Project description:Transcriptional profiling of Mycobacterium smegmatis comparing the effects of wild type levels of CarD expression with CarD depletion. Transcriptional profile of the mgm1701 control strain was compared with that of mgm1703, a strain where CarD transcription is regulated by a Tet-On system. Both strains were grown in the absence of anhydrotetracline for 13 hours in nutrient rich broth to allow for depletion of CarD transcription in mgm1703. Two condition experiment, control (mgm1701) vs CarD depletion (mgm1703). 3 biological replicates of each strain.
Project description:Transcriptional profiling of Mycobacterium smegmatis comparing the effects of wild type levels of CarD expression with CarD depletion. Transcriptional profile of the mgm1701 control strain was compared with that of mgm1703, a strain where CarD transcription is regulated by a Tet-On system. Both strains were grown in the absence of anhydrotetracline for 13 hours in nutrient rich broth to allow for depletion of CarD transcription in mgm1703.
Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.
Project description:CarD is essential transcription factors in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strains with CarD-FLAG (LK2539), where an additional copy of CarD under anhydrotetracycline inducible promoter was stably inserted into the genome. CarD-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.
Project description:CsrL (MSMEG_5890) associates with CarD and RNA polymerase in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strain with CrsL (LK3051), where an additional copy of CrsL under anhydrotetracycline inducible promoter was stably inserted into M. smegmatis genome. CrsL-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.
Project description:CarD is an essential transcription factor for Mycobacterium smegmatis. We identified a new interaction partner of CarD and named it CrsL (MSMEG_5890). To address the genome-wide effect of CrsL, we generated knockdown strains for both carD (MSMEG_6077) and crsL (MSMEG_5890) genes in Mycobacterium smegmatis. We aimed to compare the differentially expressed genes of crsL knockdown and correlate them with the ones of carD knockdown to describe the role of CrsL in transcription.
