Rattus norvegicus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA116515 | ENA |
REPOSITORIES: ENA
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Project description:We examined the effect of grape seed extract (GSE), which are known to protect neurons against oxidative stress, on primary cultures of hippocampal astrocytes. GSE increased interleukin-6 (IL-6) expression. Microarrays are used to examine the effects of GSE on primary cultures of hippocampal neurons and astrocytes. Experiment Overall Design: Primary cultures of hippocampal neurons were treated with 0, 1, or 10 ug/ml of GSE for 24 hrs. Primary cultures of hippocampal astrocytes were treated with 0, 1, 10, or 100 ug/ml of GSE for 24 hrs.
2009-04-18 | E-GEOD-15285 | biostudies-arrayexpress
Project description:We examined the effect of grape seed extract (GSE), which are known to protect neurons against oxidative stress, on primary cultures of hippocampal astrocytes. GSE increased interleukin-6 (IL-6) expression. Microarrays are used to examine the effects of GSE on primary cultures of hippocampal neurons and astrocytes.
2009-04-09 | GSE15285 | GEO
Project description:A total of 37,185 transcripts were detected from RNA extracts from primary cultured astrocytes, and neurons isolated from Rattus hippocampus. After exposing astrocytes to insulin (200 nM) for 6h, 883 transcripts were upregulated 2SD or greater and 852 transcripts were downregulated 2SD or greater compared with vehicle treated control astrocyte cultures. This general pattern of gene expression was maintained for at least 12h in astrocytes where the expression of 811 genes were increased and 849 genes decreased compared with vehicle treated astrocytes. Informatic interrogation of genes with expression greater or less than 2 SD at the 6h and 12h time points in astrocytes identified signaling pathways associated with fatty acid activation, fatty acid β-oxidation, cAMP, gap junction, dendritic cell maturation ceramide degradation, triacylglycerol degradation and phospholipase activation. The functional association of gene changes in astrocytes after treatment with insulin for 6 and 12 h were almost exclusively related to the synthesis and storage of diacylglycerides, sterols, and other lipids. After exposing primary cultured Rattus hippocampal neurons to insulin (200 nM) for 6h, 753 genes were upregulated and 660 genes downregulated. This general pattern of gene expression was maintained for at least 12h when 768 genes were upregulated versus 736 genes downregulated in insulin treated neurons. Changes in gene expression profiles of neurons exposed to insulin for 6h, and 12h identified signaling pathways associated with actin-based motility, tight junctions, dendritic cell maturation, serotonin receptor, CREB (cAMP-response element binding protein), and axonal guidance. The functional association of gene changes in neurons after treatment with insulin for 6h, and 12h were largely related to neuroprotective/neurotrophic effects including tyrosine kinase tec-1 activation, synaptogenesis activation, and regulation of actin based cytoskeleton activation by the Rho family GTPases. These data suggest that insulin regulates neurotrophic signaling/functions in neurons and primary regulates lipid metabolism associated with fatty acid oxidation in astrocytes.
2025-03-13 | GSE280417 | GEO
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Project description:Inflammation is a key component of pathological angiogenesis. Here we induce cornea neovascularisation using sutures placed into the cornea, and sutures are removed to induce a regression phase. We used whole transcriptome microarray to monitor gene expression profies of several genes
2016-08-18 | GSE81418 | GEO
Project description:Human aortic endothelial cells were grown in culture until confluent. In three experiments using cells derived from three separate donors confluent cultures were incubated for 6 h with contol medium, or medium containing either extracts of oligomeric procyanidins from cranberry juice or red wine, or a procyanidin-rich grape seed extract. At the end of the 6 h treatment period conditioned media samples were retained for immunoassay of secreted peptides and proteins, and RNA was extracted for microarray analysis. Experiment Overall Design: Each experiment used cells from one donor. Treatment conditions were: control medium, cranberry extract (CRE), grape seed extract (GSE), and red wine extract (RWE).
2008-06-13 | E-GEOD-5556 | biostudies-arrayexpress
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