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Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [CUT&RUN]

ABSTRACT: Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [CUT&RUN]

PROVIDER: PRJNA1160292 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30648971_1.fastq.gz	Fastqsanger.gz
	SRR30648971_2.fastq.gz	Fastqsanger.gz
	SRR30648972_1.fastq.gz	Fastqsanger.gz
	SRR30648972_2.fastq.gz	Fastqsanger.gz
	SRR31303234_1.fastq.gz	Fastqsanger.gz

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Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [CUT&RUN]

Project description:Alkylation repair homolog protein 5 (ALKBH5) binds to RNA and mediates RNA m6A demethylation. However, whether ALKBH5 binds to DNA is largely unknown. In this study, our CUT&RUN data show that ALKBH5 can bind to DNA in Hepa1-6 cells.

2025-02-27 | GSE277052 | GEO

Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [Cut&Tag]

Project description:Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [Cut&Tag]

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Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [Cut&Tag]

Project description:Alkylation repair homolog protein 5 (ALKBH5) binds to RNA and mediates RNA m6A demethylation. However, whether ALKBH5 binds to DNA is largely unknown. In this study, our CUT&Tag assay identified 12333 peaks in ALKBH5-overexpressing Hepa1-6 cells, indicating that ALKBH5 can bind to DNA.

2025-02-27 | GSE248568 | GEO

SIX2 genome-binding profiling by CUT&RUN and high-throughput sequencing

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CUT&RUN of PLAG1-S global chromatin occupancy in Lin-CD34+ cord-blood cells [Cut & Run]

Project description:To characterize genome-wide chromatin occupancy of the transcription factor PLAG1-S that could contribute to transient ex vivo expansion of human cord-blood (CB) derived HSC we performed CUT&RUN. 3 pools of Lin-CD34+ CB cells transduced to overexpress 3xFLAG-PLAG1-S were used for anti-FLAG or anti-IgG control enrichment of specific PLAG1-S DNA binding sites.

2022-12-01 | GSE181975 | GEO

CUT&RUN of PLAG1-S global chromatin occupancy in Lin-CD34+ cord-blood cells [Cut & Run]

Project description:CUT&RUN of PLAG1-S global chromatin occupancy in Lin-CD34+ cord-blood cells [Cut & Run]

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Cut&Run Analysis of ATF4 genomic occupancy in HCT116 colon carcinoma cell lines after treatment with DMSO, etoposide, tunicamycin, or histidinol

Project description:We examined ATF4 genomic occupancy resulting from treatment of HCT116 colon carcinoma human cell lines with DMSO, etoposide, histidinol, or, tunicamycin, using Cut&Run.

2022-08-20 | GSE211264 | GEO

CUT&RUN of erythroblasts upon perturbation of gamma-hemoglobin repressors (part II)

Project description:Through high throughput compound screening, we've identified compounds that induce the expression of fetal hemoglobin. This study contains CUT&RUN data of a novel target, WIZ.

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CUT&RUN Targeting SALL4 and HDAC2 in human melanoma cells

Project description:SALL4 builds a complex with histone deacetylases, and is thought to confer its effects epigenetically. Both loss of Sall4 and inhibition of HDAC2 leads to an invasiveness in human melanoma cells. To study co-occupancy of SALL4 and HDAC2, we employed CUT&RUN targeting SALL4 and HDAC2 in human melanoma cells (M010817).

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CUT&RUN of AML1-ETO binding occupancy following miR-130a KD in Kasumi-1 cells

Project description:We performed CUT&RUN assay with control Ig , anti-Flag and anti-ETO antibodies in Flag-tag AML1-ETO Kasumi-1 cells to determine binding occupancy of AML1-ETO following miR-130a KD. Kasumi-1 cells were transduced in 3 independent replicates for control and miR-130a KD samples with lentiviruses containing GFP reporter gene. GFP+ cells were sorted 3 days post-transduction and the cells were used in the CUT&RUN assay. Three antibodies were used for each biological sample.

| EGAS00001005867 | EGA

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