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The influence of microRNA-455-3p on gene expression in the developing chick limb bud

ABSTRACT: The influence of microRNA-455-3p on gene expression in the developing chick limb bud

PROVIDER: PRJNA1157752 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30568778_1.fastq.gz	Fastqsanger.gz
	SRR30568778_2.fastq.gz	Fastqsanger.gz
	SRR30568779_1.fastq.gz	Fastqsanger.gz
	SRR30568779_2.fastq.gz	Fastqsanger.gz
	SRR30568780_1.fastq.gz	Fastqsanger.gz

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The influence of microRNA-455-3p on gene expression in the developing chick limb bud

Project description:MicroRNA-455 is a key microRNA in cartilage, strongly induced by Sox9. Overexpression of miR-455-3p in the developing chick limb bud at HH18-19 causes a reduction in limb bud size at HH24-25.

2025-02-26 | GSE276509 | GEO

Long non-coding RNA HOTTIP promotes CCL3 expression and induces cartilage degradation by sponging miR-455-3p

Project description:Long non-coding RNAs (lncRNAs) play pivotal roles in diseases such as osteoarthritis (OA). However, knowledge of the biological roles of lncRNAs is limited in OA. We aimed to explore the biological function and molecular mechanism of HOTTIP in chondrogenesis and cartilage degradation. We used the human mesenchymal stem cell (MSC) model of chondrogenesis, in parallel with, tissue biopsies from normal and OA cartilage to detect HOTTIP, CCL3, and miR-455-3p expression in vitro. Biological interactions between HOTTIP and miR-455-3p were determined by RNA silencing and overexpression in vitro. We evaluated the effect of HOTTIP on chondrogenesis and degeneration, and its regulation of miR-455-3p via competing endogenous RNA (ceRNA). Our in vitro ceRNA findings were further confirmed within animal models in vivo. Mechanisms of ceRNAs were determined by bioinformatic analysis, a luciferase reporter system, RNA pull-down, and RNA immunoprecipitation (RIP) assays. We found reduced miR-455-3p expression and significantly upregulated lncRNA HOTTIP and CCL3 expression in OA cartilage tissues and chondrocytes. The expression of HOTTIP and CCL3 was increased in chondrocytes treated with interleukin-1β (IL-1β) in vitro. Knockdown of HOTTIP promoted cartilage-specific gene expression and suppressed CCL3. Conversely, HOTTIP overexpression reduced cartilage-specific genes and increased CCL3. Notably, HOTTIP negatively regulated miR-455-3p and increased CCL3 levels in human primary chondrocytes. Mechanistic investigations indicated that HOTTIP functioned as ceRNA for miR-455-3p enhanced CCL3 expression. Taken together, the ceRNA regulatory network of HOTTIP/miR-455-3p/CCL3 plays a critical role in OA pathogenesis and suggests HOTTIP is a potential target in OA therapy.

2019-08-12 | PXD014988 | Pride

microRNA-Seq of Gallus gallus embryo tissues: Undetermined Presomitic Mesoderm (PSM), Determined PSM, and Limb bud

Project description:This analysis aims at finding the microRNAs present in three chick embryo undifferentiated tissues (pools of tissues): Undetermined Presomitic Mesoderm (PSM-U), Determined Presomitic Mesoderm (PSM-D) and Distal limb bud (Limb).

2021-10-25 | E-MTAB-8176 | biostudies-arrayexpress

Effect of modulation of microRNA-455-3p on gene expression in SW1353 human chondrosarcoma cells

Project description:MicroRNA-455 is a key microRNA in cartilage, strongly induced by Sox9. In order to understand its function, we have overexpressed (miR-455-3p mimic) or inhibited (miR-455-3p inhibitor) miR-455 compared to appropriate controls and measured gene expression.

2025-02-26 | GSE276347 | GEO

RNA-Seq analysis miR-455-3p overexpression effect on breast cancer cell line

Project description:MiR-455-3p has been reported to suppress the expression of ROCK2, HOXB5, hTERT, and RUNX2, which are involved in multiple biological processes including renal fibrosis, chondrogenic development and differentiation, Alzheimer’s disease, preeclampsia, and tumorigenesis. Here, we showed that miR-455-3p broadly inhibited TGF-beta-induced EMT by repressing the expression of Smad2, ZEB1, and HDAC2 in breast cancer cells, and that miR-455-3p loss-of-function promotes the acquisition of cell invasive properties and the ability to metastasize. These results indicate that miR-455-3p represents an important node that inhibits metastasis of breast cancer.

2019-09-06 | GSE129146 | GEO

Roles of Mettl14 in mouse limb bud development

Project description:m6A regulates virtually every step in RNA metabolism. However, its toles in limb development remains largely unknown. To understand the roles, we created a limb bud-specific conditional knockout (cKO) mice and control heterozygous (cHet) mice of the Mettl14 gene, which encodes an essential subunit in the m6A methyltransferase complex METTL3/METTL14. We harvested limb buds from the mice on E12.5 and applied the proteins to quantitative mass spectrometry to understand how the depletion of Mettl14 affected the proteomes.

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Project description:During limb development, fibroblast growth factors (FGFs) govern proximal-distal outgrowth and patterning. FGFs also synchronize developmental patterning between the proximal-distal and anterior-posterior axes by maintaining sonic hedgehog (SHH) expression in cells of the zone of polarizing activity (ZPA) in the distal posterior mesoderm. SHH, in turn, maintains FGFs in the apical ectodermal ridge (AER) which caps the distal tip of the limb bud. Crosstalk between FGF and SHH signaling is critical for patterned limb development, but the mechanisms underlying this feedback loop are not well characterized. Implantation of FGF beads in the proximal posterior limb bud can maintain SHH expression in the former ZPA domain (evident 3hrs after application), while prolonged exposure (24hrs) can induce SHH outside of this domain. Although temporally and spatially disparate, comparative analysis of transcriptome data from these different populations enriched molecules required for FGF-mediated SHH regulation.

2018-12-18 | GSE114661 | GEO

Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray)

Project description:Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray)

| PRJNA472076 | ENA

Genome wide analysis of chick limb bud tissue treated with FGF2 for 3 hrs

Project description:Genome wide analysis of chick limb bud tissue treated with FGF2 for 3 hrs

| PRJNA472073 | ENA

Bhlha9 deficiency effect in limb bud

Project description:Analysis of mouse limb bud (E10.5) lacking the Bhlha9 gene. Bhlha9 knockout mouse shows syndactyly and poliosis in the limb. This microarray results provides insight into the molecular mechanisms underlying Bhlha9 function in the limb development DNA microarray analysis was performed using Affymetrix mouse genome 430 2.0 array. RNA samples were obtained from the whole limb bud of the E10.5 wild-type and Bhlha9 knockout embryos described above. Total RNA (200 ng) was reverse-transcribed and biotinylated using the GeneChip 3â² IVT Express Kit (Affymetrix). The microarray data were summarized using the MAS 5.0 method.

2016-05-31 | E-GEOD-81223 | biostudies-arrayexpress

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