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RNA sequencing data of dural macrophages isolated from brains of Cx3cr1-GFP mice at different ages.

ABSTRACT: RNA sequencing data of dural macrophages isolated from brains of Cx3cr1-GFP mice at different ages.

PROVIDER: PRJNA1165151 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30792000.fastq.gz	Fastqsanger.gz
	SRR30792001.fastq.gz	Fastqsanger.gz
	SRR30792002.fastq.gz	Fastqsanger.gz
	SRR30792003.fastq.gz	Fastqsanger.gz
	SRR30792004.fastq.gz	Fastqsanger.gz

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RNA sequencing data of dural macrophages isolated from brains of Cx3cr1-GFP mice at different ages.

Project description:While macrophages in the meningeal compartments of the central nervous system (CNS) has been comprehensively characterized under steady state, studying their contribution to physiological and pathological processes has been severely hampered by the lack of specific targeting tools in vivo. Recent findings have shown that the dural sinus and its adjacent lymphatic vessels act as a neuroimmune interface. Notably, the cellular and functional heterogeneity of extrasinusoidal dural macrophages outside this immune hub is currently unclear. Therefore, we comprehensively characterized these cells using single-cell transcriptomics, fate mapping, confocal imaging, clonal analysis and transgenic mouse lines. Extrasinusoidal dural macrophages were clearly distinct from leptomeningeal and CNS parenchymal macrophages in terms of their origin, expansion kinetics and transcriptional profiles. Lastly, functional studies demonstrated that during autoimmune neuroinflammation, extrasinusoidal dural macrophages perform efferocytosis of granulocytes. Our results highlight a previously unappreciated myeloid cell diversity and provide insights into the brain’s innate immune system.

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Gene Expression in Cx3cr1-GFP+ Cells in PS2APP Alzheimer's Disease mice

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The dural innate immune barrier protects the CNS from systemic virus invasion [CD45_10X]

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Project description:GFP+CD45+CD11b+ cells were isolated from from heterozygous adult Cx3cr1-GFP/+ mouse hearts, spleens and brains by fluorescence activated cell sorting to prepare RNA for gene array analysis.<br>Three independently isolated populations GFP+ (CD45+CD11b+) cells for each tissue were used as replicates.

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Gene expression profile at single cell level of YS-derived cells and monocyte-derived cells from brain parenchyma and leptomeninges of Ms4a3-Cre: R26-TdTomato: Cx3cr1-gfp mice

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Project description:We hypothesize that under homeostatic as well as inflammatory conditions circulating monocytes and/or their bone marrow-derived progenitors might contribute to the replenishment of CD103+ and CD103- DC in lymphoid and non-lymphoid compartments. To that end, bone marrow cells from CX3CR1+/gfp C57BL/6 mice were sorted as follows: lineage negative (CD3, CD19, NK1.1, Ter119, Ly6G and CD11c) CX3CR1+c-kit+. Sorted cells were further cultured in vitro under the continuous presence of GM-CSF. lin-CX3CR1+c-kit+ bone marrow cells gave rise to CD103+ and CD103- DC in vitro. To test whether CD103 might be a suitable marker that allows the differentiation of functionally distinct DC subsets generated in vitro, CD11c+CD103+ and CD11c+CD103- DC were sorted from day 5 cultures of lin-CX3CR1+c-kit+ cells and analyzed by whole mouse genome microarrays from Agilent technologies. Compared to CD103+ DC, CD103- DC displayed a strong up-regulation of transcripts for genes involved in innate immunity, whereas those involved in costimulation were down-modulated. Our data suggest distinct functional activity of these two DC subsets. Keywords: Transcriptional profiling-Cell type comparison We compared the transcriptional profile of CD11c+CD103+ and CD11c+CD103- dendritic cells by use of Agilent Whole Mouse Genome Microarrays. Two sets of samples were generated independently as real biological replicates. The microarray analysis was performed as a dual-color experiment, including a dye-swap.

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