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Evaluation of the impact of genetically suppression of Nupr1 in aged vs young type 2 alveolar epithelial cells (AT2).

ABSTRACT: Evaluation of the impact of genetically suppression of Nupr1 in aged vs young type 2 alveolar epithelial cells (AT2).

PROVIDER: PRJNA1161177 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30668901.fastq.gz	Fastqsanger.gz
	SRR30668902.fastq.gz	Fastqsanger.gz
	SRR30668903.fastq.gz	Fastqsanger.gz
	SRR30668904.fastq.gz	Fastqsanger.gz
	SRR30668905.fastq.gz	Fastqsanger.gz

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Evaluation of the impact of genetically suppression of Nupr1 in aged vs young type 2 alveolar epithelial cells (AT2).

Project description:Our data demonstrated that Nupr1 has an age-specific function in the proliferation and differentiation of lung adenocarcinoma cells that are derived from AT2 cells. However, how Nupr1 impact AT2 cells in vivo has not been tested. Using somatic genetic approaches, we performed single-cell mRNA sequencing with primary AT2 cells with control or shRNAs targeting Nupr1.

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Evaluation of the impact of genetically suppression of DNA methyltransferase 1 (DNMT1) in aged vs young type 2 alveolar epithelial cells (AT2).

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Evaluation of the impact of genetically suppression of DNA methyltransferase 1 (DNMT1) in aged vs young type 2 alveolar epithelial cells (AT2).

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NUPR1 inhibits hyperPARylation-associated cell death

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Gene expression profile at single cell level of total alveolar type II (AT2) cells and CD44hi AT2 cells

Project description:AT2 cells are the resident progenitor cells in alveoli, capable of self-proliferation and differentiation into alveolar type I cells during homeostatic maintenance and tissue regeneration. The AT2 cell population is heterogenous. We identified a small subpopulation of AT2 cells that express high levels of CD44 (CD44hi) and display progenitor functions during alveoli homeostasis. To further analyze the heterogeneity of the AT2 cell population and characterize CD44hi AT2 cells, we performed single cell RNA-seq on the total AT2 cell population and CD44hi AT2 cells.

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