Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CUT&Tag in K562, HCT116, RPE1 and Jurkat cells

ABSTRACT: CUT&Tag in K562, HCT116, RPE1 and Jurkat cells

PROVIDER: PRJNA1159305 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR30621818_1.fastq.gz	Fastqsanger.gz
	SRR30621818_2.fastq.gz	Fastqsanger.gz
	SRR30621819_1.fastq.gz	Fastqsanger.gz
	SRR30621819_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 153

Similar Datasets

Aalysis of replication timing in the RPE1 and HCT116 cell line

Project description:Mapping replication timing in the RPE1 and HCT116 cell lines revealed differences in replication dynamics and replication domain structures in the two cell lines

2018-07-01 | GSE110295 | GEO

CUT&Tag in K562, HCT116, RPE1 and Jurkat cells

Project description:The essential role of the core histones (H2A, H2B, H3 and H4) in DNA packaging has been well understood for decades, but the function of the linker histone (H1) remains enigmatic. H1 compacts nucleosome arrays in vitro, but the multiple H1 subtypes and variants encoded in mammalian genomes (11 in both mice and humans) hamper genetic studies to determine H1 function in vivo. Challenging the prevailing view that linker histones are a general feature of heterochromatin, here we show a critical requirement for linker histones in the function of Polycomb Repressive complex 2 (PRC2). Through a CRISPR/Cas9 genetic screen in a fluorescent reporter cell line responsive to PRC2 perturbation, we identified an essential requirement for the poorly characterised gene CRAMP1 in PRC2-mediated repression. CRAMP1 localises to the promoters of all expressed H1 genes where it acts as a positive transcriptional regulator. Ablation of CRAMP1 provides a unique tool to simultaneously deplete all linker histones, which results in selective decompaction of H3K27me3-marked loci and derepression of PRC2 target genes without concomitant loss of PRC2 occupancy or enzymatic activity. Strikingly, in contrast to the broad genomic distribution previously ascribed to H1, we find that linker histones selectively localise to genomic loci marked by H3K27me3 across diverse cell types and organisms. Altogether, our data demonstrate a prominent role for linker histones in epigenetic repression by PRC2.

2025-06-13 | GSE276857 | GEO

ChIP-seq of old and new H3.3 in RPE1-hTERT cells

Project description:On of the H3.3 genes (H3F3B) was C-terminally tagged with a recombination-induced tag exchange (RITE) tag in human RPE1-hTERT cells to follow old (V5 tag) and new (FLAG tag) H3.3 and measure the dynamics of H3.3. New H3.3 was induced by activating Cre recombinase with 4-hydrotamoxifen (4OHT). ChIP-seq was done on old H3.3 and new H3.3 8h and 24h after 4OHT treatment.

2020-02-29 | GSE146120 | GEO

microRNA expression of K562, Me45 and HCT116, with or without p53 knock-out, treated with ionizing radiation.

Project description:MicroRNA expression was assessed in human cancer cells (K562, Me45, HCT116 wt and HCT116 p53-/-) treated with 4Gy of ionizing radiation. RNA was extracted from the cells 12 hours after irradiation and after 1h from non-treated controls.

2016-12-01 | E-MTAB-5197 | biostudies-arrayexpress

Aalysis of replication timing in the RPE1 and HCT116 cell line

Project description:Aalysis of replication timing in the RPE1 and HCT116 cell line

| PRJNA433397 | ENA

RPE1

Project description:Complex Rearrangements in RPE1

| PRJEB8037 | ENA

Transcription profiling of human cell lines K562, Me45 and HCT116 with or without p53 knock-out to study the effect of ionizing radiation

Project description:The objective of the experiment was to compare changes in the transcriptome induced by direct X-irradiation of cells, using the human K562 erythroleukemic, Me45 melanoma and HCT116 colon cancer cell lines. The transcript levels of K542 and Me45 RNA samples were measured using Affymetrix HG-U133A microarrays and for HCT116 with HGU 133A 2.0 in time points 1, 12, 24 h after irradiation and compared to the levels of RNA from control cells. Experiments were repeated twice for Me45 and K562 cells.

2010-09-28 | E-MEXP-2623 | biostudies-arrayexpress

Gene expression in hTERT-RPE1 cells with overexpression of MFRP

Project description:Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion.

2014-03-01 | GSE44540 | GEO

human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected

Project description:Transcriptional profiling of human hTERT-RPE1 cell spheroids comparing Control siRNA transfected hTERT-RPE1 cell spheroids with those transfected with YAP1 siRNA.

2015-02-01 | GSE54146 | GEO

RNA-seq in human RPE1-hTERT cells

Project description:Gene expression was profiled in unperturbed RPE1-hTERT cells by RNA-seq.

2020-02-29 | GSE146121 | GEO