding in vacuolar osmotic pressure maintenance. ABI1, a major component in the ABA signaling pathway, could be the trigger for the salt-induced transition, as it inhibits ABA-dependent stomatal closure. Our work highlights the pivotal role of ABA pathways in the C3 to CAM shift.
2023-10-13 | MSV000093105 | MassIVE
Project description:Salt stress is one of the abiotic stresses that adversely affect plant growth and agricultural productivity all over the word. Root is the organ that immediately suffers salt stress in soil, and thus the ability of roots to adapt to high salinity is critical for salt stress tolerance in plants. During a long-term evolution, plants have developed a variety of strategies to respond to salt stress. The mechanisms of salt stress response are complicated and are stilly largely unknown. In this study, through the screening of Arabidopsis mutants that are sensitive to salt stress, we identified a mutant itpk4, that displayed reduced root growth, reduced seeds germination, and increased root hairs under salt stress compared with wild type plants. in future, the molecular mechanism underlying the role of ITPK4 in root elongation under high.
2024-12-31 | GSE252395 | GEO
Project description:High light stress in subtropical and tropical regions strongly limits agricultural production due to photo-oxidative damage, decreased growth and yield. Here, we investigated whether beneficial microbes can protect plants under high light stress. We show that Enterobacter sp. SA187 (SA187) assists Arabidopsis in maintaining growth under high light stress, reducing the accumulation of reactive oxygen species (ROS) and maintaining photosynthesis. Under high light stress, SA187 induces dynamic transcriptional changes related to a fortified iron metabolism and redox system in Arabidopsis. A genetic analysis shows that SA187-induced plant high light stress tolerance is mediated by ethylene signaling via the transcription factor EIN3 to enhance iron metabolism. In summary, we show that Arabidopsis interaction with SA187 results in sustained photosynthesis under high light stress suggesting that beneficial microbes could be an effective and inexpensive means for enhancing high light stress tolerance in crops.
2025-05-06 | PXD047231 | Pride
Project description:In eubacteria, replacement of one σ factor in the RNA polymerase (RNAP) holoenzyme by another one changes the transcription pattern. Cyanobacteria are eubacteria characterized by oxygenic photosynthesis and they typically encode numerous group 2 σ factors that closely resemble the essential primary σ factor. A mutant strain of the model cyanobacterium Synechocystis sp. PCC 6803 without functional group 2 σ factors (named as ΔsigBCDE) could not acclimate to heat, high salt, or bright light stress but in standard conditions ΔsigBCDE grew only 9% slower than the control strain. One-fifth of the genes in ΔsigBCDE were differently expressed compared to the control strain in standard growth conditions and several physiological changes in photosynthesis, and pigment and lipid compositions were detected. To directly analyze the σ factor content of RNAP holoenzyme in vivo, a His-tag was added to the γ subunit of RNAP in Synechocystis and RNAPs were collected. The results revealed that all group 2 σ factors were recruited by RNAP in standard conditions, but recruitment of SigB and SigC increased in heat stress, SigD in bright light, SigE in darkness and SigB, SigC and SigE in high salt, explaining the poor acclimation of ΔsigBCDE to these stress conditions.
2015-09-15 | GSE69981 | GEO
Project description:Salinity stress in wheat affects physiological and biochemical parameters in tissues that alter plant development and ultimately lower crop yield. Shoot tissues are the most sensitive to salinity in wheat plants and accumulate salt over time through the transpiration stream. Rising NaCl concentrations impose physiological responses in leaf tips rather than in leaf bases and align salt effects with the basipetal developmental gradient of the monocot leaf. The role of metabolic processes in generating and responding to this phenotype can be explored by linking distinct changes in ion distributions to those of enzymes from the base to the tip of leaves under salt stress. We confirmed that enzymes for methionine synthesis and lipid degradation pathways increase, concomitantly with proteins in jasmonate synthesis which are key players in plant salt stress-induced responses. Combining the use of Differential Abundance of Protein analysis and Weighted Correlation Network Analysis we have focused on identifying key protein hubs associated with negative salt responses, shedding light on potential sites of salt sensitivity as targets for enhancing salt tolerance in wheat. We found chloroplast protein synthesis machinery, including the 30S and 50S ribosomal proteins, DNA-directed RNA polymerases, and protein synthesis elongation factors, were significantly reduced in abundance and correlated with the altered K+/Na+ ratio along salt-stressed wheat leaves. Additionally, the ATP-dependent caseinolytic protease and filamentous temperature-sensitive H protease, involved in chloroplast protein homeostasis, show decreased abundance with salt. The complex interplay of these processes in and across the leaf affects overall plant viability under salt stress.
2025-05-07 | PXD059765 | Pride
Project description:Here we report that FERONIA (FER) integrates phyB-mediated light signaling pathway to control salt stress response. Mutation in phytochrome B (phyB) largely suppresses the dwarfism and leaf bleaching phenotype of fer-4 mutant under salt stress. FER interacts with and phosphorylates the N-terminal domain of phyB. Mutation in fer-4 or disruption of the FER-mediated phosphorylation sites of phyB at Ser106 and Ser227 leads to a retention of phyB in the photobodies under dark conditions, suggesting that FER-mediated phosphorylation accelerates the dark reversion of phyB. Interestingly, salt stress slows down the dark reversion of phyB via the inhibition of the kinase activity of FER, and mutation of phyB or overexpression of PIF5 attenuates the salt-induced growth inhibition. Together, our study indicates that the plasma membrane-localized FER determines the dark reversion rate of phyB in the nucleus via a signature of phosphorylation and thus coordinates the extracellular stress signals and nuclear outputs to dynamically control plant growth and survival under stress conditions.
2021-11-08 | GSE188335 | GEO